Concepts in Magnetic Resonance Part A (2011). Volume: 38A, Issue: 6. Pages: 280-288. Erik R.P. Zuiderweg, Aikaterini Rousaki et al.
TROSY, Transverse Relaxation Optimized Spectroscopy, was developed more than a decade ago. Since that time, the 15N-1H HSQC-TROSY experiment has become the standard “fingerprint” correlation spectrum for proteins of high molecular weight. In addition, its implementation in protein triple resonance experiments has pushed the boundaries of NMR assignment up to about 100 kDa, making NMR a highly relevant technique in structural biology. TROSY exploits the dipole-CSA cross-correlated relaxation properties of the NH system and selects for the narrowest of the HSQC J-correlation quartet in both dimensions. The original publications and reviews of TROSY use shift operators and/or single transition product operators to describe the TROSY coherence pathways selections. In this review, we offer a familiar Cartesian product operator approach to comprehensively describe all of the events in the modern TROSY pulse sequence such as multiplet selection, gradient coherence selection, gradient quadrature, and sensitivity enhancement.
[NMR paper] Gradient-enhanced TROSY described with Cartesian product operators
From Mendeley Biomolecular NMR group:
Gradient-enhanced TROSY described with Cartesian product operators
Concepts in Magnetic Resonance Part A (2011). Volume: 38A, Issue: 6. Pages: 280-288. Erik R.P. Zuiderweg, Aikaterini Rousaki et al.
TROSY, Transverse Relaxation Optimized Spectroscopy, was developed more than a decade ago. Since that time, the 15N-1H HSQC-TROSY experiment has become the standard “fingerprint” correlation spectrum for proteins of high molecular weight. In addition, its implementation in protein triple resonance experiments has pushed the boundaries of NMR...
nmrlearner
Journal club
0
08-24-2012 08:01 PM
Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
Abstract Here we describe phasing anomalies observed in gradient sensitivity enhanced 15N-1H HSQC spectra, and analyze their origin. It is shown that, as a result of 15N off-resonance effects, dispersive contributions to the 1H signal become detectable, and lead to 15N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented.
Content Type Journal Article
Category Article
Pages 199-207
nmrlearner
Journal club
0
09-30-2011 08:01 PM
Multiplet-filtered and gradient-selected zero-quantum TROSY experiments for 13C1H3 methyl groups in proteins
Multiplet-filtered and gradient-selected zero-quantum TROSY experiments for 13C1H3 methyl groups in proteins
Abstract Multiplet-filtered and gradient-selected heteronuclear zero-quantum coherence (gsHZQC) TROSY experiments are described for measuring 1Hâ??13C correlations for 13CH3 methyl groups in proteins. These experiments provide improved suppression of undesirable, broad outer components of the heteronuclear zero-quantum multiplet in medium-sized proteins, or in flexible sites of larger proteins, compared to previously described HZQC sequences (Tugarinov et al. in J Am Chem Soc...
[NMR paper] 3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR expe
3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR experiments with two sequential connectivity pathways and high sensitivity.
Related Articles 3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR experiments with two sequential connectivity pathways and high sensitivity.
J Biomol NMR. 2004 Mar;28(3):289-94
Authors: Ritter C, Lührs T, Kwiatkowski W, Riek R
The concept of chemical shift-coding monitors chemical shifts in multi-dimensional NMR experiments without additional polarization...
nmrlearner
Journal club
0
11-24-2010 09:25 PM
[NMR paper] Refocusing revisited: an optimized, gradient-enhanced refocused HSQC and its applicat
Refocusing revisited: an optimized, gradient-enhanced refocused HSQC and its applications in 2D and 3D NMR and in deuterium exchange experiments.
Related Articles Refocusing revisited: an optimized, gradient-enhanced refocused HSQC and its applications in 2D and 3D NMR and in deuterium exchange experiments.
J Biomol NMR. 1995 Jun;5(4):433-7
Authors: Davis JH
2D 15N-1H correlation spectra are ideal for measuring backbone amide populations to determine amide exchange protection factors in studies of protein folding or other structural features....
Gradient-enhanced TROSY described with Cartesian product operators
Linear Mode
