Due to the lack of genetically encoded probes for fluorine-19 nuclear magnetic resonance spectroscopy (^(19)F NMR), its utility for probing eukaryotic membrane protein dynamics is limited. Here we report an efficient method for the genetic incorporation of an unnatural amino acid (UAA), 3'-trifluoromenthyl-phenylalanine (mtfF), into cannabinoid receptor 1 (CB1) in the Baculovirus Expression System. The probe can be inserted at any environmentally sensitive site, while causing minimal structural...
[ASAP] A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1
A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1
Xiaoyan Wang?, Dongsheng Liu?, Ling Shen?, Fahui Li, Yongze Li, Lingyun Yang?, Tiandan Xu?, Houchao Tao?, Deqiang Yao?, Lijie Wu?, Kunio Hirata?, Laura M. Bohn, Alexandros Makriyannis×, Xiaohong Liu, Tian Hua?, Zhi-Jie Liu?, and Jiangyun Wang
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c06847/20211001/images/medium/ja1c06847_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.1c06847...
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[NMR paper] DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe.
DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe.
Related Articles DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe.
Nat Commun. 2020 Sep 25;11(1):4857
Authors: Liu Q, He QT, Lyu X, Yang F, Zhu ZL, Xiao P, Yang Z, Zhang F, Yang ZY, Wang XY, Sun P, Wang QW, Qu CX, Gong Z, Lin JY, Xu Z, Song SL, Huang SM, Guo SC, Han MJ, Zhu KK, Chen X, Kahsai AW, Xiao KH, Kong W, Li FH, Ruan...
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[NMR paper] A genetically encoded 19F NMR probe for lysine acetylation.
A genetically encoded 19F NMR probe for lysine acetylation.
A genetically encoded 19F NMR probe for lysine acetylation.
Chem Commun (Camb). 2018 Mar 29;:
Authors: Zhang F, Zhou Q, Yang G, An L, Li F, Wang J
Abstract
Advances in acetylated protein-protein/DNA interactions depend on the development of a novel NMR (nuclear magnetic resonance) probe to study the conformational changes of acetylated proteins. However, the method for detecting the acetylated protein conformation is underdeveloped. Herein, an acetyllysine mimic has...
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[NMR paper] Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Chem Sci. 2017 Nov 01;8(11):7631-7636
Authors: Roose BW, Zemerov SD, Dmochowski IJ
Abstract
Genetically encoded magnetic resonance imaging (MRI) contrast agents enable non-invasive detection of specific biomarkers in vivo. Here, we employed the hyper-CEST 129Xe NMR technique to quantify maltose (32 nM to 1 mM) through its modulation of conformational...
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[NMR paper] A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Related Articles A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Angew Chem Int Ed Engl. 2016 Jun 15;
Authors: Wang Y, Roose BW, Palovcak EJ, Carnevale V, Dmochowski IJ
Abstract
Molecular imaging holds considerable promise for elucidating biological processes in normal physiology as well as disease states, but requires noninvasive methods for identifying analytes at sub-micromolar...
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06-16-2016 12:06 PM
[NMR paper] Backbone NMR reveals allosteric signal transduction networks in the ?1-adrenergic receptor.
Backbone NMR reveals allosteric signal transduction networks in the ?1-adrenergic receptor.
Backbone NMR reveals allosteric signal transduction networks in the ?1-adrenergic receptor.
Nature. 2016 Feb 3;
Authors: Isogai S, Deupi X, Opitz C, Heydenreich FM, Tsai CJ, Brueckner F, Schertler GF, Veprintsev DB, Grzesiek S
Abstract
G protein-coupled receptors (GPCRs) are physiologically important transmembrane signalling proteins that trigger intracellular responses upon binding of extracellular ligands. Despite recent breakthroughs...
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[NMR paper] A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
Angew Chem Int Ed Engl. 2013 Feb 28;
Authors: Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J, Xiong Y, Sun J, Gong W, Tian C, Wang J
Abstract
Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein-protein interactions. The highly efficient genetic incorporation of...
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Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded
Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid.
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Angew Chem Int Ed Engl. 2010 Nov 25;
Authors: Nguyen TH, Ozawa K, Stanton-Cook M, Barrow R, Huber T, Otting G
A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1
Linear Mode
