[NMR paper] Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases
Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases
Substituting a single hydrogen atom in a protein by fluorine provides a probe for site-specific sensing by ^(19)F nuclear magnetic resonance (NMR) spectroscopy with minimal impact on the properties of the protein. Genetic encoding systems are presented for five different fluorinated analogues of phenylalanine: 2-, 3-, 4-fluorophenylalanine, 2,6-difluorophenylalanine, and 3,5-difluorophenylalanine. The systems allow the installation of each of these amino acids with high fidelity during in vivo...
[NMR paper] Genetic Encoding of Fluoro-l-tryptophans for Site-Specific Detection of Conformational Heterogeneity in Proteins by NMR Spectroscopy
Genetic Encoding of Fluoro-l-tryptophans for Site-Specific Detection of Conformational Heterogeneity in Proteins by NMR Spectroscopy
The substitution of a single hydrogen atom in a protein by fluorine yields a site-specific probe for sensitive detection by ^(19)F nuclear magnetic resonance (NMR) spectroscopy, where the absence of background signal from the protein facilitates the detection of minor conformational species. We developed genetic encoding systems for the site-selective incorporation of 4-fluorotryptophan, 5-fluorotryptophan, 6-fluorotryptophan, and 7-fluorotryptophan in...
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04-30-2024 10:50 PM
[NMR paper] Site-Specific Incorporation of 7-Fluoro-L-tryptophan into Proteins by Genetic Encoding to Monitor Ligand Binding by (19)F NMR Spectroscopy
Site-Specific Incorporation of 7-Fluoro-L-tryptophan into Proteins by Genetic Encoding to Monitor Ligand Binding by (19)F NMR Spectroscopy
A mutant aminoacyl-tRNA synthetase identified by a library selection system affords site-specific incorporation of 7-fluoro-L-tryptophan in response to an amber stop codon. The enzyme allows the production of proteins with a single hydrogen atom replaced by a fluorine atom as a sensitive nuclear magnetic resonance (NMR) probe. The substitution of a single hydrogen atom by another element that is as closely similar in size and hydrophobicity as possible...
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01-11-2022 06:11 PM
[NMR paper] Genetic Encoding of N6-(((Trimethylsilyl)methoxy)carbonyl)-l-lysine for NMR Studies of Protein-Protein and Protein-Ligand Interactions.
Genetic Encoding of N6-(((Trimethylsilyl)methoxy)carbonyl)-l-lysine for NMR Studies of Protein-Protein and Protein-Ligand Interactions.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Genetic Encoding of N6-(((Trimethylsilyl)methoxy)carbonyl)-l-lysine for NMR Studies of Protein-Protein and Protein-Ligand Interactions.
J Am Chem Soc. 2021 Jan 05;:
Authors: Abdelkader EH, Qianzhu H, Tan YJ, Adams LA, Huber T, Otting G
Abstract
Trimethylsilyl (TMS)...
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[ASAP] Genetic Encoding of N6-(((Trimethylsilyl)methoxy)carbonyl)-l-lysine for NMR Studies of Protein–Protein and Protein–Ligand Interactions
Genetic Encoding of N6-(((Trimethylsilyl)methoxy)carbonyl)-l-lysine for NMR Studies of Protein–Protein and Protein–Ligand Interactions
Elwy H. Abdelkader, Haocheng Qianzhu, Yi Jiun Tan, Luke A. Adams, Thomas Huber, and Gottfried Otting
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.0c11971/20210105/images/medium/ja0c11971_0013.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.0c11971
http://feeds.feedburner.com/~r/acs/jacsat/~4/IY4qiv61wDE
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[NMR paper] Conformational change study of dengue virus NS2B-NS3 protease using 19F NMR spectroscopy.
Conformational change study of dengue virus NS2B-NS3 protease using 19F NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Conformational change study of dengue virus NS2B-NS3 protease using 19F NMR spectroscopy.
Biochem Biophys Res Commun. 2015 Jun 12;461(4):677-80
Authors: Zhu L, Yang J, Li H, Sun H, Liu J, Wang J
Abstract
The dengue virus NS2B-NS3 protease (NS2B-NS3p), an important antiviral target for drug development,...
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08-02-2015 07:10 AM
[NMR paper] Conformational studies of neurohypophyseal hormones analogues with glycoconjugates by NMR spectroscopy.
Conformational studies of neurohypophyseal hormones analogues with glycoconjugates by NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Conformational studies of neurohypophyseal hormones analogues with glycoconjugates by NMR spectroscopy.
J Pept Sci. 2014 Jun;20(6):406-14
Authors: Lubecka EA, Sikorska E, Marcinkowska A, Ciarkowski J
Abstract
Two glycosylated peptides have been studied using NMR...
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12-30-2014 03:28 PM
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Chembiochem. 2010 Nov 22;11(17):2424-32
Authors: Díaz MD, Palomino-Schätzlein M, Corzana F, Andreu C, Carbajo RJ, del Olmo M, Canales-Mayordomo A, Pineda-Lucena A, Asensio G, Jiménez-Barbero J
The conformations of two synthetic pentapeptides with...
Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases
Hybrid Mode
