Related ArticlesGated electron transfers and electron pathways in azurin: a NMR dynamic study at multiple fields and temperatures.
J Mol Biol. 2004 Oct 1;342(5):1599-611
Authors: Zhuravleva AV, Korzhnev DM, Kupce E, Arseniev AS, Billeter M, Orekhov VY
Dynamic properties of electron transfer pathways in a small blue copper cupredoxin are explored using an extensive 15N NMR relaxation study of reduced Pseudomonas aeruginosa azurin at four magnetic fields (500-900 MHz) and at two temperatures chosen well below the melting point of the protein. Following a careful model-free analysis, several protein regions with different dynamic regimes are identified. Nanosecond time-scale mobility characterizes various residues of the hydrophobic surface patch believed to mark the natural entry point for electrons, notably the surface-exposed copper-ligand His117. These findings are consistent with a gated electron transfer process according to the "dynamic docking" model. Residues 47-49 along intramolecular pathways of electrons show rigidity that is remarkably conserved when increasing the temperature. Three different conformational exchange processes were observed in the millisecond range, one near the only disulfide bridge in the molecule and two near the copper ion. The latter two processes are consistent with previous data such as crystal structures at various pH values and NMR relaxation dispersion experiments; they may indicate an additional gated electron transfer mechanism at slower time-scales.
Effect of Freezing Conditions on Distances and Their Distributions Derived from Double Electron Electron Resonance (DEER): A Study of Doubly-Spin-Labeled T4 Lysozyme
Effect of Freezing Conditions on Distances and Their Distributions Derived from Double Electron Electron Resonance (DEER): A Study of Doubly-Spin-Labeled T4 Lysozyme
Publication year: 2012
Source: Journal of Magnetic Resonance, Available online 24 January 2012</br>
Elka R.*Georgieva, Aritro S.*Roy, Vladimir M.*Grigoryants, Petr P.*Borbat, Keith A.*Earle, ...</br>
Pulsed dipolar ESR spectroscopy, DEER and DQC, require frozen samples. An important issue in the biological application of this technique is how the freezing rate and concentration of cryoprotectant could possibly affect the...
nmrlearner
Journal club
0
01-25-2012 08:56 AM
[Stan NMR blog] Electron MR in Poland: a free collection of papers
Electron MR in Poland: a free collection of papers
An open-access set of 50+ EPR papers published in 'Current Topics in Biophysics'
Source: Stan blog library
nmrlearner
News from NMR blogs
0
12-09-2010 10:44 PM
[NMR paper] The dynamic properties of the M121H azurin metal site as studied by NMR of the parama
The dynamic properties of the M121H azurin metal site as studied by NMR of the paramagnetic Cu(II) and Co(II) metalloderivatives.
Related Articles The dynamic properties of the M121H azurin metal site as studied by NMR of the paramagnetic Cu(II) and Co(II) metalloderivatives.
J Biol Chem. 1998 Jan 2;273(1):177-85
Authors: Salgado J, Kroes SJ, Berg A, Moratal JM, Canters GW
The M121H azurin mutant in solution presents various species in equilibrium that can be detected and studied by 1H NMR of the Cu(II) and Co(II) paramagnetic...
nmrlearner
Journal club
0
11-17-2010 11:06 PM
[Stan NMR blog] Polish Electron Magnetic Resonance
Polish Electron Magnetic Resonance
Polish EPR Society becomes Polish EMR Group
Source: Stan blog library
nmrlearner
News from NMR blogs
0
10-22-2010 07:34 AM
[NMR paper] Characterisation of the electron self-exchange rates in hexametaphosphate-cytochrome-
Characterisation of the electron self-exchange rates in hexametaphosphate-cytochrome-c aggregates measured using high-resolution 1H-NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Characterisation of the electron self-exchange rates in hexametaphosphate-cytochrome-c aggregates measured using high-resolution 1H-NMR spectroscopy.
Eur J Biochem. 1991 Aug 1;199(3):553-60
Authors: Concar DW, Whitford D, Williams RJ
...
nmrlearner
Journal club
0
08-21-2010 11:12 PM
[NMR paper] Characterisation of the electron self-exchange rates in hexametaphosphate-cytochrome-
Characterisation of the electron self-exchange rates in hexametaphosphate-cytochrome-c aggregates measured using high-resolution 1H-NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Characterisation of the electron self-exchange rates in hexametaphosphate-cytochrome-c aggregates measured using high-resolution 1H-NMR spectroscopy.
Eur J Biochem. 1991 Aug 1;199(3):553-60
Authors: Concar DW, Whitford D, Williams RJ
...