The development of tools to follow and quantitate the fate of galactose in mammalian cells is crucial to the study and understanding of the inherited disorders of galactose metabolism. In this study we incubated normal human lymphoblasts with 1- or 2-(13)C galactose for 2.5 or 5 h and prepared TCA extracts of the cells. The various galactose metabolites were identified and quantified using a combination of proton, carbon and phosphorus NMR spectra. Galactose-1-phosphate (gal-1P), uridine diphosphogalactose, uridine diphosphoglucose and galactitol were present in the extracts. Average levels for gal-1P were around 10 nmol/mg protein and for uridine diphosphoglucose, uridine diphosphogalactose and galactitol in the range of 0.5-2 nmol/mg protein. Galactonate was never found in any conditions. Percentage labeling could be estimated for gal-1P and for the ribose carbons of AMP. The labeling agrees with a conversion of galactose to glucose through the Leloir pathway.
Skeletal muscle lipid metabolism studied by advanced magnetic resonance spectroscopy
Skeletal muscle lipid metabolism studied by advanced magnetic resonance spectroscopy
Publication year: 2012
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Available online 23 February 2012</br>
Arunima*Pola, Suresh Anand*Sadananthan, Jadegoud*Yaligar, Vijayasarathi*Nagarajan, Weiping*Han, ...</br>
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Role of aminotransferases in glutamate metabolism of human erythrocytes
Role of aminotransferases in glutamate metabolism of human erythrocytes
Abstract Human erythrocytes require a continual supply of glutamate to support glutathione synthesis, but are unable to transport this amino acid across their cell membrane. Consequently, erythrocytes rely on de novo glutamate biosynthesis from α-ketoglutarate and glutamine to maintain intracellular levels of glutamate. Erythrocytic glutamate biosynthesis is catalyzed by three enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glutamine aminohydrolase (GA). Although the presence of these...
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[NMR paper] Backbone dynamics of the human MIA protein studied by (15)N NMR relaxation: implicati
Backbone dynamics of the human MIA protein studied by (15)N NMR relaxation: implications for extended interactions of SH3 domains.
Related Articles Backbone dynamics of the human MIA protein studied by (15)N NMR relaxation: implications for extended interactions of SH3 domains.
Protein Sci. 2003 Mar;12(3):510-9
Authors: Stoll R, Renner C, Buettner R, Voelter W, Bosserhoff AK, Holak TA
The melanoma inhibitory activity (MIA) protein is a clinically valuable marker in patients with malignant melanoma as enhanced values diagnose metastatic...
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[NMR paper] Glucose and glutamine metabolism in C6 glioma cells studied by carbon 13 NMR.
Glucose and glutamine metabolism in C6 glioma cells studied by carbon 13 NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Glucose and glutamine metabolism in C6 glioma cells studied by carbon 13 NMR.
Biochimie. 1996;78(3):155-64
Authors: Portais JC, Voisin P, Merle M, Canioni P
The question as to whether glutamine and glucose are both required for optimal growth of glioma cells is studied through the role of these substrates on the metabolism of the cells. C6 rat...
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[NMR paper] 31P and 1H NMR studies of ethanolamine-linked phosphoglycerides metabolism in human T
31P and 1H NMR studies of ethanolamine-linked phosphoglycerides metabolism in human T lymphocytes.
Related Articles 31P and 1H NMR studies of ethanolamine-linked phosphoglycerides metabolism in human T lymphocytes.
Cell Mol Biol. 1991;37(7):705-11
Authors: Ricciolini R, Miccheli A, Peluso G, Delfini M, Conti F
Aqueous and organic extracts of peripheral human T lymphocytes and of T lymphoblastoid cell lines have been examinated by 31P and 1H NMR spectroscopy in order to study the metabolism of ethanolamine (Etn) linked phosphoglycerides. The...
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[NMR paper] Structure-function relationships in human epidermal growth factor studied by site-dir
Structure-function relationships in human epidermal growth factor studied by site-directed mutagenesis and 1H NMR.
Related Articles Structure-function relationships in human epidermal growth factor studied by site-directed mutagenesis and 1H NMR.
Biochemistry. 1991 Sep 10;30(36):8891-8
Authors: Hommel U, Dudgeon TJ, Fallon A, Edwards RM, Campbell ID
In order to elucidate the mechanism of interaction between human epidermal growth factor (EGF) and its receptor, selected variants of EGF, differing by single amino acid substitutions, have been...
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[NMR paper] Structure-function relationships in human epidermal growth factor studied by site-dir
Structure-function relationships in human epidermal growth factor studied by site-directed mutagenesis and 1H NMR.
Related Articles Structure-function relationships in human epidermal growth factor studied by site-directed mutagenesis and 1H NMR.
Biochemistry. 1991 Sep 10;30(36):8891-8
Authors: Hommel U, Dudgeon TJ, Fallon A, Edwards RM, Campbell ID
In order to elucidate the mechanism of interaction between human epidermal growth factor (EGF) and its receptor, selected variants of EGF, differing by single amino acid substitutions, have been...
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[NMR paper] Hypophosphite transport in human erythrocytes studied by overdetermined one-dimension
Hypophosphite transport in human erythrocytes studied by overdetermined one-dimensional NMR exchange analysis.
Related Articles Hypophosphite transport in human erythrocytes studied by overdetermined one-dimensional NMR exchange analysis.
NMR Biomed. 1990 Apr;3(2):59-63
Authors: Price WS, Kuchel PW
The membrane transport kinetics of the disubstituted phosphorus oxyacid, hypophosphite, were studied in human red cells under equilibrium exchange conditions. Hypophosphite is an analogue of both the bicarbonate and phosphate ions and is known to be...
Galactose metabolism in normal human lymphoblasts studied by (1)H, (13)C and (31)P NM
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