Related ArticlesGadolinium Complexes as Contrast Agent for Cellular NMR Spectroscopy.
Int J Mol Sci. 2020 Jun 05;21(11):
Authors: Sakol N, Egawa A, Fujiwara T
Abstract
Aqua Gd3+ and Gd-DOTA (gadolinium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacete) complexes were studied as a contrast agent in cellular NMR (nuclear magnetic resonance) spectroscopy for distinguishing between intracellular and extracellular spaces. The contrast agents for this purpose should provide strong paramagnetic relaxation enhancement and localize in the extracellular space without disturbing biological functions. Cell membrane permeability to Gd complexes was evaluated from the concentrations of gadolinium complexes in the inside and outside of E. coli cells measured by the 1H-NMR relaxation. The site-specific binding of the complexes to E. coli cells was also analyzed by high-resolution solid-state 13C-NMR. The aqua Gd3+ complex did not enhance T1 relaxation in proportion to the amount of added Gd3+. This Gd3+ concentration dependence and the 13C-NMR indicated that its strong cytotoxicity should be due to the binding of the paramagnetic ions to cellular components especially at the lipid membranes. In contrast, Gd-DOTA stayed in the solution states and enhanced relaxation in proportion to the added amount. This agent exhibited strong T1 contrast between the intra- and extracellular spaces by a factor of ten at high concentrations under which the cells were viable over a long experimental time of days. These properties make Gd-DOTA suitable for selectively contrasting the living cellular space in NMR spectroscopy primarily owing to its weak interaction with cellular components.
Gadolinium based endohedral metallofullerene Gd2@C79N as a relaxation boosting agent for dissolution DNP at high fields
From The DNP-NMR Blog:
Gadolinium based endohedral metallofullerene Gd2@C79N as a relaxation boosting agent for dissolution DNP at high fields
Wang, X., et al., Gadolinium based endohedral metallofullerene Gd2@C79N as a relaxation boosting agent for dissolution DNP at high fields. Chem Commun (Camb), 2018. 54(19): p. 2425-2428.
https://www.ncbi.nlm.nih.gov/pubmed/29457159
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05-08-2018 02:39 AM
[NMR paper] Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Chem Sci. 2017 Nov 01;8(11):7631-7636
Authors: Roose BW, Zemerov SD, Dmochowski IJ
Abstract
Genetically encoded magnetic resonance imaging (MRI) contrast agents enable non-invasive detection of specific biomarkers in vivo. Here, we employed the hyper-CEST 129Xe NMR technique to quantify maltose (32 nM to 1 mM) through its modulation of conformational...
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03-24-2018 12:21 PM
Bis-Gadolinium Complexes for Solid Effect and Cross Effect Dynamic Nuclear Polarization #DNPNMR
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Bis-Gadolinium Complexes for Solid Effect and Cross Effect Dynamic Nuclear Polarization #DNPNMR
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Kaushik, M., et al., Bis-Gadolinium Complexes for Solid Effect and Cross Effect Dynamic Nuclear Polarization. Angew Chem Int Ed Engl, 2017. 56(15): p. 4295-4299.
https://www.ncbi.nlm.nih.gov/pubmed/28319293
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07-15-2017 05:05 PM
Assessment of a Heuristic Model for Characterization of Magnetic Nanoparticles as Contrast Agent in MRI
From The DNP-NMR Blog:
Assessment of a Heuristic Model for Characterization of Magnetic Nanoparticles as Contrast Agent in MRI
Félix-González, N., et al., Assessment of a Heuristic Model for Characterization of Magnetic Nanoparticles as Contrast Agent in MRI. Concepts in Magnetic Resonance Part A, 2015. 44A(5): p. 279-286.
http://dx.doi.org/10.1002/cmr.a.21361
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08-03-2016 05:31 PM
Long-lived spin states as a source of contrast in magnetic resonance spectroscopy and imaging
From The DNP-NMR Blog:
Long-lived spin states as a source of contrast in magnetic resonance spectroscopy and imaging
Kiryutin, A.S., et al., Long-lived spin states as a source of contrast in magnetic resonance spectroscopy and imaging. J Magn Reson, 2015. 261: p. 64-72.
http://www.ncbi.nlm.nih.gov/pubmed/26529204
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03-25-2016 04:12 PM
Cucurbit[6]uril is an ultrasensitive (129)Xe NMR contrast agent
From The DNP-NMR Blog:
Cucurbituril is an ultrasensitive (129)Xe NMR contrast agent
Wang, Y. and I.J. Dmochowski, Cucurbituril is an ultrasensitive (129)Xe NMR contrast agent. Chem Commun (Camb), 2015. 51(43): p. 8982-5.
http://www.ncbi.nlm.nih.gov/pubmed/25929681
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06-11-2015 05:15 AM
[NMR paper] Gadolinium leakage into subarachnoid space and cystic metastases.
Gadolinium leakage into subarachnoid space and cystic metastases.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.dirjournal.org-images-pubmed.jpg http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.dirjournal.org-images-pubmed.jpg Related Articles Gadolinium leakage into subarachnoid space and cystic metastases.
Diagn Interv Radiol. 2013 Jan 21;
Authors: Yildiz AE, Atli E, Oguz KK
Abstract
Subarachnoid space (SAS) and cystic metastatic lesions of brain parenchyma appear hypointense on fluid-attenuated...
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02-03-2013 10:19 AM
Magneto-optical contrast in liquid-state optically detected NMR spectroscopy [Applied Physical Sciences]
Magneto-optical contrast in liquid-state optically detected NMR spectroscopy
Pagliero, D., Meriles, C. A....
Date: 2011-12-06
We use optical Faraday rotation (OFR) to probe nuclear spins in real time at high-magnetic field in a range of diamagnetic sample fluids. Comparison of OFR-detected NMR spectra reveals a correlation between the relative signal amplitude and the fluid Verdet constant, which we interpret as a manifestation of the variable detuning between the probe beam and the sample optical transitions. The analysis of chemical-shift-resolved, optically detected spectra allows...
Gadolinium Complexes as Contrast Agent for Cellular NMR Spectroscopy.
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