[NMR paper] G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling
G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling
G protein-coupled receptors (GPCRs) are a large membrane protein family found in higher organisms, including the human body. GPCRs mediate cellular responses to diverse extracellular stimuli and thus control key physiological functions, which makes them important targets for drug design. Signaling by GPCRs is related to the structure and dynamics of these proteins, which are modulated by extrinsic ligands as well as by intracellular binding partners such as G proteins and arrestins. Here, we...
Efficient production of a functional G protein-coupled receptor in E. coli for structural studies
Efficient production of a functional G protein-coupled receptor in E. coli for structural studies
Abstract
G protein-coupled receptors (GPCRs) are transmembrane signal transducers which regulate many key physiological process. Since their discovery, their analysis has been limited by difficulties in obtaining sufficient amounts of the receptors in high-quality, functional form from heterologous expression hosts. Albeit highly attractive because of its simplicity and the ease of isotope labeling for NMR studies, heterologous expression of functional...
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01-27-2021 04:52 AM
[ASAP] Dynamic G Protein-Coupled Receptor Signaling Probed by Solution NMR Spectroscopy
Dynamic G Protein-Coupled Receptor Signaling Probed by Solution NMR Spectroscopy
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00032/20200302/images/medium/bi0c00032_0014.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00032
http://feeds.feedburner.com/~r/acs/bichaw/~4/4bPBV3Rv0UE
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03-03-2020 11:18 PM
[NMR paper] Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies.
Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies.
Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies.
Biochim Biophys Acta. 2018 Mar 26;:
Authors: Bumbak F, Keen AC, Gunn NJ, Gooley PR, Bathgate RAD, Scott DJ
Abstract
Neurotensin is a 13-residue peptide that acts as a neuromodulator of classical neurotransmitters such as dopamine and glutamate in the mammalian central nervous system, mainly by...
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03-30-2018 06:40 PM
Structural Basis for G Protein-Coupled Receptor Activation
Structural Basis for G Protein-Coupled Receptor Activation
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00747/20171010/images/medium/bi-2017-00747c_0005.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00747
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/AO4DRBB8hkc
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10-10-2017 09:37 PM
[NMR paper] Solution NMR studies reveal the location of the second transmembrane domain of the human sigma-1 receptor.
Solution NMR studies reveal the location of the second transmembrane domain of the human sigma-1 receptor.
Related Articles Solution NMR studies reveal the location of the second transmembrane domain of the human sigma-1 receptor.
FEBS Lett. 2015 Jan 31;
Authors: Ortega-Roldan JL, Ossa F, Amin NT, Schnell JR
Abstract
The sigma-1 receptor (S1R) is a ligand-regulated membrane chaperone protein associated with endoplasmic reticulum stress response, and modulation of ion channel activities at the plasma membrane. We report here...
[NMR paper] Expression, purification and reconstitution of the C-terminal transmembrane domain of scavenger receptor BI into detergent micelles for NMR analysis.
Expression, purification and reconstitution of the C-terminal transmembrane domain of scavenger receptor BI into detergent micelles for NMR analysis.
Expression, purification and reconstitution of the C-terminal transmembrane domain of scavenger receptor BI into detergent micelles for NMR analysis.
Protein Expr Purif. 2014 Nov 12;
Authors: Chadwick AC, Jensen DR, Peterson FC, Volkman BF, Sahoo D
Abstract
Scavenger receptor class B type I (SR-BI), the high density lipoprotein (HDL) receptor, is important for the delivery of...
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12-03-2014 04:05 PM
Expression, purification, and reconstitution of the transmembrane domain of the human amyloid precursor protein for NMR studies.
Expression, purification, and reconstitution of the transmembrane domain of the human amyloid precursor protein for NMR studies.
Expression, purification, and reconstitution of the transmembrane domain of the human amyloid precursor protein for NMR studies.
Protein Expr Purif. 2011 Aug 31;
Authors: Chen W, Gamache E, Richardson D, Du Z, Wang C
Abstract
Alzheimer's disease (AD) is the most common type of dementia in elderly people. Senile plaques, a pathologic hallmark of AD, are composed of amyloid ? peptide (A?). A? aggregation produces...
G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling
Linear Mode
