Related ArticlesFull 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharides from Neisseria meningitidis used in vaccine production.
Carbohydr Res. 1996 Dec 24;296:83-96
Authors: Lemercinier X, Jones C
We report essentially complete 1H NMR assignments for the capsular polysaccharides from Neisseria meningitidis serotypes A, C, W-135, and Y. These polysaccharides are components of current polysaccharide vaccines against meningococcal infection and of the polysaccharide-protein conjugate vaccines under development. From these NMR data the pattern of O-acetylation was determined. O-Acetylation of the W-135 polysaccharide is reported for the first time. We also show that, for the Types C and W-135 polysaccharides a migration of O-acetyl groups occurs during storage in solution, and demonstrate that high field 1H NMR represents a simple and sensitive method to define the O-acetylation pattern of individual batches of these polysaccharides.
[NMR paper] Correlation of the sweetness of variants of the protein brazzein with patterns of hyd
Correlation of the sweetness of variants of the protein brazzein with patterns of hydrogen bonds detected by NMR spectroscopy.
Related Articles Correlation of the sweetness of variants of the protein brazzein with patterns of hydrogen bonds detected by NMR spectroscopy.
J Biol Chem. 2003 Aug 15;278(33):31331-9
Authors: Assadi-Porter FM, Abildgaard F, Blad H, Markley JL
In sequence-function investigations, approaches are needed for rapidly screening protein variants for possible changes in conformation. Recent NMR methods permit direct...
nmrlearner
Journal club
0
11-24-2010 09:01 PM
[NMR paper] NMR assignment of the full-length ribosomal protein L11 from Thermotoga maritima.
NMR assignment of the full-length ribosomal protein L11 from Thermotoga maritima.
Related Articles NMR assignment of the full-length ribosomal protein L11 from Thermotoga maritima.
J Biomol NMR. 2003 Feb;25(2):163-4
Authors: Ilin S, Hoskins A, Schwalbe H, Wöhnert J
nmrlearner
Journal club
0
11-24-2010 09:01 PM
[NMR paper] An NMR spectroscopic identity test for the control of the capsular polysaccharide fro
An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b.
Related Articles An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b.
Biologicals. 2000 Sep;28(3):175-83
Authors: Lemercinier X, Jones C
We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to control the identity of purified bulk capsular polysaccharide from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib...
nmrlearner
Journal club
0
11-19-2010 08:29 PM
[NMR paper] Full 1H NMR assignment of a 24-nucleotide RNA hairpin: application of the 1H 3D-NOE/2
Full 1H NMR assignment of a 24-nucleotide RNA hairpin: application of the 1H 3D-NOE/2QC experiment.
Related Articles Full 1H NMR assignment of a 24-nucleotide RNA hairpin: application of the 1H 3D-NOE/2QC experiment.
J Biomol NMR. 1998 Apr;11(3):319-28
Authors: Nooren IM, Wang KY, Borer PN, Pelczer I
The subject RNA models the binding site for the coat protein of the R17 virus, as well as the ribosome recognition sequence for the R17 replicase gene. With an RNA of this size, overlaps among the sugar protons complicate assignments of the 1H NMR...
nmrlearner
Journal club
0
11-17-2010 11:06 PM
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution NMR Spectroscopy.
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution NMR Spectroscopy.
J Am Chem Soc. 2010 Oct 1;
Authors: Liokatis S, Dose A, Schwarzer D, Selenko P
Post-translational protein modifications (PTMs) such as phosphorylation and acetylation regulate a large number of eukaryotic signaling processes. In most instances, it is the combination of different PTMs that "encode" the biological outcome of these covalent...
nmrlearner
Journal club
0
10-05-2010 12:11 PM
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution NMR Spectroscopy
Stamatios Liokatis, Alexander Dose, Dirk Schwarzer and Philipp Selenko
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja106764y/aop/images/medium/ja-2010-06764y_0002.gif
Journal of the American Chemical Society
DOI: 10.1021/ja106764y
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/jfhp2Sg-hpY
nmrlearner
Journal club
0
10-02-2010 12:16 AM
[NMR paper] Full assignment of heme redox potentials of cytochrome c3 of D. vulgaris Miyazaki F b
Full assignment of heme redox potentials of cytochrome c3 of D. vulgaris Miyazaki F by 1H-NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Full assignment of heme redox potentials of cytochrome c3 of D. vulgaris Miyazaki F by 1H-NMR.
FEBS Lett. 1991 Jul 8;285(1):149-51
Authors: Park JS, Kano K, Niki K, Akutsu H
Site-specific heme assignment of the 1H-NMR spectrum of cytochrome c3 of D. vulgaris Miyazaki F, a tetraheme protein, was established. The major reduction...
nmrlearner
Journal club
0
08-21-2010 11:12 PM
[NMR paper] Full assignment of heme redox potentials of cytochrome c3 of D. vulgaris Miyazaki F b
Full assignment of heme redox potentials of cytochrome c3 of D. vulgaris Miyazaki F by 1H-NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Full assignment of heme redox potentials of cytochrome c3 of D. vulgaris Miyazaki F by 1H-NMR.
FEBS Lett. 1991 Jul 8;285(1):149-51
Authors: Park JS, Kano K, Niki K, Akutsu H
Site-specific heme assignment of the 1H-NMR spectrum of cytochrome c3 of D. vulgaris Miyazaki F, a tetraheme protein, was established. The major reduction...
Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharide
Linear Mode
