The focus of this project is to take advantage of the large NMR chemical shift anisotropy of 19F to determine the orientation of fluorine labeled biomolecules in situ in oriented biological systems such as muscle. The difficulty with a single fluorine atom is that the orientation determined from a chemical shift is not singlevalued in the case of a fully anisotropic chemical shift tensor. The utility of a labeling approach with two fluorine labels in a fixed molecular framework where one of the labels has an axially symmetric chemical shift anisotropy such as a CF3 group and the other has a fully asymmetric chemical shift anisotropy such as 5-fluorotryptophan is evaluated. The result is that the orientation of the label can be determined straightforwardly from a single one-dimensional 19F NMR spectrum. The potential applications are widespread and not limited to biological applications.
[NMR paper] Specific isotopic labelling and reverse labelling for protein NMR spectroscopy: using metabolic precursors in sample preparation
Specific isotopic labelling and reverse labelling for protein NMR spectroscopy: using metabolic precursors in sample preparation
The study of protein structure, dynamics and function by NMR spectroscopy commonly requires samples that have been enriched ('labelled') with the stable isotopes 13C and/or 15N. The standard approach is to uniformly label a protein with one or both of these nuclei such that all C and/or N sites are in principle 'NMR-visible'. NMR spectra of uniformly labelled proteins can be highly complicated and suffer from signal overlap. Moreover, as molecular size increases...
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11-17-2022 11:19 AM
[NMR paper] The precious fluorine on the ring: fluorine NMR for biological systems.
The precious fluorine on the ring: fluorine NMR for biological systems.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles The precious fluorine on the ring: fluorine NMR for biological systems.
J Biomol NMR. 2020 Jul 10;:
Authors: Boeszoermenyi A, Ogórek B, Jain A, Arthanari H, Wagner G
Abstract
The fluorine-19 nucleus was recognized early to harbor exceptional properties for NMR spectroscopy. With 100% natural abundance, a high...
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07-13-2020 01:56 AM
The precious fluorine on the ring: fluorine NMR for biological systems
The precious fluorine on the ring: fluorine NMR for biological systems
Abstract
The fluorine-19 nucleus was recognized early to harbor exceptional properties for NMR spectroscopy. With 100% natural abundance, a high gyromagnetic ratio (83% sensitivity compared to 1H), a chemical shift that is extremely sensitive to its surroundings and near total absence in biological systems, it was destined to become a favored NMR probe, decorating small and large molecules. However, after early excitement, where uptake of fluorinated aromatic amino acids was...
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07-10-2020 08:40 PM
Optimizing fluorine labelling for 19 F solid-state NMR in oriented biological systems
Optimizing fluorine labelling for 19 F solid-state NMR in oriented biological systems
Abstract
When planning a fluorine labeling strategy for 19F solid state NMR (ssNMR) studies of the structure and/or mobility of fluorine labeled compounds in situ in an oriented biological system, it is important to characterize the NMR properties of the label. This manuscript focuses on the characterization of a selection of aromatic fluorine compounds in dimyristoylphosphatidylcholine bilayers using 19F ssNMR from the standpoint of determiningÂ*the optimum...
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02-29-2020 09:52 PM
[ASAP] Unraveling the Reaction Mechanisms of SiO Anodes for Li-Ion Batteries by Combining in Situ 7Li and ex Situ 7Li/29Si Solid-State NMR Spectroscopy
Unraveling the Reaction Mechanisms of SiO Anodes for Li-Ion Batteries by Combining in Situ 7Li and ex Situ 7Li/29Si Solid-State NMR Spectroscopy
Keitaro Kitada, Oliver Pecher, Pieter C. M. M. Magusin, Matthias F. Groh, Robert S. Weatherup, Clare P. Grey
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.9b01589/20190423/images/medium/ja-2019-01589u_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.9b01589
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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04-23-2019 07:54 PM
[NMR paper] Weak Intermolecular Hydrogen Bonds with Fluorine: Detection and Implications for Enzymatic/Chemical Reactions, Chemical Properties, and Ligand/Protein Fluorine NMR Screening.
Weak Intermolecular Hydrogen Bonds with Fluorine: Detection and Implications for Enzymatic/Chemical Reactions, Chemical Properties, and Ligand/Protein Fluorine NMR Screening.
Related Articles Weak Intermolecular Hydrogen Bonds with Fluorine: Detection and Implications for Enzymatic/Chemical Reactions, Chemical Properties, and Ligand/Protein Fluorine NMR Screening.
Chemistry. 2016 Apr 26;
Authors: Dalvit C, Vulpetti A
Abstract
It is known that strong hydrogen-bonding interactions play an important role in many chemical and...
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04-27-2016 01:51 PM
In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization
From The DNP-NMR Blog:
In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization
Barskiy, D.A., et al., In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization. ChemPhysChem, 2014. 15(18): p. 4100-7.
http://www.ncbi.nlm.nih.gov/pubmed/25367202
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12-15-2014 03:31 PM
Erratum to: Paramagnetic labelling of proteins and oligonucleotides for NMR
Erratum to: Paramagnetic labelling of proteins and oligonucleotides for NMR
Erratum to: Paramagnetic labelling of proteins and oligonucleotides for NMR
Content Type Journal Article
Pages 1-2
DOI 10.1007/s10858-011-9475-7
Authors
Fluorine labelling for in situ 19F NMR in oriented systems
Linear Mode
