The incorporation of fluorine atoms into a biomacromolecule provides a background-free and environmentally sensitive reporter of structure, conformation and interactions using 19F NMR. There are several methods to introduce the 19F reporter - either by synthetic incorporation via solid phase peptide synthesis; by suppressing the incorporation or biosynthesis of a natural amino acid and supplementing the growth media with a fluorinated counterpart during protein expression; and by genetic code...
[NMR paper] Fluorinated Aromatic Amino Acids are Sensitive 19F NMR Probes for Bromodomain-Ligand Interactions.
Fluorinated Aromatic Amino Acids are Sensitive 19F NMR Probes for Bromodomain-Ligand Interactions.
Related Articles Fluorinated Aromatic Amino Acids are Sensitive 19F NMR Probes for Bromodomain-Ligand Interactions.
ACS Chem Biol. 2014 Oct 7;
Authors: Mishra NK, Urick AK, Ember S, Schonbrunn E, Pomerantz WC
Abstract
We describe a 19F NMR method for detecting bromodomain-ligand interactions using fluorine-labeled aromatic amino acids due to the conservation of aromatic residues in the bromodomain binding site. We test the...
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[NMR paper] 19F NMR study on the complex of fluorinated vitamin D derivatives with vitamin D receptor: elucidation of the conformation of vitamin D ligands accommodated in the receptor.
19F NMR study on the complex of fluorinated vitamin D derivatives with vitamin D receptor: elucidation of the conformation of vitamin D ligands accommodated in the receptor.
Related Articles 19F NMR study on the complex of fluorinated vitamin D derivatives with vitamin D receptor: elucidation of the conformation of vitamin D ligands accommodated in the receptor.
J Med Dent Sci. 2011;58(4):103-12
Authors: Morizono D
Abstract
Nuclear receptors mediate allosteric communications where ligand binding initiates a cascade of signal...
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A comparative study of 1H and 19F Overhauser DNP in fluorinated benzenes
From The DNP-NMR Blog:
A comparative study of 1H and 19F Overhauser DNP in fluorinated benzenes
Neudert, O., et al., A comparative study of 1H and 19F Overhauser DNP in fluorinated benzenes. Phys Chem Chem Phys, 2013. 15(47): p. 20717-26.
http://www.ncbi.nlm.nih.gov/pubmed/24192645
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01-14-2014 02:57 AM
A comparative study of (1)H and (19)F Overhauser DNP in fluorinated benzenes
From The DNP-NMR Blog:
A comparative study of (1)H and (19)F Overhauser DNP in fluorinated benzenes
Neudert, O., et al., A comparative study of (1)H and (19)F Overhauser DNP in fluorinated benzenes. Phys Chem Chem Phys, 2013. 15(47): p. 20717-26.
http://www.ncbi.nlm.nih.gov/pubmed/24192645
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11-28-2013 05:07 AM
[NMR paper] Fluorinated Carbohydrates as Lectin Ligands: Dissecting Glycan-Cyanovirin Interactions by Using 19 F NMR Spectroscopy.
Fluorinated Carbohydrates as Lectin Ligands: Dissecting Glycan-Cyanovirin Interactions by Using 19 F NMR Spectroscopy.
Fluorinated Carbohydrates as Lectin Ligands: Dissecting Glycan-Cyanovirin Interactions by Using 19 F NMR Spectroscopy.
Chemistry. 2013 Feb 28;
Authors: Matei E, André S, Glinschert A, Infantino AS, Oscarson S, Gabius HJ, Gronenborn AM
Abstract
NMR spectroscopy and isothermal titration calorimetry (ITC) are powerful methods to investigate ligand-protein interactions. Here, we present a versatile and sensitive fluorine NMR...
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[NMR paper] NMR probing of protein-protein interactions using reporter ligands and affinity tags.
NMR probing of protein-protein interactions using reporter ligands and affinity tags.
Related Articles NMR probing of protein-protein interactions using reporter ligands and affinity tags.
J Am Chem Soc. 2004 Feb 18;126(6):1636-7
Authors: Ludwiczek ML, Baminger B, Konrat R
A novel method is proposed for the detection and quantification of protein-protein interactions in solution. In this approach, one protein binding partner is tagged with a ligand binding domain, and protein-protein interaction is monitored via changes in the NMR relaxation...
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[NMR paper] The use of 19F NMR in the study of protein alkylation by fluorinated reactive interme
The use of 19F NMR in the study of protein alkylation by fluorinated reactive intermediates.
Related Articles The use of 19F NMR in the study of protein alkylation by fluorinated reactive intermediates.
Adv Exp Med Biol. 1991;283:735-8
Authors: Harris JW, Anders MW
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[NMR paper] NMR studies of retinoid-protein interactions: the conformation of [13C]-beta-ionones
NMR studies of retinoid-protein interactions: the conformation of -beta-ionones bound to beta-lactoglobulin B.
Related Articles NMR studies of retinoid-protein interactions: the conformation of -beta-ionones bound to beta-lactoglobulin B.
Pharm Res. 1999 May;16(5):651-9
Authors: Curley RW, Sundaram AK, Fowble JW, Abildgaard F, Westler WM, Markley JL
PURPOSE: Vitamin A (retinol) and its metabolites comprise the natural retinoids. While the biological action of these molecules are thought to be primarily mediated by ca. 55 kDa nuclear retinoic...
Fluorinated tags to study protein conformation and interactions using 19F NMR
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