Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
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Bioscience Technology
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Visualizing RNA-protein Interactions in Live Human Cells
Bioscience Technology
UAlbany chemists Alan Chen Ph.D, Maksim Royzen Ph.D, and Alex Shekhtman Ph.D have combined their labs on a collaborative project aimed at visualizing RNA-protein interactions inside live human cells by using multi-dimensional NMR (nuclear ...
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06-03-2017 11:49 AM
[NMR paper] A general mechanism of photoconversion of green-to-red fluorescent proteins based on blue and infrared light reduces phototoxicity in live-cell single-molecule imaging
A general mechanism of photoconversion of green-to-red fluorescent proteins based on blue and infrared light reduces phototoxicity in live-cell single-molecule imaging
Photoconversion of fluorescent proteins by blue and complementary near-infrared light, termed primed conversion (PC), is a mechanism recently discovered for Dendra2. We demonstrate that controlling the conformation of arginine at residue 66 by threonine at residue 69 of fluorescent proteins from Anthozoan families (Dendra2, mMaple, Eos, mKikGR, pcDronpa protein families) represents a general route to facilitate PC....
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06-02-2017 08:33 PM
The Cation-? Interaction Enables a Halo-TagFluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-FreeProtein Quantification
The Cation-? Interaction Enables a Halo-TagFluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-FreeProtein Quantification
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00056/20170308/images/medium/bi-2017-00056j_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00056
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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[NMR paper] Beyond a fluorescent probe: Inhibition of cell division protein FtsZ by mant-GTP elucidated by NMR and biochemical approaches.
Beyond a fluorescent probe: Inhibition of cell division protein FtsZ by mant-GTP elucidated by NMR and biochemical approaches.
Related Articles Beyond a fluorescent probe: Inhibition of cell division protein FtsZ by mant-GTP elucidated by NMR and biochemical approaches.
ACS Chem Biol. 2015 Aug 6;
Authors: Huecas S, Marcelo F, Perona A, Ruiz LB, Morreale A, Caņada FJ, Jimenez-Barbero J, Andreu JM
Abstract
FtsZ is the organizer of cell division in most bacteria and a target in the quest for new antibiotics. FtsZ is a tubulin-like...
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08-08-2015 12:17 PM
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Publication date: Available online 27 December 2014
Source:Journal of Magnetic Resonance</br>
Author(s): M.-A. Sani , F. Separovic</br>
Understanding the structure of membrane-active peptides faces many challenges associated with the development of appropriate model membrane systems as the peptide structure depends strongly on the lipid environment. This perspective provides a brief overview of the approach taken to study antimicrobial and amyloid peptides in phospholipid...
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12-28-2014 10:29 PM
[NMR paper] Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Nat Chem Biol. 2013 Mar 3;
Authors: Banci L, Barbieri L, Bertini I, Luchinat E, Secci E, Zhao Y, Aricescu AR
Abstract
We use NMR directly in live human cells to describe the complete post-translational maturation process of human superoxide dismutase 1 (SOD1). We follow, at atomic resolution, zinc binding, homodimer formation and copper uptake, and discover that copper chaperone for...
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03-05-2013 03:25 PM
[NMR paper] Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Chem Commun (Camb). 2013 Feb 26;
Authors: Takaoka Y, Kioi Y, Morito A, Otani J, Arita K, Ashihara E, Ariyoshi M, Tochio H, Shirakawa M, Hamachi I
Abstract
Here we describe how a (19)F-probe incorporated into an endogenous protein by a chemical biology method revealed protein dynamics. By explicit determination of ligand-bound and unbound structures with...
A Fluorescent Probe with Improved Water SolubilityPermits the Analysis of Protein S-DepalmitoylationActivity in Live Cells
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