Fluorescence Microscopy and Solution NMR Studies of Cytoskeletal Proteins from Tetrahymena

		BioNMR > Educational resources > Journal club
Fluorescence Microscopy and Solution NMR Studies of Cytoskeletal Proteins from Tetrahymena

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

01-28-2015, 05:28 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Fluorescence Microscopy and Solution NMR Studies of Cytoskeletal Proteins from Tetrahymena

Fluorescence Microscopy and Solution NMR Studies of Cytoskeletal Proteins from Tetrahymena

Publication date: 27 January 2015
Source:Biophysical Journal, Volume 108, Issue 2, Supplement 1

Author(s): Robert Sterner , Jerry Honts , Adina Kilpatrick

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] ?-Helical architecture of cytoskeletal bactofilin filaments revealed by solid-state NMR. ?-Helical architecture of cytoskeletal bactofilin filaments revealed by solid-state NMR. ?-Helical architecture of cytoskeletal bactofilin filaments revealed by solid-state NMR. Proc Natl Acad Sci U S A. 2014 Dec 30; Authors: Vasa S, Lin L, Shi C, Habenstein B, Riedel D, Kühn J, Thanbichler M, Lange A Abstract Bactofilins are a widespread class of bacterial filament-forming proteins, which serve as cytoskeletal scaffolds in various cellular pathways. They are characterized by a conserved architecture, featuring a central...	nmrlearner	Journal club	0	01-01-2015 11:00 PM
Interactions of gemini surfactants with two model proteins: NMR, CD, and fluorescence spectroscopies Interactions of gemini surfactants with two model proteins: NMR, CD, and fluorescence spectroscopies 1 March 2012 Publication year: 2012 Source:Journal of Colloid and Interface Science, Volume 369, Issue 1</br> </br> Gemini surfactants have two polar head groups and two hydrocarbon tails. Compared with conventional surfactants, geminis have much lower (?M vs. mM) critical micelle concentrations and possess slower (ms vs. ?s) monomer ? micelle kinetics. The structure of the gemini surfactants studied is ·2Br- where s =4, 5, or 6. Our objective is to reveal the effect...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Solution NMR studies of polytopic ?-helical membrane proteins. Solution NMR studies of polytopic ?-helical membrane proteins. Solution NMR studies of polytopic ?-helical membrane proteins. Curr Opin Struct Biol. 2011 Jul 18; Authors: Nietlispach D, Gautier A NMR spectroscopy has established itself as one of the main techniques for the structural study of integral membrane proteins. Remarkably, over the last few years, substantial progress has been achieved in the structure determination of increasingly complex polytopical ?-helical membrane proteins, with their size approaching ~100kDa. Such advances are the...	nmrlearner	Journal club	0	07-23-2011 08:54 AM
[NMR paper] Metal ion binding to calmodulin: NMR and fluorescence studies. Metal ion binding to calmodulin: NMR and fluorescence studies. Related Articles Metal ion binding to calmodulin: NMR and fluorescence studies. Biometals. 1998 Sep;11(3):213-22 Authors: Ouyang H, Vogel HJ Calmodulin is an important second messenger protein which is involved in a large variety of cellular pathways. Calmodulin is sensitive to fluctuations in the intracellular Ca2+ levels and is activated by the binding of four Ca2+ ions. In spite of the important role it plays in signal transduction pathways, it shows a surprisingly broad...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] On choosing a detergent for solution NMR studies of membrane proteins. On choosing a detergent for solution NMR studies of membrane proteins. Related Articles On choosing a detergent for solution NMR studies of membrane proteins. J Biomol NMR. 1998 May;11(4):381-6 Authors: Vinogradova O, Sönnichsen F, Sanders CR Translational diffusion coefficients and catalytic activities were measured for the integral membrane protein diacylglycerol kinase (DAGK) in a variety of types of detergent micelles. Despite the structural diversity of the detergents examined, the translational diffusion coefficients observed for DAGK...	nmrlearner	Journal club	0	11-17-2010 11:06 PM
[NMR paper] 1H NMR and fluorescence studies of the complexation of DMPG by wheat non-specific lip 1H NMR and fluorescence studies of the complexation of DMPG by wheat non-specific lipid transfer protein. Global fold of the complex. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 1H NMR and fluorescence studies of the complexation of DMPG by wheat non-specific lipid transfer protein. Global fold of the complex. FEBS Lett. 1997 Oct 20;416(2):130-4 Authors: Sodano P, Caille A, Sy D, de Person G, Marion D, Ptak M Plant non-specific lipid transfer proteins (LTPs) are...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvi Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein. Related Articles Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein. Biochemistry. 1994 Jan 11;33(1):65-73 Authors: Yamasaki K, Shirouzu M, Muto Y, Fujita-Yoshigaki J, Koide H, Ito Y, Kawai G, Hattori S, Yokoyama S, Nishimura S The Tyr residues in positions 32 and 40 of human c-Ha-Ras...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvi Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein. Related Articles Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein. Biochemistry. 1994 Jan 11;33(1):65-73 Authors: Yamasaki K, Shirouzu M, Muto Y, Fujita-Yoshigaki J, Koide H, Ito Y, Kawai G, Hattori S, Yokoyama S, Nishimura S The Tyr residues in positions 32 and 40 of human c-Ha-Ras...	nmrlearner	Journal club	0	08-22-2010 03:33 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off