Analysis of enteric microbiota function indirectly through the fecal metabolome has the potential to be an informative diagnostic tool. However, metabolomic analysis of feces is hampered by high concentrations of macromolecules such as proteins, fats, and fiber in samples. Three methods-ultrafiltration (UF), Bligh-Dyer (BD), and no extraction (samples added directly to buffer, vortexed, and centrifuged)-were tested on multiple rat (n = 10) and chicken (n = 8) fecal samples to ascertain whether...
NMR-based metabolomics: From sample preparation to applications in nutrition research
NMR-based metabolomics: From sample preparation to applications in nutrition research
Publication date: November 2014
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 83</br>
Author(s): Lorraine Brennan</br>
Metabolomics is the study of metabolites present in biological samples such as biofluids, tissue/cellular extracts and culture media. Measurement of these metabolites is achieved through use of analytical techniques such as NMR and mass spectrometry coupled to liquid chromatography. Combining metabolomic data with multivariate data analysis...
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11-06-2014 08:42 AM
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
From The DNP-NMR Blog:
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
Le, D., et al., Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers. Macromolecules, 2014: p. 140613123939001.
http://pubs.acs.org/doi/abs/10.1021/ma500788n
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06-18-2014 06:09 PM
[NMR paper] Fecal and urinary NMR-based metabolomics unveil an aging signature in mice.
Fecal and urinary NMR-based metabolomics unveil an aging signature in mice.
Related Articles Fecal and urinary NMR-based metabolomics unveil an aging signature in mice.
Exp Gerontol. 2013 Oct 29;
Authors: Calvani R, Brasili E, Praticò G, Capuani G, Tomassini A, Marini F, Sciubba F, Finamore A, Roselli M, Marzetti E, Miccheli A
Abstract
BACKGROUND: Aging is characterized by derangements in multiple metabolic pathways that progressively constrict the homeostatic reserve (homeostenosis). The signature of metabolic alterations that accompany...
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11-05-2013 06:53 PM
[NMRpipe Yahoo group] Re: baseline correction
Re: baseline correction
Ok, one more trick, hope it helps! For a given vector-based nmrPipe processing function, you can select which vectors from the data are passed to it, and which
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01-12-2013 03:01 AM
[NMRpipe Yahoo group] Re: baseline correction
Re: baseline correction
Thanks Frank! ... Not much to do about that then as bruker machines are the only ones at my disposal :) ... This actually does improve the distortions (but not
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01-12-2013 03:01 AM
[NMRpipe Yahoo group] Re: baseline correction
Re: baseline correction
Bruker needs to have good baseline correction facilities in their software because their acquistion methods result in such badly distorted baselines. That
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01-10-2013 10:43 AM
[NMRpipe Yahoo group] Re: baseline correction
Re: baseline correction
Dear Pipe users, I have the same problem as Kate: heavy baseline distortions in the acquisition dimension in the methyl region of some of my 2D and 3D noesys.
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01-08-2013 09:23 AM
[Question from NMRWiki Q&A forum] qfil: baseline correction
qfil: baseline correction
"the t1 dimensio was processed using the baseline correction mode “qfil” in theXWINNMR software"
Does anyone know about "qfil" method in XWINNMR?How can I realize the "qfil" in vnmrJ?
Thanks in advance!
Fecal (1)H-NMR Metabolomics: A Comparison of Sample Preparation Methods for NMR and Novel in Silico Baseline Correction
Linear Mode
