[NMR paper] Fate of Pup inside the Mycobacterium Proteasome Studied by in-Cell NMR.

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Fate of Pup inside the Mycobacterium Proteasome Studied by in-Cell NMR.

Related Articles Fate of Pup inside the Mycobacterium Proteasome Studied by in-Cell NMR.

PLoS One. 2013;8(9):e74576

Authors: Maldonado AY, Burz DS, Reverdatto S, Shekhtman A

Abstract
The Mycobacterium tuberculosis proteasome is required for maximum virulence and to resist killing by the host immune system. The prokaryotic ubiquitin-like protein, Pup-GGE, targets proteins for proteasome-mediated degradation. We demonstrate that Pup-GGQ, a precursor of Pup-GGE, is not a substrate for proteasomal degradation. Using STINT-NMR, an in-cell NMR technique, we studied the interactions between Pup-GGQ, mycobacterial proteasomal ATPase, Mpa, and Mtb proteasome core particle (CP) inside a living cell at amino acid residue resolution. We showed that under in-cell conditions, in the absence of the proteasome CP, Pup-GGQ interacts with Mpa only weakly, primarily through its C-terminal region. When Mpa and non-stoichiometric amounts of proteasome CP are present, both the N-terminal and C-terminal regions of Pup-GGQ bind strongly to Mpa. This suggests a mechanism by which transient binding of Mpa to the proteasome CP controls the fate of Pup.


PMID: 24040288 [PubMed - in process]



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