Publication date: Available online 21 November 2016 Source:Journal of Magnetic Resonance
Author(s): Yulan Lin, Pieter E S Smith, Zhiyong Zhang, Lucio Frydman
Multidimensional Nuclear Magnetic Resonance (NMR) provides a unique window into structure and dynamics at an atomic level. Traditionally, given the scan-by-scan time modulation involved in these experiments, the duration of nD NMR increases exponentially with spectral dimensionality. In addition, acquisition times increase as the number of spectral elements being sought in each indirect domain –given by the ratio between the spectral bandwidth being targeted and the resolution desired for solving the resonances. These long sampling times can be substantially reduced by exploiting a priori information, of the type that is often available from lower-dimensionality acquisitions. This work presents a novel approach that exploits previous 2D information to speed up the acquisition of 3D spectra, based on what we denote as a Time-Optimized FouriEr Encoding (TOFEE) of pre-targeted peaks. Such 3D TOFEE experiments, which present points in common with Hadamard-encoded 3D acquisitions, do not necessarily require more scans than their 2D counterparts. This is here demonstrated based on extensions of 2D Heteronuclear Single-quantum Coherence (HSQC) experiments, to 3D HSQC-TOCSY and 3D HSQC-NOESY acquisitions. The theoretical basis of this new approach is given, and experimental demonstrations are presented on small molecule and protein-based model systems. Graphical abstract
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[Question from NMRWiki Q&A forum] Looking for hsqc-based ZZ exchange bruker pulse program
Looking for hsqc-based ZZ exchange bruker pulse program
hi nmrwikier !
Anyone know if the hsqc-based zz exchange pulse prog developed by Farrow et al. (1994) is available on standart bruker pulse prog library and what is its name ?
Many thanks !
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Go to BMNRC community to find more info about this topic.
A fast approach to 3D HSQC-based spectroscopy based on a phase-based Fourier encoding of pre-targeted resonances
Linear Mode
