FacilitatedAssignment of Large Protein NMR Signalswith Covariance Sequential Spectra Using Spectral Derivatives

		BioNMR > Educational resources > Journal club
FacilitatedAssignment of Large Protein NMR Signalswith Covariance Sequential Spectra Using Spectral Derivatives

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-16-2014, 08:38 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

FacilitatedAssignment of Large Protein NMR Signalswith Covariance Sequential Spectra Using Spectral Derivatives

FacilitatedAssignment of Large Protein NMR Signalswith Covariance Sequential Spectra Using Spectral Derivatives

Bradley J. Harden, Scott R. Nichols and Dominique P. Frueh

Journal of the American Chemical Society
DOI: 10.1021/ja5058407

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Sequential protein NMR assignments in the liquid state via sequential data acquisition. Sequential protein NMR assignments in the liquid state via sequential data acquisition. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Sequential protein NMR assignments in the liquid state via sequential data acquisition. J Magn Reson. 2013 Dec 14;239C:23-28 Authors: Wiedemann C, Bellstedt P, Kirschstein A, Häfner S, Herbst C, Görlach M, Ramachandran R Abstract Two different NMR pulse schemes involving sequential (1)H data acquisition are...	nmrlearner	Journal club	0	01-04-2014 01:39 AM
[NMR paper] Sequential protein NMR assignments in the liquid state via sequential data acquisition Sequential protein NMR assignments in the liquid state via sequential data acquisition Publication date: Available online 14 December 2013 Source:Journal of Magnetic Resonance</br> Author(s): Christoph Wiedemann , Peter Bellstedt , Anika Kirschstein , Sabine Häfner , Christian Herbst , Matthias Görlach , Ramadurai Ramachandran</br> Two different NMR pulse schemes involving sequential 1H data acquisition are presented for achieving protein backbone sequential resonance assignments: (i) acquisition of 3D {HCCNH & HNCACONH} and (ii) collection of 3D {HNCOCANH &...	nmrlearner	Journal club	0	12-14-2013 04:48 PM
[NMR paper] Permeation through Phospholipid Bilayers, Skin-Cell Penetration, Plasma Stability, and CD Spectra of ?- and ?-Oligoproline Derivatives. Permeation through Phospholipid Bilayers, Skin-Cell Penetration, Plasma Stability, and CD Spectra of ?- and ?-Oligoproline Derivatives. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Permeation through Phospholipid Bilayers, Skin-Cell Penetration, Plasma Stability, and CD Spectra of ?- and ?-Oligoproline Derivatives. Chem Biodivers. 2013 Jan;10(1):1-38 Authors: Kolesinska B, Podwysocka DJ, Rueping MA, Seebach D, Kamena F, Walde P, Sauer M, Windschiegl B,...	nmrlearner	Journal club	0	02-03-2013 10:19 AM
Spectral editing of two-dimensional magic-angle-spinning solid-state NMR spectra for protein resonance assignment and structure determination Spectral editing of two-dimensional magic-angle-spinning solid-state NMR spectra for protein resonance assignment and structure determination Abstract Several techniques for spectral editing of 2D 13Câ??13C correlation NMR of proteins are introduced. They greatly reduce the spectral overlap for five common amino acid types, thus simplifying spectral assignment and conformational analysis. The carboxyl (COO) signals of glutamate and aspartate are selected by suppressing the overlapping amide Nâ??CO peaks through 13Câ??15N dipolar dephasing. The sidechain methine (CH) signals of valine,...	nmrlearner	Journal club	0	10-13-2012 04:42 AM
[NMRpipe Yahoo group] Re: extracting spectral regions from 2D spectra Re: extracting spectral regions from 2D spectra Many thanks to Nicholas and Frank. I tried out the steps posted by Frank and they worked perfectly fine with me. By applying minor variations, I could get the More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-16-2011 08:22 AM
[NMRpipe Yahoo group] Re: extracting spectral regions from 2D spectra Re: extracting spectral regions from 2D spectra Hi Aswani, I was thinking that one way to solve your problem may be to turn your spectrum into a pseudo 3D using something like series.com. Then, you may be More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-16-2011 08:22 AM
[NMRpipe Yahoo group] Re: extracting spectral regions from 2D spectra Re: extracting spectral regions from 2D spectra Many thanks to Nicholas and Frank. I tried out the steps posted by Frank and they worked perfectly fine with me. By applying minor variations, I could get the More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-12-2011 07:41 PM
[NMR paper] Solid-state dipolar INADEQUATE NMR spectroscopy with a large double-quantum spectral Solid-state dipolar INADEQUATE NMR spectroscopy with a large double-quantum spectral width. Related Articles Solid-state dipolar INADEQUATE NMR spectroscopy with a large double-quantum spectral width. J Magn Reson. 1999 Jan;136(1):86-91 Authors: Hong M A technique for obtaining dipolar-mediated INADEQUATE NMR spectra with a large spectral window in the double-quantum dimension is presented. Using the dipolar recoupling sequence C7 to excite the double-quantum coherence under magic-angle spinning, the technique involves incrementing the...	nmrlearner	Journal club	0	11-18-2010 07:05 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off