Background/Objectives: Metabolic profiling of tissue samples via liquid-state nuclear magnetic resonance (NMR) requires the extraction of polar metabolites in a suitable deuterated solvent. Such methods often prioritise metabolite recovery over protein removal due to the relatively low sensitivity of NMR metabolomics and the routine use of methods able to supress residual protein signals. However, residual protein may impact metabolite integrity and the metabolite stability after NMR sample...
[NMR paper] NMR Precision Metabolomics: Dynamic Peak Sum Thresholding and Navigators for Highly Standardized and Reproducible Metabolite Profiling of Clinical Urine Samples
NMR Precision Metabolomics: Dynamic Peak Sum Thresholding and Navigators for Highly Standardized and Reproducible Metabolite Profiling of Clinical Urine Samples
Metabolomics, especially urine-based studies, offers incredible promise for the discovery and development of clinically impactful biomarkers. However, due to the unique challenges of urine, a highly precise and reproducible workflow for NMR-based urine metabolomics is lacking. Using 1D and 2D non-uniform sampled (NUS) ¹H-^(13)C NMR spectroscopy, we systematically explored how changes in hydration or specific gravity (SG) and pH can...
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05-24-2024 04:50 PM
[NMR paper] The effect of liquid-liquid extraction on metabolite detection and analysis using NMR spectroscopy in human synovial fluid
The effect of liquid-liquid extraction on metabolite detection and analysis using NMR spectroscopy in human synovial fluid
The evaluation of joint disease using synovial fluid is an emerging field of metabolic profiling. The analysis is challenged by multiple macromolecules which can obscure the small molecule chemistry. The use of protein precipitation and extraction has been evaluated previously, but not in synovial fluid. We systematically review the published NMR spectroscopy methods of synovial fluid analysis and investigated the efficacy of three different protein precipitation...
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01-27-2023 05:53 PM
[NMR paper] NMR-based metabolomics reveals the metabolite profiles of Vibrio parahaemolyticus under ferric iron stimulation.
NMR-based metabolomics reveals the metabolite profiles of Vibrio parahaemolyticus under ferric iron stimulation.
Related Articles NMR-based metabolomics reveals the metabolite profiles of Vibrio parahaemolyticus under ferric iron stimulation.
J Microbiol. 2017 Aug;55(8):628-634
Authors: Zhou J, Lu C, Zhang D, Ma C, Su X
Abstract
Vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. In our previous research, the protein expression of V....
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07-29-2017 10:35 AM
[NMR paper] Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Biochemistry. 2016 Apr 11;
Authors: Nikolaev YV, Kochanowski K, Link H, Sauer U, Allain FH
Abstract
Protein-metabolite interactions play a vital role in the regulation of numerous cellular processes. Consequently, identifying such interactions is a key prerequisite for...
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04-12-2016 09:12 PM
[NMR paper] NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
Biochem Biophys Res Commun. 2015 Dec 1;
Authors: Wu J, Domellöf M, Zivkovic AM, Larsson G, Öhman A, Nording ML
Abstract
Low-molecular-weight metabolites in human milk are gaining increasing interest in studies of infant nutrition. In the present study, the milk metabolome...
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12-15-2015 08:09 PM
[NMR paper] Human plasma stability during handling and storage: impact on NMR metabolomics.
Human plasma stability during handling and storage: impact on NMR metabolomics.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Human plasma stability during handling and storage: impact on NMR metabolomics.
Analyst. 2014 Mar 7;139(5):1168-77
Authors: Pinto J, Domingues MR, Galhano E, Pita C, Almeida Mdo C, Carreira IM, Gil AM
Abstract
This work contributes to fill in some existing gaps in the knowledge of human plasma degradability...
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08-29-2014 05:36 PM
[NMR images] Methods in Protein Structure and Stability Analysis: NMR and EPR ...
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8/04/2014 7:46:05 AM GMT
Methods in Protein Structure and Stability Analysis: NMR and EPR ...
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04-08-2014 07:45 AM
Thermal stability of chicken brain α-spectrin repeat 17: a spectroscopic study
Thermal stability of chicken brain α-spectrin repeat 17: a spectroscopic study
Abstract Spectrin is a rod-like multi-modular protein that is mainly composed of triple-helical repeats. These repeats show very similar 3D-structures but variable conformational and thermodynamical stabilities, which may be of great importance for the flexibility and dynamic behaviour of spectrin in the cell. For instance, repeat 17 (R17) of the chicken brain spectrin α-chain is four times less stable than neighbouring repeat 16 (R16) in terms of ∆G. The structure of spectrin repeats has mainly been...
Extraction Methods for Brain Biopsy NMR Metabolomics: Balancing Metabolite Stability and Protein Precipitation
Linear Mode
