NMR paper The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile 1Hs of SARS-CoV-2-derived RNAs

		BioNMR > Educational resources > Journal club
[NMR paper] The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile 1Hs of SARS-CoV-2-derived RNAs

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-31-2021, 08:07 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile 1Hs of SARS-CoV-2-derived RNAs

The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile 1Hs of SARS-CoV-2-derived RNAs

Hadamard encoded saturation transfer can significantly improve the efficiency of NOE-based NMR correlations from labile protons in proteins, glycans and RNAs, increasing the sensitivity of cross-peaks by an order of magnitude and shortening experimental times by >=100-fold. These schemes, however, fail when tackling correlations within a pool of labile protons -for instance imino-imino correlations in RNAs or amide-amide correlations in proteins. Here we analyze the origin of the artifacts...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Heteronuclear transfers from labile protons in biomolecular NMR: Cross polarization, revisited Heteronuclear transfers from labile protons in biomolecular NMR: Cross polarization, revisited INEPT- and HMQC-based pulse sequences are widely used to transfer polarization between heteronuclei, particularly in biomolecular spectroscopy: they are easy to setup and involve low power deposition. Still, these short-pulse polarization transfers schemes are challenged by fast solvent chemical exchange. An alternative to improve these heteronuclear transfers is J-driven cross polarization (J-CP), which transfers polarization by spin-locking the coupled spins under Hartmann-Hahn conditions.... ...	nmrlearner	Journal club	0	10-25-2021 10:32 PM
[ASAP] The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited Mihajlo Novakovic, Marcos D. Battistel, Hugo F. Azurmendi, Maria-Grazia Concilio, Daro?n I. Freedberg, and Lucio Frydman https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c04512/20210604/images/medium/ja1c04512_0008.gif Journal of the American Chemical Society DOI: 10.1021/jacs.1c04512 http://feeds.feedburner.com/~r/acs/jacsat/~4/H2MJ7chRt4g	nmrlearner	Journal club	0	06-05-2021 09:30 AM
[NMR paper] The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited Glycan structures are often stabilized by a repertoire of hydrogen-bonded donor/acceptor groups, revealing longer-lived structures that could represent biologically relevant conformations. NMR provides unique data on these hydrogen-bonded networks from multidimensional experiments detecting cross-peaks resulting from through-bond (TOCSY) or through-space (NOESY) interactions. However, fast OH/H(2)O exchange, and the spectral proximity among these NMR resonances, hamper the use of glycans'...	nmrlearner	Journal club	0	06-05-2021 09:30 AM
[NMR paper] Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids. Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.nature.com-images-npg_logo.gif Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids. Nat Commun. 2020 10 21;11(1):5317 Authors: Novakovic M,...	nmrlearner	Journal club	0	10-24-2020 10:43 PM
Sensitivity-enhanced detection of non-labile proton and carbon NMR spectra on water resonances #DNPNMR From The DNP-NMR Blog: Sensitivity-enhanced detection of non-labile proton and carbon NMR spectra on water resonances #DNPNMR Novakovic, M., et al., Sensitivity-enhanced detection of non-labile proton and carbon NMR spectra on water resonances. Phys. Chem. Chem. Phys., 2017. 20(1): p. 56-62. https://www.ncbi.nlm.nih.gov/pubmed/29171604	nmrlearner	News from NMR blogs	0	02-19-2018 08:46 PM
[Question from NMRWiki Q&A forum] OH, NH, SH Labile protons in water nmr OH, NH, SH Labile protons in water nmr Hello all, Just to mirror Craig Grimmer's question about labile protons in non-aqueous solvents, does anyone know of any good articles dealing with labile proton behavior in water? Thanks,	nmrlearner	News from other NMR forums	0	03-11-2015 09:59 PM
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments From The DNP-NMR Blog: Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments Takahashi, H., et al., Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments. J Magn Reson, 2013. 239C(0): p. 91-99. http://www.ncbi.nlm.nih.gov/pubmed/24480716	nmrlearner	News from NMR blogs	0	02-10-2014 08:46 PM
Labile proton assignment in DNA can anybody help in assignment of exchangeable proton in DNA? I got stuck in assigning the labile proton in my DNA sequence. can anyone provide me the strategy of Labile proton assignment in DNA. I would be highly thankful for. thanks	spbiotech08	NMR Questions and Answers	0	06-04-2013 02:42 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off