Expression and purification of myristoylated matrix protein of Mason-Pfizer monkey virus for NMR and MS measurements.
Protein Expr Purif. 2011 May 24;
Authors: Prchal J, Junkova P, Strmiskova M, Lipov J, Hynek R, Ruml T, Hrabal R
Matrix proteins play multiple roles both in early and late stages of the viral replication cycle. Their N-terminal myristoylation is important for interaction with the host cell membrane during virus budding. We used Escherichia coli, carrying N-myristoyltransferase gene, for the expression of the myristoylated His-tagged matrix protein of Mason-Pfizer monkey virus. An efficient, single-step purification procedure eliminating all contaminating proteins including, importantly, the non-myristoylated matrix protein was designed. The comparison of NMR spectra of matrix protein with its myristoylated form revealed substantial structural changes induced by this fatty acid modification.
PMID: 21640189 [PubMed - as supplied by publisher]
Expression, purification, and reconstitution of the transmembrane domain of the human amyloid precursor protein for NMR studies.
Expression, purification, and reconstitution of the transmembrane domain of the human amyloid precursor protein for NMR studies.
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Abstract
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02-16-2011 07:40 PM
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Expression and purification of myristoylated matrix protein of Mason-Pfizer monkey virus for NMR and MS measurements.
Linear Mode
