This study utilizes the advanced analytical capabilities of nuclear magnetic resonance metabolomics to perform an untargeted and comprehensive analysis of the metabolic profile ofChrysochromulina rotalis under varying environmental conditions and photobioreactor setups. By identifying key biomolecules, including lipids, pigments, proteins, and carbohydrates, this research highlights the remarkable adaptability ofC. rotalis and its potential as a valuable source of bioactive compounds with...
[NMR paper] Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies
Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies
The use of Escherichia coli for recombinant protein production is a cornerstone in structural biology, particularly for nuclear magnetic resonance (NMR) spectroscopy studies. Understanding the metabolic behavior of E. coli under different carbon sources is critical for optimizing isotope labeling strategies, which are essential for protein structure determination by NMR. Recent advancements, such as mixed pyruvate labeling, have enabled improved backbone resonance assignment...
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03-07-2025 04:49 AM
Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies
Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies
The use of Escherichia coli for recombinant protein production is a cornerstone in structural biology, particularly for nuclear magnetic resonance (NMR) spectroscopy studies. Understanding the metabolic behavior of E. coli under different carbon sources is critical for optimizing isotope labeling strategies, which are essential for protein structure determination by NMR. Recent advancements, such as mixed pyruvate labeling, have enabled improved backbone resonance assignment...
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03-06-2025 04:47 PM
[NMR paper] Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
Related Articles Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
J Pharm Biomed Anal. 2017 Sep 12;146:324-328
Authors: Maria RM, Altei WF, Selistre-de-Araujo HS, Colnago LA
Abstract
Doxorubicin, cisplatin, and tamoxifen are part of many chemotherapeutic...
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09-16-2017 09:58 PM
[NMR paper] Profiling sulfation/epimerization pattern of full-length heparan sulfate by NMR following cell culture 13C-glucose metabolic labeling.
Profiling sulfation/epimerization pattern of full-length heparan sulfate by NMR following cell culture 13C-glucose metabolic labeling.
Related Articles Profiling sulfation/epimerization pattern of full-length heparan sulfate by NMR following cell culture 13C-glucose metabolic labeling.
Glycobiology. 2014 Oct 21;
Authors: Pegeot M, Sadir R, Eriksson I, Kjellen L, Simorre JP, Gans P, Lortat-Jacob H
Abstract
Through its ability to interact with proteins, heparan sulfate (HS) fulfills a large variety of functions. Protein binding...
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10-23-2014 05:27 PM
[NMR paper] NMR methods for metabolomics of Mammalian cell culture bioreactors.
NMR methods for metabolomics of Mammalian cell culture bioreactors.
Related Articles NMR methods for metabolomics of Mammalian cell culture bioreactors.
Methods Mol Biol. 2014;1104:223-36
Authors: Aranibar N, Reily MD
Abstract
Metabolomics has become an important tool for measuring pools of small molecules in mammalian cell cultures expressing therapeutic proteins. NMR spectroscopy has played an important role, largely because it requires minimal sample preparation, does not require chromatographic separation, and is quantitative. The...
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12-04-2013 03:12 PM
[NMR paper] 1H NMR study on the short- and long-term impact of two training programs of sprint running on the metabolic fingerprint of human serum.
1H NMR study on the short- and long-term impact of two training programs of sprint running on the metabolic fingerprint of human serum.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles 1H NMR study on the short- and long-term impact of two training programs of sprint running on the metabolic fingerprint of human serum.
J Proteome Res. 2013 Jan 4;12(1):470-80
Authors: Pechlivanis A, Kostidis S, Saraslanidis P, Petridou A, Tsalis G, Veselkov K, Mikros E, Mougios V, Theodoridis GA...
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The impact of prenatal disorders on the metabolic profile of 2nd trimester amniotic f
THE IMPACT OF PRENATAL DISORDERS ON THE METABOLIC PROFILE OF 2ND TRIMESTER AMNIOTIC FLUID: A NUCLEAR MAGNETIC RESONANCE (NMR) METABONOMIC STUDY.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles THE IMPACT OF PRENATAL DISORDERS ON THE METABOLIC PROFILE OF 2ND TRIMESTER AMNIOTIC FLUID: A NUCLEAR MAGNETIC RESONANCE (NMR) METABONOMIC STUDY.
J Proteome Res. 2010 Sep 17;
Authors: Graça G, Duarte IF, Barros AS, Goodfellow BJ, Diaz S, Pinto J, Carreira I, Galhano E, Pita C, Gil AM
This paper...
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[NMR paper] NMR strategy for determining Xaa-Pro peptide bond configurations in proteins: mutants
NMR strategy for determining Xaa-Pro peptide bond configurations in proteins: mutants of staphylococcal nuclease with altered configuration at proline-117.
Related Articles NMR strategy for determining Xaa-Pro peptide bond configurations in proteins: mutants of staphylococcal nuclease with altered configuration at proline-117.
Biochemistry. 1993 Nov 9;32(44):11810-8
Authors: Hinck AP, Eberhardt ES, Markley JL
A general approach has been developed for configurational analysis (cis or trans) of Xaa-Pro peptide bonds in proteins. This approach,...
Exploring the NMR Metabolic Landscape of Chrysochromulina rotalis: The Impact of Photobioreactor Configurations and Culture Conditions
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