Nuclear Magnetic Resonance (NMR) has been widely employed to assess diverse features of glycan-protein molecular recognition events. Different types of qualitative and quantitative information at different degrees of resolution and complexity can be extracted from the proper application of the available NMR-techniques. In fact, affinity, structural, kinetic, conformational, and dynamic characteristics of the binding process are available. Nevertheless, except in particular cases, the affinity of...
[NMR paper] Investigating protein-excipient interactions of a multivalent VHH therapeutic protein using NMR spectroscopy
Investigating protein-excipient interactions of a multivalent VHH therapeutic protein using NMR spectroscopy
Fab Fragment antigen-binding; Fc Fragment crystallizable; HMW High molecular weight; ?HMW Difference between HMW species at stress temperature and 5°C controls; IgG Immunoglobulin G; mAbs Monoclonal antibodies; MV-V(HH) Multivalent V(HH) molecule with the format aC-L(1)-aC-L(1)-aD; NMR Nuclear magnetic resonance; scFv Single-chain fragment variable; SEC Size-exclusion chromatography; V(HH) Variable domain of Heavy chain of Heavy chain-only antibody.
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09-28-2022 06:29 AM
[NMR paper] The Use of NMR to Study Transient Carbohydrate-Protein Interactions.
The Use of NMR to Study Transient Carbohydrate-Protein Interactions.
Related Articles The Use of NMR to Study Transient Carbohydrate-Protein Interactions.
Front Mol Biosci. 2018;5:33
Authors: Nieto PM
Abstract
Carbohydrates are biologically ubiquitous and are essential to the existence of all known living organisms. Although they are better known for their role as energy sources (glucose/glycogen or starch) or structural elements (chitin or cellulose), carbohydrates also participate in the recognition events of molecular...
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04-27-2018 10:26 PM
[NMR paper] Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy.
Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy.
Related Articles Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy.
Methods Mol Biol. 2017;1588:143-156
Authors: Grondin JM, Langelaan DN, Smith SP
Abstract
Solution-state nuclear magnetic resonance (NMR) spectroscopy can be used to monitor protein-carbohydrate interactions. Two-dimensional (1)H-(15)N heteronuclear single quantum coherence (HSQC)-based techniques described in this chapter can be used quickly and effectively to...
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04-19-2017 01:07 PM
[NMR paper] Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Biopolymers. 2013 Jun 20;
Authors: Bewley CA, Shahzad-Ul-Hussan S
Abstract
Interactions between proteins and soluble carbohydrates and/or surface displayed glycans are central to countless recognition, attachment and signaling events in biology. The physical chemical features associated with these binding events vary considerably, depending on the biological system of interest. For example, carbohydrate-protein...
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06-21-2013 01:10 PM
Carbohydrate-Protein Interactions: A 3D View by NMR.
Carbohydrate-Protein Interactions: A 3D View by NMR.
Carbohydrate-Protein Interactions: A 3D View by NMR.
Chembiochem. 2011 Apr 15;
Authors: Roldós V, Cañada FJ, Jiménez-Barbero J
This review focuses on the application of NMR methods for understanding, at the molecular and atomic levels, the diverse mechanisms by which sugar molecules are recognised by the binding sites of lectins, antibodies and enzymes. Given the intrinsic chemical natures of sugars and their flexibility, it is well established that NMR parameters should be complemented by...
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04-19-2011 11:01 PM
[NMR paper] New structural insights into carbohydrate-protein interactions from NMR spectroscopy.
New structural insights into carbohydrate-protein interactions from NMR spectroscopy.
Related Articles New structural insights into carbohydrate-protein interactions from NMR spectroscopy.
Curr Opin Struct Biol. 2003 Oct;13(5):646-53
Authors: Kogelberg H, Solís D, Jiménez-Barbero J
Recently developed NMR methods have been applied to discover carbohydrate ligands for proteins and to identify their binding epitopes. The structural details of carbohydrate-protein complexes have also been examined by NMR, providing site-specific information on the...
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11-24-2010 09:16 PM
[NMR paper] NMR investigations of protein-carbohydrate interactions: insights into the topology o
NMR investigations of protein-carbohydrate interactions: insights into the topology of the bound conformation of a lactose isomer and beta-galactosyl xyloses to mistletoe lectin and galectin-1.
Related Articles NMR investigations of protein-carbohydrate interactions: insights into the topology of the bound conformation of a lactose isomer and beta-galactosyl xyloses to mistletoe lectin and galectin-1.
Biochim Biophys Acta. 2001 Dec 19;1568(3):225-36
Authors: Alonso-Plaza JM, Canales MA, Jiménez M, Roldán JL, García-Herrero A, Iturrino L, Asensio JL,...
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11-19-2010 08:44 PM
[NMR paper] NMR investigations of protein-carbohydrate interactions: studies on the relevance of
NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex between pseudohevein and N,N',N"-triacetylchitotriose.
Related Articles NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex...
Exploring multivalent carbohydrate-protein interactions by NMR
Linear Mode
