Exploring Folding Cooperativity of a Repeat Protein Folding by 2D-NMR Detected Pressure Perturbation

		BioNMR > Educational resources > Journal club
Exploring Folding Cooperativity of a Repeat Protein Folding by 2D-NMR Detected Pressure Perturbation

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-17-2016, 07:50 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Exploring Folding Cooperativity of a Repeat Protein Folding by 2D-NMR Detected Pressure Perturbation

Exploring Folding Cooperativity of a Repeat Protein Folding by 2D-NMR Detected Pressure Perturbation

Publication date: 16 February 2016
Source:Biophysical Journal, Volume 110, Issue 3, Supplement 1

Author(s): Martin J. Fossat, Angel Garcia, Doug Barrick, Christian Roumestand, Catherine A. Royer

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] The Energetics of a Three-State Protein Folding System Probed by High-Pressure Relaxation Dispersion NMR Spectroscopy. The Energetics of a Three-State Protein Folding System Probed by High-Pressure Relaxation Dispersion NMR Spectroscopy. Related Articles The Energetics of a Three-State Protein Folding System Probed by High-Pressure Relaxation Dispersion NMR Spectroscopy. Angew Chem Int Ed Engl. 2015 Sep 14;54(38):11157-11161 Authors: Tugarinov V, Libich DS, Meyer V, Roche J, Clore GM Abstract The energetic and volumetric properties of a three-state protein folding system, comprising a metastable triple mutant of the Fyn SH3 domain, have been...	nmrlearner	Journal club	0	09-10-2015 02:01 PM
[NMR paper] Exploring the Protein Folding Pathway with High-Pressure NMR: Steady-State and Kinetics Studies. Exploring the Protein Folding Pathway with High-Pressure NMR: Steady-State and Kinetics Studies. Related Articles Exploring the Protein Folding Pathway with High-Pressure NMR: Steady-State and Kinetics Studies. Subcell Biochem. 2015;72:261-278 Authors: Roche J, Dellarole M, Royer CA, Roumestand C Abstract Defining the physical-chemical determinants of protein folding and stability, under normal and pathological conditions has constituted a major subfield in biophysical chemistry for over 50 years. Although a great deal of...	nmrlearner	Journal club	0	07-16-2015 11:21 AM
[NMR images] ... equilibrium folding intermediate of an archaeal ankyrin repeat protein http://www.pnas.org/content/105/10/3779/F6.large.jpg 7/06/2014 7:13:57 PM GMT ... equilibrium folding intermediate of an archaeal ankyrin repeat protein More...	nmrlearner	NMR pictures	0	06-07-2014 07:12 PM
[NMR images] in vitro protein folding http://employees.csbsju.edu/hjakubowski/classes/ch331/protstructure/one-slice-landscape.jpg 20/03/2014 12:45:23 PM GMT in vitro protein folding More...	nmrlearner	NMR pictures	0	03-20-2014 12:44 PM
Effect of Internal Cavities on Folding Rates and RoutesRevealed by Real-Time Pressure-Jump NMR Spectroscopy Effect of Internal Cavities on Folding Rates and RoutesRevealed by Real-Time Pressure-Jump NMR Spectroscopy Julien Roche, Mariano Dellarole, Jose? A. Caro, Douglas R. Norberto, Angel E. Garcia, Bertrand Garcia-Moreno, Christian Roumestand and Catherine A. Royer http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja406682e/aop/images/medium/ja-2013-06682e_0007.gif Journal of the American Chemical Society DOI: 10.1021/ja406682e http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	09-19-2013 02:19 PM
[NMR paper] Effect of Internal Cavities on Folding Rates and Routes Revealed by Real-time Pressure-Jump NMR Spectroscopy. Effect of Internal Cavities on Folding Rates and Routes Revealed by Real-time Pressure-Jump NMR Spectroscopy. Effect of Internal Cavities on Folding Rates and Routes Revealed by Real-time Pressure-Jump NMR Spectroscopy. J Am Chem Soc. 2013 Aug 30; Authors: Roche J, Dellarole M, Caro JA, Norberto DR, Garcia AE, Garcia-Moreno E B, Roumestand C, Royer CA Abstract The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump...	nmrlearner	Journal club	0	08-31-2013 06:56 PM
[NMR paper] High-pressure NMR spectroscopy for characterizing folding intermediates and denatured High-pressure NMR spectroscopy for characterizing folding intermediates and denatured states of proteins. Related Articles High-pressure NMR spectroscopy for characterizing folding intermediates and denatured states of proteins. Methods. 2004 Sep;34(1):133-43 Authors: Kamatari YO, Kitahara R, Yamada H, Yokoyama S, Akasaka K Extensive structural studies using high-pressure NMR spectroscopy have recently been carried out on proteins, which potentially contribute to our understanding of the mechanisms of protein folding. Pressure shifts the...	nmrlearner	Journal club	0	11-24-2010 10:01 PM
[NMR paper] Cooperativity of a protein folding reaction probed at multiple chain positions by rea Cooperativity of a protein folding reaction probed at multiple chain positions by real-time 2D NMR spectroscopy. Related Articles Cooperativity of a protein folding reaction probed at multiple chain positions by real-time 2D NMR spectroscopy. Biochemistry. 2000 Jul 11;39(27):7910-9 Authors: Steegborn C, Schneider-Hassloff H, Zeeb M, Balbach J The refolding reaction of S54G/P55N ribonuclease T1 is a two-step process, where fast formation of a partly folded intermediate is followed by the slow reaction to the native state, limited by a trans -->...	nmrlearner	Journal club	0	11-19-2010 08:29 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off