[NMR paper] Exploring a DNA Sequence for the Three-Dimensional Structure Determination of a Silver(I)-Mediated C-C Base Pair in a DNA Duplex By (1)H NMR Spectroscopy.
Exploring a DNA Sequence for the Three-Dimensional Structure Determination of a Silver(I)-Mediated C-C Base Pair in a DNA Duplex By (1)H NMR Spectroscopy.
Related ArticlesExploring a DNA Sequence for the Three-Dimensional Structure Determination of a Silver(I)-Mediated C-C Base Pair in a DNA Duplex By (1)H NMR Spectroscopy.
Nucleosides Nucleotides Nucleic Acids. 2015 Nov 17;:1-24
Authors: Dairaku T, Furuita K, Sato H, Kondo Y, Kojima C, Ono A, Tanaka Y
Abstract
Recently, we discovered novel silver(I)-mediated cytosine-cytosine base pair (C-Ag(I)-C) in DNA duplexes. To understand the properties of these base pairs, we searched for a DNA sequence that can be used in NMR structure determination. After extensive sequence optimizations, a non-symmetric 15-base-paired DNA duplex with a single C-Ag(I)-C base pair flanked by 14 A-T base pairs was selected. In spite of its challenging length for NMR measurements (30 independent residues) with small sequence variation, we could assign most non-exchangeable protons (254 out of 270) and imino protons for structure determination.
PMID: 26576739 [PubMed - as supplied by publisher]
[NMR paper] Sequence-specific 1H-NMR assignment and determination of the secondary structure of hainantoxin-III from the spider Ornithoctonus hainana.
Sequence-specific 1H-NMR assignment and determination of the secondary structure of hainantoxin-III from the spider Ornithoctonus hainana.
Related Articles Sequence-specific 1H-NMR assignment and determination of the secondary structure of hainantoxin-III from the spider Ornithoctonus hainana.
Protein Pept Lett. 2013 Jul 1;20(7):761-6
Authors: Liu Z, Zhu Q, Hu W, Liang S
Abstract
Hainantoxin-III (HNTX-III) purified from the venom of the spider Ornithoctonus hainana is a novel neurotoxin preferentially inhibiting tetrodotoxin-sensitive...
nmrlearner
Journal club
0
02-05-2014 06:08 PM
Exploring the trigger sequence of the GCN4 coiled-coil: biased molecular dynamics resolves apparent inconsistencies in NMR measurements.
Exploring the trigger sequence of the GCN4 coiled-coil: biased molecular dynamics resolves apparent inconsistencies in NMR measurements.
Exploring the trigger sequence of the GCN4 coiled-coil: biased molecular dynamics resolves apparent inconsistencies in NMR measurements.
Protein Sci. 2010 Dec;19(12):2462-74
Authors: Missimer JH, Dolenc J, Steinmetz MO, van Gunsteren WF
Trigger sequences are indispensable elements for coiled-coil formation. The monomeric helical trigger sequence of the yeast transcriptional activator GCN4 has been investigated...
nmrlearner
Journal club
0
03-13-2011 04:01 AM
[NMR paper] NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by
NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.
Related Articles NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14147-51
Authors: Pervushin K, Ono A, Fernández C, Szyperski T, Kainosho M, Wüthrich K
This paper describes the NMR observation of 15N---15N and 1H---15N scalar couplings across the hydrogen bonds in Watson-Crick base pairs...
nmrlearner
Journal club
0
11-17-2010 11:15 PM
[NMR paper] NMR determination of the secondary structure and the three-dimensional polypeptide ba
NMR determination of the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR determination of the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2.
FEBS Lett. 1993 Nov 29;335(1):18-26
Authors: Szyperski T, Scheek S, Johansson J, Assmann G, Seedorf U, Wüthrich K
Nuclear magnetic resonance (NMR)...
nmrlearner
Journal club
0
08-22-2010 03:01 AM
[NMR paper] Sequence-specific 1H-NMR assignment and determination of the secondary structure of b
Sequence-specific 1H-NMR assignment and determination of the secondary structure of bovine heart fatty-acid-binding protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Sequence-specific 1H-NMR assignment and determination of the secondary structure of bovine heart fatty-acid-binding protein.
Eur J Biochem. 1992 Dec 15;210(3):901-10
Authors: Lücke C, Lassen D, Kreienkamp HJ, Spener F, Rüterjans H
The nearly complete...
nmrlearner
Journal club
0
08-21-2010 11:45 PM
[NMR paper] Determination of the three-dimensional solution structure of the histidine-containing
Determination of the three-dimensional solution structure of the histidine-containing phosphocarrier protein HPr from Escherichia coli using multidimensional NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Determination of the three-dimensional solution structure of the histidine-containing phosphocarrier protein HPr from Escherichia coli using multidimensional NMR spectroscopy.
Eur J Biochem. 1992 Dec 15;210(3):881-91
...
[NMR paper] Decreased imino proton exchange and base-pair opening in the IHF-DNA complex measured
Decreased imino proton exchange and base-pair opening in the IHF-DNA complex measured by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Decreased imino proton exchange and base-pair opening in the IHF-DNA complex measured by NMR.
J Mol Biol. 1999 May 14;288(4):659-71
Authors: Dhavan GM, Lapham J, Yang S, Crothers DM
Integration Host Factor, IHF, is an E. coli DNA binding protein that imposes a substantial bend on DNA. Previous footprinting studies and bending...
Exploring a DNA Sequence for the Three-Dimensional Structure Determination of a Silver(I)-Mediated C-C Base Pair in a DNA Duplex By (1)H NMR Spectroscopy.
Linear Mode
