Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies
 

Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies

The use of Escherichia coli for recombinant protein production is a cornerstone in structural biology, particularly for nuclear magnetic resonance (NMR) spectroscopy studies. Understanding the metabolic behavior of E. coli under different carbon sources is critical for optimizing isotope labeling strategies, which are essential for protein structure determination by NMR. Recent advancements, such as mixed pyruvate labeling, have enabled improved backbone resonance assignment in large proteins,...

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