Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy

		BioNMR > Educational resources > Journal club
Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-14-2014, 12:53 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy

Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy

Publication date: Available online 13 June 2014
Source:Polymer

Author(s): Dian Xu , Jeffery L. Yarger , Gregory P. Holland

Spider dragline silk is an outstanding biopolymer with a strength that exceeds steel by weight and a toughness greater than high-performance fibers like Kevlar. For this reason, understanding how a spider converts the gel-like, aqueous protein spinning dope within the major ampullate (MA) gland into a super fiber is of great importance for developing future biomaterials based on spider silk. In this work, the initial state of the silk proteins within Black Widow MA glands was probed with solution-state NMR spectroscopy. 15N relaxation parameters, T1, T2 and 15N-{1H} steady-state NOE were measured for twelve backbone environments at two spectrometer frequencies, 500 and 800 MHz. The NMR relaxation parameters extracted for all twelve environments are consistent with MA silk protein backbone dynamics on the fast sub-nanosecond timescale. Therefore, it is concluded that the repetitive core of spider MA proteins are in an unfolded, highly flexible state in the MA gland.
Graphical abstract

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Determining hydrogen-bond interactions in spider silk with 1H-13C HETCOR fast MAS solid-state NMR and DFT proton chemical shift calculations. Determining hydrogen-bond interactions in spider silk with 1H-13C HETCOR fast MAS solid-state NMR and DFT proton chemical shift calculations. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Determining hydrogen-bond interactions in spider silk with 1H-13C HETCOR fast MAS solid-state NMR and DFT proton chemical shift calculations. Chem Commun (Camb). 2013 Jul 28;49(59):6680-2 Authors: Holland GP, Mou Q, Yarger JL Abstract Two-dimensional (2D) (1)H-(13)C...	nmrlearner	Journal club	0	01-18-2014 11:31 AM
[NMR paper] Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction. Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction. Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction. Biomacromolecules. 2013 Sep 11; Authors: Sampath S, Jenkins JE, Butler E, Kim J, Henning RW, Holland GP, Yarger JL Abstract This study provides a detailed secondary structural characterization of major ampullate dragline silk from Latrodectus hesperus (black widow) spiders. X-ray diffraction results show...	nmrlearner	Journal club	0	09-13-2013 12:05 PM
[NMR paper] Amino Acid Analysis of spider dragline silk using (1)H NMR. Amino Acid Analysis of spider dragline silk using (1)H NMR. Related Articles Amino Acid Analysis of spider dragline silk using (1)H NMR. Anal Biochem. 2013 May 30; Authors: Shi X, Holland GP, Yarger JL Abstract The amino acid composition of N. clavipes dragline silk fiber is determined by conducting (1)H Nuclear Magnetic Resonance (NMR) spectroscopy experiments on acid hydrolyzed material. N. clavipes dragline silk was found to consist of 43.0±0.6% Gly, 29.3±0.2% Ala, 9.1±0.1% Glx, 4.0±0.1% Leu, 3.3±0.1% Tyr, 3.4 ±0.2% Ser, 2.7±0.1%...	nmrlearner	Journal club	0	06-04-2013 06:31 PM
[NMR paper] Probing site-specific (13)C/ (15)N-isotope enrichment of spider silk with liquid-state NMR spectroscopy. Probing site-specific (13)C/ (15)N-isotope enrichment of spider silk with liquid-state NMR spectroscopy. Probing site-specific (13)C/ (15)N-isotope enrichment of spider silk with liquid-state NMR spectroscopy. Anal Bioanal Chem. 2013 Feb 26; Authors: Shi X, Yarger JL, Holland GP Abstract Solid-state nuclear magnetic resonance (NMR) has been extensively used to elucidate spider silk protein structure and dynamics. In many of these studies, site-specific isotope enrichment is critical for designing particular NMR methods...	nmrlearner	Journal club	0	02-26-2013 06:35 PM
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Comp Biochem Physiol A Mol Integr Physiol. 2011 Jul;159(3):219-24 Authors: Creager MS, Izdebski T, Brooks AE, Lewis RV Abstract Spider silk has been evolutionarily optimized for contextual mechanical performance over the last 400 Ma. Despite precisely balanced mechanical properties,...	nmrlearner	Journal club	0	09-02-2011 05:40 PM
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Chem Commun (Camb). 2010 Sep 28;46(36):6714-6 Authors: Jenkins JE, Creager MS, Butler EB, Lewis RV, Yarger JL, Holland GP Two-dimensional homo- and heteronuclear solid-state MAS NMR experiments on (13)C/(15)N-proline labeled Argiope aurantia dragline silk provide evidence for an elastin-like beta-turn structure for the repetitive Gly-Pro-Gly-X-X motif prevalent in major...	nmrlearner	Journal club	0	12-28-2010 03:31 PM
[NMR paper] NMR characterization of native liquid spider dragline silk from Nephila edulis. NMR characterization of native liquid spider dragline silk from Nephila edulis. Related Articles NMR characterization of native liquid spider dragline silk from Nephila edulis. Biomacromolecules. 2004 May-Jun;5(3):834-9 Authors: Hronska M, van Beek JD, Williamson PT, Vollrath F, Meier BH Solid spider dragline silk is well-known for its mechanical properties. Nonetheless a detailed picture of the spinning process is lacking. Here we report NMR studies on the liquid silk within the wide sac of the major ampullate (m.a.) gland from the spider...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Supercontracted spider dragline silk: a solid-state NMR study of the local structure. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Int J Biol Macromol. 1999 Mar-Apr;24(2-3):173-8 Authors: van Beek JD, KÃ¼mmerlen J, Vollrath F, Meier BH The local structure of supercontracted dragline silk from the spider Nephila madagascariensis was investigated by solid-state nuclear magnetic...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off