NMR paper Existence of isolated H3O+ in the protein interior

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[NMR paper] Existence of isolated H3O+ in the protein interior

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06-15-2017, 03:26 AM

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Existence of isolated H3O+ in the protein interior

Existence of isolated H3O+ in the protein interior

Neutron diffraction analysis studies reported an isolated hydronium ion (H3O+) in the interior of D-xylose isomerase (XI) and phycocyanobilin-ferredoxin oxidoreductase (PcyA). H3O+ forms hydrogen bonds (H-bonds) with two histidine side-chains and a backbone carbonyl group in PcyA, whereas H3O+ forms H-bonds with three acidic residues in XI. Using a quantum mechanical/molecular mechanical (QM/MM) approach, we analyzed stabilization of H3O+ by the protein environment. QM/MM calculations indicated that H3O+ was unstable in the PcyA crystal structure, releasing a proton to an H-bond partner His88, producing H2O and protonated His88. On the other hand, H3O+ was stable in the XI crystal structure. H-bond partners of isolated H3O+ would be practically limited to acidic residues such as aspartic and glutamic acids in the protein environment.

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