Paramagnetic NMR data contain extremely accurate long-range information on metalloprotein structures and, when used in the frame of integrative structural biology approaches, they allow for the retrieval of structural details to a resolution that is not achievable using other techniques. Paramagnetic data thus represent an extremely powerful tool to refine protein models in solution, especially when coupled to X-ray or cryoelectron microscopy data, to monitor the formation of complexes and...
[NMR paper] (19)F NMR as a tool in chemical biology
(19)F NMR as a tool in chemical biology
We previously reviewed the use of ^(19)F NMR in the broad field of chemical biology and present here a summary of the literature from the last decade that has the technique as the central method of analysis. The topics covered include the synthesis of new fluorinated probes and their incorporation into macromolecules, the application of ^(19)F NMR to monitor protein-protein interactions, protein-ligand interactions,...
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02-24-2021 05:50 AM
[NMR paper] 19F NMR as a tool in chemical biology.
19F NMR as a tool in chemical biology.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.png Related Articles 19F NMR as a tool in chemical biology.
Beilstein J Org Chem. 2021;17:293-318
Authors: Gimenez D, Phelan A, Murphy CD, Cobb SL
Abstract
We previously reviewed the use of 19F NMR in the broad field of chemical biology and present here a summary of the literature from the last decade that has the technique as the central method of analysis. The...
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02-15-2021 01:33 PM
[NMR paper] What are the methodological and theoretical prospects for paramagnetic NMR in structural biology? A glimpse into the crystal ball.
What are the methodological and theoretical prospects for paramagnetic NMR in structural biology? A glimpse into the crystal ball.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles What are the methodological and theoretical prospects for paramagnetic NMR in structural biology? A glimpse into the crystal ball.
J Magn Reson. 2019 09;306:173-179
Authors: Ravera E, Parigi G, Luchinat C
Abstract
NMR spectroscopy is very sensitive to the...
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11-04-2020 05:04 PM
[NMR paper] Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
Related Articles Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
J Phys Chem B. 2017 Apr 20;:
Authors: Qin H, Miao Y, Cross TA, Fu R
Abstract
In terms of structural biology, solid-state NMR experiments and strategies have been well established for resonance assignments leading to the...
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04-21-2017 03:35 PM
[NMR paper] A Unique Tool for Cellular Structural Biology: In-cell NMR.
A Unique Tool for Cellular Structural Biology: In-cell NMR.
Related Articles A Unique Tool for Cellular Structural Biology: In-cell NMR.
J Biol Chem. 2015 Dec 16;
Authors: Luchinat E, Banci L
Abstract
Conventional structural and chemical biology approaches are applied to macromolecules extrapolated from their native context. When doing so, important structural and functional features of macromolecules may be lost, which depend on their native network of interactions within the cell. In-cell nuclear magnetic resonance is a branch...
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12-28-2015 12:26 AM
[NMR paper] The IR-(15)N-HSQC-AP experiment: a new tool for NMR spectroscopy of paramagnetic molecules.
The IR-(15)N-HSQC-AP experiment: a new tool for NMR spectroscopy of paramagnetic molecules.
Related Articles The IR-(15)N-HSQC-AP experiment: a new tool for NMR spectroscopy of paramagnetic molecules.
J Biomol NMR. 2014 Jan 11;
Authors: Ciofi-Baffoni S, Gallo A, Muzzioli R, Piccioli M
Abstract
A crucial factor for the understanding of structure-function relationships in metalloproteins is the identification of NMR signals from residues surrounding the metal cofactor. When the latter is paramagnetic, the NMR information in the...
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01-15-2014 05:16 PM
Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology
From The DNP-NMR Blog:
Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology
Gelis, I., et al., Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology. J. Biomol. NMR, 2013. 56(2): p. 85-93.
http://dx.doi.org/10.1007/s10858-013-9721-2
The evolution of paramagnetic NMR as a tool in structural biology
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