NMR paper Ethynylation of Cysteines from Peptides to Proteins in Living Cells

		BioNMR > Educational resources > Journal club
[NMR paper] Ethynylation of Cysteines from Peptides to Proteins in Living Cells

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-01-2020, 12:01 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Ethynylation of Cysteines from Peptides to Proteins in Living Cells

Ethynylation of Cysteines from Peptides to Proteins in Living Cells

Angewandte Chemie International Edition, Accepted Article.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
High-res 3D structure of proteins inside living eukaryotic cells determined - Drug Target Review High-res 3D structure of proteins inside living eukaryotic cells determined - Drug Target Review High-res 3D structure of proteins inside living eukaryotic cells determined Drug Target Review Read here	nmrlearner	Online News	0	02-29-2020 09:52 PM
A Look at Proteins in Living Cells - Science Magazine A Look at Proteins in Living Cells - Science Magazine A Look at Proteins in Living Cells Science MagazineWe spend a lot of time in this sort of work thinking about protein structures. Traditionally that's been the province of X-ray crystallography, later joined by. Read here	nmrlearner	Online News	0	04-03-2019 01:27 PM
[NMR paper] Photoluminescence Lifetime Imaging of Newly Synthesized Proteins in Living Cells with Iridium-alkyne Probe Photoluminescence Lifetime Imaging of Newly Synthesized Proteins in Living Cells with Iridium-alkyne Probe Designing probes for real-time imaging of dynamic processes in living cells is a continual challenge. Herein, a novel near-infrared photoluminescence probe with long lifetime was exploited for photoluminescence lifetime imaging (PLIM) based on an Iridium-alkyne complex. This probe offers benefits of desirable deep-red to NIR emission, long stokes shift, excellent cell penetration, low cytotoxicity and good resistance to photobleaching. To the best of our knowledge this is the first...	nmrlearner	Journal club	0	09-24-2017 06:16 AM
[NMR paper] A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells. A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells. J Biomol NMR. 2016 Sep 8; Authors: Hikone Y, Hirai G, Mishima M, Inomata K, Ikeya T, Arai S, Shirakawa M, Sodeoka M, Ito Y Abstract Structural analyses of...	nmrlearner	Journal club	0	09-22-2016 06:31 AM
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells Abstract Structural analyses of proteins under macromolecular crowding inside human cultured cells by in-cell NMR spectroscopy are crucial not only for explicit understanding of their cellular functions but also for applications in medical and pharmaceutical sciences. In-cell NMR experiments using human cultured cells however suffer from low sensitivity, thus pseudocontact shifts from protein-tagged paramagnetic lanthanoid ions, analysed...	nmrlearner	Journal club	0	09-22-2016 06:26 AM
[NMR paper] Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy. Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy. Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy. Yakugaku Zasshi. 2015;135(3):391-8 Authors: Tochio H, Murayama S, Inomata K, Morimoto D, Ohno A, Shirakawa M Abstract **NMR spectroscopy enables structural analyses of proteins and has been widely used in the structural biology field in recent decades. NMR spectroscopy can be applied to proteins inside living cells, allowing characterization of their structures and dynamics in...	nmrlearner	Journal club	0	03-12-2015 10:33 AM
[NMR paper] High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System. High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System. J Am Chem Soc. 2013 Jan 27; Authors: Hamatsu J, O'Donovan D, Tanaka T, Shirai T, Hourai Y, Mikawa T, Ikeya T, Mishima M, Boucher W, Smith BO, Laue ED, Shirakawa M, Ito Y ...	nmrlearner	Journal club	0	02-03-2013 10:19 AM
High-Resolution HeteronuclearMultidimensional NMRof Proteins in Living Insect Cells Using a Baculovirus Protein ExpressionSystem High-Resolution HeteronuclearMultidimensional NMRof Proteins in Living Insect Cells Using a Baculovirus Protein ExpressionSystem Jumpei Hamatsu, Daniel O’Donovan, Takashi Tanaka, Takahiro Shirai, Yuichiro Hourai, Tsutomu Mikawa, Teppei Ikeya, Masaki Mishima, Wayne Boucher, Brian O. Smith, Ernest D. Laue, Masahiro Shirakawa and Yutaka Ito http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja310928u/aop/images/medium/ja-2012-10928u_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja310928u...	nmrlearner	Journal club	0	01-27-2013 11:41 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off