ABSTRACT: BACKGROUND: The lung would be the first organ targeted in case of the use of Variola virus (the causative agent of smallpox) as a bioweapon. Pulmonary surfactant composed of lipids (90%) and proteins (10%) is considered the major physiological barrier against airborne pathogens. The principle phospholipid components of lung surfactant were examined in an in vitro model to characterize their interactions with VACV, a surrogate for variola virus. One of them, Dipalmitoyl phosphatidylglycerol (DPPG), was recently shown to inhibit VACV cell infection. RESULTS: The interactions of poxvirus particles from the Western Reserve strain (VACV-WR) and the Lister strain (VACV-List) with model membranes for pulmonary surfactant phospholipids, in particular DPPG, were studied by Electron Spin Resonance (ESR) and proton Nuclear Magnetic Resonance (1H-NMR). ESR experiments showed that DPPG exhibits specific interactions with both viruses, while NMR experiments allowed us to deduce its stoichiometry and to propose a model for the mechanism of interaction at the molecular level. CONCLUSIONS: These results confirm the ability of DPPG to strongly bind to VACV and suggest that similar interactions occur with variola virus. Similar studies of the interactions between lipids and other airborne pathogens are warranted.
PMID: 21194478 [PubMed - as supplied by publisher]
Glycerol and Glycerol Carbonate as ultraviscous solvents for mixture analysis by NMR
Glycerol and Glycerol Carbonate as ultraviscous solvents for mixture analysis by NMR
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 1 July 2011</br>
Pedro, Lameiras , Leslie, Boudesocque , Zéphirin, Mouloungui , Jean-Hugues, Renault , Jean-Michel, Wieruszeski , ...</br>
NMR of weakly polar analytes in an apolar ultraviscous solvent has recently been proposed for mixture analysis as a pertinent alternative to the DOSY experiment. The present article reports the first use of glycerol and glycerol carbonate as polar...
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ESR and NMR studies provide evidence that phosphatidyl glycerol specifically interacts with poxvirus membranes.
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