The Escherichia coli BolA ProteinIbaG Forms a Histidine-Ligated [2Fe-2S]-Bridged Complex with Grx4

		BioNMR > Educational resources > Journal club
The Escherichia coli BolA ProteinIbaG Forms a Histidine-Ligated [2Fe-2S]-Bridged Complex with Grx4

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-02-2016, 07:45 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

The Escherichia coli BolA ProteinIbaG Forms a Histidine-Ligated [2Fe-2S]-Bridged Complex with Grx4

The Escherichia coli BolA ProteinIbaG Forms a Histidine-Ligated [2Fe-2S]-Bridged Complex with Grx4

Biochemistry
DOI: 10.1021/acs.biochem.6b00812

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Strategies for Protein NMR in Escherichia coli. Strategies for Protein NMR in Escherichia coli. Related Articles Strategies for Protein NMR in Escherichia coli. Biochemistry. 2014 Mar 5; Authors: Xu G, Ye Y, Liu X, Cao S, Wu Q, Cheng K, Liu M, Pielak GJ, Li C Abstract In-cell NMR spectroscopy provides insight into protein conformation, dynamics and function at atomic resolution in living cells. Systematic evaluation of isotopic labeling strategies is necessary to observe the target protein in the sea of other molecules in the cell. Here, we investigate the detectability, sensitivity and...	nmrlearner	Journal club	0	03-08-2014 06:52 AM
[NMR paper] Solution NMR structure of the 48-kDa IIAMannose-HPr complex of the Escherichia coli m Solution NMR structure of the 48-kDa IIAMannose-HPr complex of the Escherichia coli mannose phosphotransferase system. Related Articles Solution NMR structure of the 48-kDa IIAMannose-HPr complex of the Escherichia coli mannose phosphotransferase system. J Biol Chem. 2005 May 27;280(21):20775-84 Authors: Williams DC, Cai M, Suh JY, Peterkofsky A, Clore GM The solution structure of the 48-kDa IIA(Man)-HPr complex of the mannose branch of the Escherichia coli phosphotransferase system has been solved by NMR using conjoined rigid body/torsion...	nmrlearner	Journal club	0	11-24-2010 11:14 PM
[NMR paper] NMR structure of ribonuclease HI from Escherichia coli. NMR structure of ribonuclease HI from Escherichia coli. Related Articles NMR structure of ribonuclease HI from Escherichia coli. Biol Pharm Bull. 2000 Oct;23(10):1147-52 Authors: Fujiwara M, Kato T, Yamazaki T, Yamasaki K, Nagayam K The solution structure of ribonuclease HI (RNase HI) from Escherichia coli (E. coli), a protein of 155 residues, was determined. Three-dimensional nuclear Overhauser enhancement spectroscopy (NOESY) was used to obtain 1,424 distance constraints between individually assigned polypeptide chain hydrogen atoms....	nmrlearner	Journal club	0	11-19-2010 08:29 PM
[NMR paper] NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ. NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ. Related Articles NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ. Nature. 1998 Nov 5;396(6706):88-92 Authors: Tanaka T, Saha SK, Tomomori C, Ishima R, Liu D, Tong KI, Park H, Dutta R, Qin L, Swindells MB, Yamazaki T, Ono AM, Kainosho M, Inouye M, Ikura M Bacteria live in capricious environments, in which they must continuously sense external conditions in order to adjust their shape, motility and physiology. The histidine-aspartate...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] The Aspergillus nidulans transcription factor AlcR forms a stable complex with its ha The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study. FEBS Lett. 1997 May 19;408(2):235-40 Authors: Cerdan R, Collin D, Lenouvel F, Felenbok B, Guittet E The Aspergillus nidulans transcription factor AlcR is shown by NMR and gel retardation assay to form a...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] The Aspergillus nidulans transcription factor AlcR forms a stable complex with its ha The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study. FEBS Lett. 1997 May 19;408(2):235-40 Authors: Cerdan R, Collin D, Lenouvel F, Felenbok B, Guittet E The Aspergillus nidulans transcription factor AlcR is shown by NMR and gel retardation assay to form a...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] NMR studies of the Escherichia coli Trp repressor.trpRs operator complex. NMR studies of the Escherichia coli Trp repressor.trpRs operator complex. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles NMR studies of the Escherichia coli Trp repressor.trpRs operator complex. Eur J Biochem. 1996 Dec 15;242(3):567-75 Authors: Evans PD, Jaseja M, Jeeves M, Hyde EI To understand the specificity of the Escherichia coli Trp repressor for its operators, we have begun to study complexes of the protein with...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] NMR structure of Escherichia coli glutaredoxin 3-glutathione mixed disulfide complex: NMR structure of Escherichia coli glutaredoxin 3-glutathione mixed disulfide complex: implications for the enzymatic mechanism. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR structure of Escherichia coli glutaredoxin 3-glutathione mixed disulfide complex: implications for the enzymatic mechanism. J Mol Biol. 1999 Feb 19;286(2):541-52 Authors: Nordstrand K, slund F, Holmgren A, Otting G, Berndt KD Glutaredoxins (Grxs) catalyze reversible oxidation/reduction of...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off