NMR paper Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.

		BioNMR > Educational resources > Journal club
[NMR paper] Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-12-2017, 12:32 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.

Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.

Related Articles Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.

Protein Cell. 2017 Mar 10;:

Authors: Li D, Zhang Y, He Y, Zhang C, Wang J, Xiong Y, Zhang L, Liu Y, Shi P, Tian C

PMID: 28284007 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Bacterial production of site specific 13 C labeled phenylalanine and methodology for high level incorporation into bacterially expressed recombinant proteins Bacterial production of site specific 13 C labeled phenylalanine and methodology for high level incorporation into bacterially expressed recombinant proteins Abstract Nuclear magnetic resonance spectroscopy studies of ever larger systems have benefited from many different forms of isotope labeling, in particular, site specific isotopic labeling. Site specific 13C labeling of methyl groups has become an established means of probing systems not amenable to traditional methodology. However useful, methyl reporter sites can be limited in number and/or...	nmrlearner	Journal club	0	12-27-2016 11:04 PM
NMR-BasedDetermination of the 3D Structure of theLigand–Protein Interaction Site without Protein Resonance Assignment NMR-BasedDetermination of the 3D Structure of theLigand–Protein Interaction Site without Protein Resonance Assignment Julien Orts, Marielle Aulikki Wa?lti, May Marsh, Laura Vera, Alvar D. Gossert, Peter Gu?ntert and Roland Riek http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b12391/20160323/images/medium/ja-2015-12391s_0002.gif Journal of the American Chemical Society DOI: 10.1021/jacs.5b12391 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/np60JwjsRiM	nmrlearner	Journal club	0	03-24-2016 04:18 AM
[NMR paper] NMR-based determination of the 3D structure of the ligand-protein interaction site without protein resonance assignment. NMR-based determination of the 3D structure of the ligand-protein interaction site without protein resonance assignment. NMR-based determination of the 3D structure of the ligand-protein interaction site without protein resonance assignment. J Am Chem Soc. 2016 Mar 4; Authors: Orts J, Wälti MA, Marsh M, Vera L, Gossert AD, Güntert P, Riek R Abstract Molecular replacement in X-ray crystallography is the prime method for establishing structure-activity relationships of pharmaceutically relevant molecules. Such an approach is not...	nmrlearner	Journal club	0	03-05-2016 11:21 AM
Persistent Activation of cGMP-Dependent Protein Kinaseby a Nitrated Cyclic Nucleotide via Site Specific Protein S-Guanylation Persistent Activation of cGMP-Dependent Protein Kinaseby a Nitrated Cyclic Nucleotide via Site Specific Protein S-Guanylation http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.5b00774/20160129/images/medium/bi-2015-00774n_0003.gif Biochemistry DOI: 10.1021/acs.biochem.5b00774 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/KLjU9u3Nnwk More...	nmrlearner	Journal club	0	01-30-2016 07:56 AM
[NMR paper] A chemical approach for site-specific identification of NMR signals from protein side-chain NH3 (+) groups forming intermolecular ion pairs in protein-nucleic acid complexes. A chemical approach for site-specific identification of NMR signals from protein side-chain NH3 (+) groups forming intermolecular ion pairs in protein-nucleic acid complexes. Related Articles A chemical approach for site-specific identification of NMR signals from protein side-chain NH3 (+) groups forming intermolecular ion pairs in protein-nucleic acid complexes. J Biomol NMR. 2015 Feb 19; Authors: Anderson KM, Nguyen D, Esadze A, Zandrashvili L, Gorenstein DG, Iwahara J Abstract Protein-nucleic acid interactions involve...	nmrlearner	Journal club	0	02-19-2015 07:03 PM
[NMR paper] A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids. A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids. Related Articles A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids. Isr J Chem. 2014 Feb 1;54(1-2):171-183 Authors: Hou G, Gupta R, Polenova T, Vega AJ Abstract Proton chemical shifts are a rich probe of structure and hydrogen bonding environments in organic and biological molecules. Until recently,...	nmrlearner	Journal club	0	12-09-2014 01:13 PM
[NMR paper] Site-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy. Site-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Site-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy. Angew Chem Int Ed Engl. 2012 Dec 21;51(52):13140-3 Authors: Madine J, Pandya MJ, Hicks MR, Rodger A, Yates EA, Radford SE, Middleton DA Abstract At the...	nmrlearner	Journal club	0	06-12-2013 11:42 AM
[NMR paper] Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates. Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates. Related Articles Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates. J Am Chem Soc. 2013 Feb 11; Authors: Maltsev AS, Chen J, Levine RL, Bax A Abstract ?-Synuclein (aS) is an intrinsically disordered protein that is water soluble but also can bind negatively charged lipid membranes while adopting an ?-helical conformation. Membrane affinity is increased by...	nmrlearner	Journal club	0	02-13-2013 12:47 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off