Mobile proton containing solutes can be detected by MRI via Chemical Exchange Saturation Transfer(CEST)method. The CEST sensitivity is dramatically enhanced by using, as exchanging protons, the water molecules confined inside liposomes, properly shifted by a paramagnetic Shift Reagent. The chemical shift of the intraliposomal water resonance(?IL) is affected by the overall shape of the supramolecular system. Herein, it is shown that ?IL of a spherical LipoCEST acts as a sensitive reporter of the distribution of Streptavidin proteins anchored at the Liposomes'surface by biotinylated phospholipids.These observations prompted the design of a MMP-2 responsive LipoCEST agent as the Streptavidin moieties can be released from the Liposomes' surfaces when a properly tailored enzyme-cleavable peptide is inserted on the phospholipids before the terminal biotin residues. ?IL reports on the overall changes in the supramolecular architecture associated to the cleavage carried out by MMP-2.
Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins
From The DNP-NMR Blog:
Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins
Vuichoud, B., et al., Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins. Chemistry, 2016: p. n/a-n/a.
https://www.ncbi.nlm.nih.gov/pubmed/27546550
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09-28-2016 06:22 PM
[NMR paper] Intra- and inter-molecular interactions of human galectin-3: assessment by full-assignment-based NMR.
Intra- and inter-molecular interactions of human galectin-3: assessment by full-assignment-based NMR.
Related Articles Intra- and inter-molecular interactions of human galectin-3: assessment by full-assignment-based NMR.
Glycobiology. 2016 Feb 23;
Authors: Ippel H, Miller MC, Vértesy S, Zhang Y, Cañada FJ, Suylen D, Umemoto K, Romanò C, Hackeng T, Tai G, Leffler H, Kopitz J, André S, Kübler D, Jiménez-Barbero J, Oscarson S, Gabius HJ, Mayo KH
Abstract
Galectin-3 is an adhesion/growth-regulatory protein with a modular design...
Sub-second proton imaging of 13C hyperpolarized contrast agents in water
From The DNP-NMR Blog:
Sub-second proton imaging of 13C hyperpolarized contrast agents in water
Truong, M.L., et al., Sub-second proton imaging of 13C hyperpolarized contrast agents in water. Contrast Media Mol Imaging, 2014. 9(5): p. 333-41.
http://www.ncbi.nlm.nih.gov/pubmed/24753438
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03-11-2015 09:59 PM
[NMR paper] Relation of enzyme activity to local/global stability of murine adenosine deaminase:
Relation of enzyme activity to local/global stability of murine adenosine deaminase: 19F NMR studies.
Related Articles Relation of enzyme activity to local/global stability of murine adenosine deaminase: 19F NMR studies.
J Mol Biol. 2005 Jan 21;345(3):599-610
Authors: Shu Q, Frieden C
Adenosine deaminase (ADA, EC 3.5.4.4) is a ubiquitous (beta/alpha)8-barrel enzyme crucial for purine metabolism and normal immune competence. In this study, it was observed that loss of enzyme activity of murine ADA (mADA) precedes the global secondary and...
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11-24-2010 11:14 PM
[NMR paper] Measuring enzyme hydration in nonpolar organic solvents using NMR.
Measuring enzyme hydration in nonpolar organic solvents using NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Measuring enzyme hydration in nonpolar organic solvents using NMR.
Biotechnol Bioeng. 1995 Jun 5;46(5):452-8
Authors: Parker MC, Moore BD, Blacker AJ
A very sensitive NMR method has been developed for measuring deuterated water bound to proteins suspended in nonpolar solvents. This has been used to determine the amount of...
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08-22-2010 03:41 AM
A simple method for measuring signs of 1HN chemical shift differences between ground
Abstract NMR relaxation dispersion spectroscopy is a powerful method for studying protein conformational dynamics whereby visible, ground and invisible, excited conformers interconvert on the millisecond time-scale. In addition to providing kinetics and thermodynamics parameters of the exchange process, the CPMG dispersion experiment also allows extraction of the absolute values of the chemical shift differences between interconverting states,
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ê , opening the way for structure determination of excited state conformers. Central to the goal of structural...
Enzyme responsive LipoCEST agents: assessment of MMP-2 activity by measuring the intra-liposomal water 1H-NMR resonance shift
Linear Mode
