Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated Ï? pulses

		BioNMR > Educational resources > Journal club
Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated Ï? pulses

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-21-2017, 10:10 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated Ï? pulses

Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated Ï? pulses

Abstract

In multidimensional solution NMR experiments, Ï? pulses are used extensively for inversion and refocusing operations on 1H, 13C and 15N nuclei. Pulse miscalibration, off-resonance effects, and J-coupling evolution during Ï? pulse execution result in severe signal losses that are exacerbated at high magnetic fields. Here, we report the implementation of a triply-compensated Ï? pulse (G5) optimized for both inversion and refocusing in widely used 2- and 3-dimensional experiments. By replacing most of the hard Ï? pulses, adiabatic or composite pulses on the 1H, 13C and 15N channels with G5 pulses, we obtained signal enhancements ranging from 80 to 240%. We anticipate that triply-compensated pulses will be crucial for improving the performance of multidimensional and multinuclear pulse sequences at ultra-high fields.

Source: Journal of Biomolecular NMR

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Genetic Algorithm Optimized Triply Compensated Pulses in NMR Spectroscopy Genetic Algorithm Optimized Triply Compensated Pulses in NMR Spectroscopy Publication date: Available online 28 September 2015 Source:Journal of Magnetic Resonance</br> Author(s): V.S. Manu, Gianluigi Veglia</br> Sensitivity and resolution in NMR experiments are affected by magnetic field inhomogeneities (of both external and RF), errors in pulse calibration, and offset effects due to finite length of RF pulses. To remedy these problems, built-in compensation mechanisms for these experimental imperfections are often necessary. Here, we propose a new family of...	nmrlearner	Journal club	0	09-29-2015 07:59 AM
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments From The DNP-NMR Blog: Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments Takahashi, H., et al., Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments. J Magn Reson, 2013. 239C(0): p. 91-99. http://www.ncbi.nlm.nih.gov/pubmed/24480716	nmrlearner	News from NMR blogs	0	02-10-2014 08:46 PM
[NMR paper] Homonuclear decoupling for enhancing resolution and sensitivity in NOE and RDC measurements of peptides and proteins Homonuclear decoupling for enhancing resolution and sensitivity in NOE and RDC measurements of peptides and proteins Publication date: Available online 22 November 2013 Source:Journal of Magnetic Resonance</br> Author(s): Jinfa Ying , Julien Roche , Ad Bax</br> Application of band-selective homonuclear (BASH) 1H decoupling pulses during acquisition of the 1H free induction decay is shown to be an efficient procedure for removal of scalar and residual dipolar couplings between amide and aliphatic protons. BASH decoupling can be applied in both dimensions of a...	nmrlearner	Journal club	0	11-23-2013 04:05 AM
[NMR paper] The Fantastic Four: A plug 'n' play set of optimal control pulses for enhancing NMR spectroscopy. From Mendeley Biomolecular NMR group: The Fantastic Four: A plug 'n' play set of optimal control pulses for enhancing NMR spectroscopy. Journal of magnetic resonance (San Diego, Calif. : 1997) (2013). Pages: 16-31. Manoj Nimbalkar, Burkhard Luy, Thomas E Skinner, Jorge L Neves, Naum I Gershenzon, Kyryl Kobzar, Wolfgang Bermel, Steffen J Glaser et al. We present highly robust, optimal control-based shaped pulses designed to replace all 90° and 180° hard pulses in a given pulse sequence for improved performance. Special attention was devoted to ensuring that the pulses can be simply...	nmrlearner	Journal club	0	10-17-2013 12:49 PM
[NMR paper] The Fantastic Four: A plug 'n' play set of optimal control pulses for enhancing NMR spectroscopy. From Mendeley Biomolecular NMR group: The Fantastic Four: A plug 'n' play set of optimal control pulses for enhancing NMR spectroscopy. Journal of magnetic resonance (San Diego, Calif. : 1997) (2013). Pages: 16-31. Manoj Nimbalkar, Burkhard Luy, Thomas E Skinner, Jorge L Neves, Naum I Gershenzon, Kyryl Kobzar, Wolfgang Bermel, Steffen J Glaser et al. We present highly robust, optimal control-based shaped pulses designed to replace all 90° and 180° hard pulses in a given pulse sequence for improved performance. Special attention was devoted to ensuring that the pulses can be simply...	nmrlearner	Journal club	0	04-11-2013 03:08 PM
Practical aspects of high-sensitivity multidimensional 13C MAS NMR spectroscopy of perdeuterated proteins Practical aspects of high-sensitivity multidimensional 13C MAS NMR spectroscopy of perdeuterated proteins April 2012 Publication year: 2012 Source:Journal of Magnetic Resonance, Volume 217</br> </br> The double nucleus enhanced recoupling (DONER) experiment employs simultaneous irradiation of protons and deuterons to promote spin diffusion processes in a perdeuterated protein. This results in 4–5times higher sensitivity in 2D 13C–13C correlation experiments as compared to PDSD . Here, a quantitative comparison of PDSD, 1H-DARR, 2H-DARR, and 1H+ 2H DONER has been...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Practical Aspects of High-Sensitivity Multidimensional 13C MAS NMR Spectroscopy of Perdeuterated Proteins Practical Aspects of High-Sensitivity Multidimensional 13C MAS NMR Spectroscopy of Perdeuterated Proteins Publication year: 2012 Source:Journal of Magnetic Resonance</br> Ümit Akbey, Barth-Jan van Rossum, Hartmut Oschkinat</br> The double nucleus enhanced recoupling (DONER) experiment employs simultaneous irradiation of protons and deuterons to promote spin diffusion processes in a perdeuterated protein. This results in 4-5 times higher sensitivity in 2D 13C-13C correlation experiments as compared to PDSD. Here, a quantitative comparison of PDSD, 1H-DARR, 2H-DARR, and...	nmrlearner	Journal club	0	03-09-2012 09:16 AM
[NMR tweet] Got my PhD viva on Thursday. "Enhancing the sensitivity of nuclear magnetic resonance with dynamic nuclear polarisation." - Cramming! Got my PhD viva on Thursday. "Enhancing the sensitivity of nuclear magnetic resonance with dynamic nuclear polarisation." - Cramming! Published by msinternet (MS Internet) on 2011-01-17T19:24:07Z Source: Twitter	nmrlearner	Twitter NMR	0	01-17-2011 07:30 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off