[NMR paper] Elucidation of the CCR1- and CCR5-binding modes of MIP-1? by application of an NMR spectra reconstruction method to the transferred cross-saturation experiments.
Elucidation of the CCR1- and CCR5-binding modes of MIP-1? by application of an NMR spectra reconstruction method to the transferred cross-saturation experiments.
Related ArticlesElucidation of the CCR1- and CCR5-binding modes of MIP-1? by application of an NMR spectra reconstruction method to the transferred cross-saturation experiments.
J Biomol NMR. 2015 Oct 15;
Authors: Yoshiura C, Ueda T, Kofuku Y, Matsumoto M, Okude J, Kondo K, Shiraishi Y, Shimada I
Abstract
C-C chemokine receptor 1 (CCR1) and CCR5 are involved in various inflammation and immune responses, and regulate the progression of the autoimmune diseases differently. However, the number of residues identified at the binding interface was not sufficient to clarify the differences in the CCR1- and CCR5-binding modes to MIP-1?, because the NMR measurement time for CCR1 and CCR5 samples was limited to 24*h, due to their low stability. Here we applied a recently developed NMR spectra reconstruction method, Conservation of experimental data in ANAlysis of FOuRier, to the amide-directed transferred cross-saturation experiments of chemokine receptors, CCR1 and CCR5, embedded in lipid bilayers of the reconstituted high density lipoprotein, and MIP-1?. Our experiments revealed that the residues on the N-loop and ?-sheets of MIP-1? are close to both CCR1 and CCR5, and those in the C-terminal helix region are close to CCR5. These results suggest that the genetic influence of the single nucleotide polymorphisms of MIP-1? that accompany substitution of residues in the C-terminal helix region, E57 and V63, would provide clues toward elucidating how the CCR5-MIP-1? interaction affects the progress of autoimmune diseases.
PMID: 26472202 [PubMed - as supplied by publisher]
Elucidation of the CCR1- and CCR5-binding modes of MIP-1α by application of an NMR spectra reconstruction method to the transferred cross-saturation experiments
Elucidation of the CCR1- and CCR5-binding modes of MIP-1α by application of an NMR spectra reconstruction method to the transferred cross-saturation experiments
Abstract
Câ??C chemokine receptor 1 (CCR1) and CCR5 are involved in various inflammation and immune responses, and regulate the progression of the autoimmune diseases differently. However, the number of residues identified at the binding interface was not sufficient to clarify the differences in the CCR1- and CCR5-binding modes to MIP-1α, because the NMR measurement time for CCR1 and CCR5...
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10-15-2015 12:19 PM
Langerin–HeparinInteraction: Two Binding Sitesfor Small and Large Ligands As Revealed by a Combination of NMR Spectroscopyand Cross-Linking Mapping Experiments
Langerin–HeparinInteraction: Two Binding Sitesfor Small and Large Ligands As Revealed by a Combination of NMR Spectroscopyand Cross-Linking Mapping Experiments
Juan C. Mun?oz-Garci?a, Eric Chabrol, Romain R. Vive?s, Aline Thomas, Jose? L. de Paz, Javier Rojo, Anne Imberty, Franck Fieschi, Pedro M. Nieto and Jesu?s Angulo
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja511529x/20150319/images/medium/ja-2014-11529x_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/ja511529x...
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03-23-2015 08:22 PM
[NMR paper] Langerin-Heparin Interaction: Two Binding Sites for Small and Large Ligands as revealed by a combination of NMR Spectroscopy and Cross-Linking Mapping Experiments.
Langerin-Heparin Interaction: Two Binding Sites for Small and Large Ligands as revealed by a combination of NMR Spectroscopy and Cross-Linking Mapping Experiments.
Related Articles Langerin-Heparin Interaction: Two Binding Sites for Small and Large Ligands as revealed by a combination of NMR Spectroscopy and Cross-Linking Mapping Experiments.
J Am Chem Soc. 2015 Mar 6;
Authors: Muñoz-García JC, Chabrol E, Vives RR, Thomas A, de Paz JL, Rojo J, Imberty A, Fieschi F, Nieto PM, Angulo J
Abstract
Langerin is a C-type lectin present...
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03-10-2015 07:22 PM
Development of a method for reconstruction of crowded NMR spectra from undersampled time-domain data
Development of a method for reconstruction of crowded NMR spectra from undersampled time-domain data
Abstract
NMR is a unique methodology for obtaining information about the conformational dynamics of proteins in heterogeneous biomolecular systems. In various NMR methods, such as transferred cross-saturation, relaxation dispersion, and paramagnetic relaxation enhancement experiments, fast determination of the signal intensity ratios in the NMR spectra with high accuracy is required for analyses of targets with low yields and stabilities. However,...
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02-14-2015 03:52 PM
[NMR paper] Development of a method for reconstruction of crowded NMR spectra from undersampled time-domain data.
Development of a method for reconstruction of crowded NMR spectra from undersampled time-domain data.
Related Articles Development of a method for reconstruction of crowded NMR spectra from undersampled time-domain data.
J Biomol NMR. 2015 Feb 13;
Authors: Ueda T, Yoshiura C, Matsumoto M, Kofuku Y, Okude J, Kondo K, Shiraishi Y, Takeuchi K, Shimada I
Abstract
NMR is a unique methodology for obtaining information about the conformational dynamics of proteins in heterogeneous biomolecular systems. In various NMR methods, such as...
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02-14-2015 03:52 PM
[NMR paper] Comparing Binding Modes of Analogous Fragments Using NMR in Fragment-Based Drug Design: Application to PRDX5.
Comparing Binding Modes of Analogous Fragments Using NMR in Fragment-Based Drug Design: Application to PRDX5.
Comparing Binding Modes of Analogous Fragments Using NMR in Fragment-Based Drug Design: Application to PRDX5.
PLoS One. 2014;9(7):e102300
Authors: Aguirre C, Brink TT, Guichou JF, Cala O, Krimm I
Abstract
Fragment-based drug design is one of the most promising approaches for discovering novel and potent inhibitors against therapeutic targets. The first step of the process consists of identifying fragments that bind...
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07-16-2014 10:46 AM
Compressed sensing reconstruction of undersampled 3D NOESY spectra: application to large membrane proteins
Compressed sensing reconstruction of undersampled 3D NOESY spectra: application to large membrane proteins
Abstract Central to structural studies of biomolecules are multidimensional experiments. These are lengthy to record due to the requirement to sample the full Nyquist grid. Time savings can be achieved through undersampling the indirectly-detected dimensions combined with non-Fourier Transform (FT) processing, provided the experimental signal-to-noise ratio is sufficient. Alternatively, resolution and signal-to-noise can be improved within a given experiment time. However, non-FT...
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07-30-2012 07:42 AM
[NMR paper] Generalized reconstruction of n-D NMR spectra from multiple projections: application
Generalized reconstruction of n-D NMR spectra from multiple projections: application to the 5-D HACACONH spectrum of protein G B1 domain.
Related Articles Generalized reconstruction of n-D NMR spectra from multiple projections: application to the 5-D HACACONH spectrum of protein G B1 domain.
J Am Chem Soc. 2004 Feb 4;126(4):1000-1
Authors: Coggins BE, Venters RA, Zhou P
Reconstructing multidimensional NMR spectra from 2-D projections significantly reduces the time needed for data collection over conventional methodology. Here, we provide a...
Elucidation of the CCR1- and CCR5-binding modes of MIP-1? by application of an NMR spectra reconstruction method to the transferred cross-saturation experiments.
Linear Mode
