Related ArticlesElucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR.
Chem Commun (Camb). 2014 May 14;50(37):4856-9
Authors: Shi X, Yarger JL, Holland GP
Abstract
(2)H-(13)C HETCOR MAS NMR is performed on (2)H/(13)C/(15)N-Pro enriched A. aurantia dragline silk. Proline dynamics are extracted from (2)H NMR line shapes and T1 in a site-specific manner to elucidate the backbone and side chain molecular dynamics for the MaSp2 GPGXX ?-turn regions for spider dragline silk in the dry and wet, supercontracted states.
[NMR paper] Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR.
Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR.
Biomacromolecules. 2015 Jan 25;
Authors: Shi X, Holland GP, Yarger JL
Abstract
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01-28-2015 03:22 AM
Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy
Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution-state NMR Spectroscopy
Publication date: Available online 13 June 2014
Source:Polymer</br>
Author(s): Dian Xu , Jeffery L. Yarger , Gregory P. Holland</br>
Spider dragline silk is an outstanding biopolymer with a strength that exceeds steel by weight and a toughness greater than high-performance fibers like Kevlar. For this reason, understanding how a spider converts the gel-like, aqueous protein spinning dope within the major ampullate (MA) gland into a super...
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06-14-2014 12:53 AM
[NMR paper] Determining hydrogen-bond interactions in spider silk with 1H-13C HETCOR fast MAS solid-state NMR and DFT proton chemical shift calculations.
Determining hydrogen-bond interactions in spider silk with 1H-13C HETCOR fast MAS solid-state NMR and DFT proton chemical shift calculations.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Determining hydrogen-bond interactions in spider silk with 1H-13C HETCOR fast MAS solid-state NMR and DFT proton chemical shift calculations.
Chem Commun (Camb). 2013 Jul 28;49(59):6680-2
Authors: Holland GP, Mou Q, Yarger JL
Abstract
Two-dimensional (2D) (1)H-(13)C...
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01-18-2014 11:31 AM
[NMR paper] Amino Acid Analysis of spider dragline silk using (1)H NMR.
Amino Acid Analysis of spider dragline silk using (1)H NMR.
Related Articles Amino Acid Analysis of spider dragline silk using (1)H NMR.
Anal Biochem. 2013 May 30;
Authors: Shi X, Holland GP, Yarger JL
Abstract
The amino acid composition of N. clavipes dragline silk fiber is determined by conducting (1)H Nuclear Magnetic Resonance (NMR) spectroscopy experiments on acid hydrolyzed material. N. clavipes dragline silk was found to consist of 43.0±0.6% Gly, 29.3±0.2% Ala, 9.1±0.1% Glx, 4.0±0.1% Leu, 3.3±0.1% Tyr, 3.4 ±0.2% Ser, 2.7±0.1%...
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06-04-2013 06:31 PM
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Comp Biochem Physiol A Mol Integr Physiol. 2011 Jul;159(3):219-24
Authors: Creager MS, Izdebski T, Brooks AE, Lewis RV
Abstract
Spider silk has been evolutionarily optimized for contextual mechanical performance over the last 400 Ma. Despite precisely balanced mechanical properties,...
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09-02-2011 05:40 PM
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk.
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk.
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk.
Chem Commun (Camb). 2010 Sep 28;46(36):6714-6
Authors: Jenkins JE, Creager MS, Butler EB, Lewis RV, Yarger JL, Holland GP
Two-dimensional homo- and heteronuclear solid-state MAS NMR experiments on (13)C/(15)N-proline labeled Argiope aurantia dragline silk provide evidence for an elastin-like beta-turn structure for the repetitive Gly-Pro-Gly-X-X motif prevalent in major...
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12-28-2010 03:31 PM
[NMR paper] NMR characterization of native liquid spider dragline silk from Nephila edulis.
NMR characterization of native liquid spider dragline silk from Nephila edulis.
Related Articles NMR characterization of native liquid spider dragline silk from Nephila edulis.
Biomacromolecules. 2004 May-Jun;5(3):834-9
Authors: Hronska M, van Beek JD, Williamson PT, Vollrath F, Meier BH
Solid spider dragline silk is well-known for its mechanical properties. Nonetheless a detailed picture of the spinning process is lacking. Here we report NMR studies on the liquid silk within the wide sac of the major ampullate (m.a.) gland from the spider...
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11-24-2010 09:51 PM
[NMR paper] Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
Int J Biol Macromol. 1999 Mar-Apr;24(2-3):173-8
Authors: van Beek JD, Kümmerlen J, Vollrath F, Meier BH
The local structure of supercontracted dragline silk from the spider Nephila madagascariensis was investigated by solid-state nuclear magnetic...
Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR.
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