In NMR spectroscopy, residual dipolar couplings (RDCs) have emerged as one of the most exquisite probes of biological structure and dynamics. The measurement of RDCs relies on the partial alignment of the molecule of interest, for example by using a liquid crystal as a solvent. Here, we establish bacterial type 1 pili as an alternative liquid-crystalline alignment medium for the measurement of RDCs. To achieve alignment at pilus concentrations that allow for efficient NMR sample preparation, we...
[NMR paper] Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems.
Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems.
Prog Nucl Magn Reson Spectrosc. 2020 Feb;116:56-84
Authors: Schütz S, Sprangers R
Abstract
A major goal in structural biology is to unravel how molecular machines function in detail. To that end,...
nmrlearner
Journal club
0
03-07-2020 06:20 PM
Characterization of H/D exchange in type 1 pili by proton-detected solid-state NMR and molecular dynamics simulations
Characterization of H/D exchange in type 1 pili by proton-detected solid-state NMR and molecular dynamics simulations
Abstract
Uropathogenic Escherichia coli invades and colonizes hosts by attaching to cells using adhesive pili on the bacterial surface. Although many biophysical techniques have been used to study the structure and mechanical properties of pili, many important details are still unknown. Here we use proton-detected solid-state NMR experiments to investigate solvent accessibility and structural dynamics. Deuterium back-exchange at labile...
nmrlearner
Journal club
0
04-26-2019 03:47 PM
[NMR paper] Protons as Versatile Reporters in Solid-State NMR Spectroscopy.
Protons as Versatile Reporters in Solid-State NMR Spectroscopy.
Related Articles Protons as Versatile Reporters in Solid-State NMR Spectroscopy.
Acc Chem Res. 2018 May 15;:
Authors: Vasa SK, Rovó P, Linser R
Abstract
Solid-state nuclear magnetic resonance (ssNMR) is a spectroscopic technique that is used for characterization of molecular properties in the solid phase at atomic resolution. In particular, using the approach of magic-angle spinning (MAS), ssNMR has seen widespread applications for topics ranging from material...
nmrlearner
Journal club
0
05-16-2018 03:42 PM
[NMR paper] Self-assembled Oligopeptide as a Versatile NMR Alignment Medium for the Measurements of Residual Dipolar Couplings in Methanol
Self-assembled Oligopeptide as a Versatile NMR Alignment Medium for the Measurements of Residual Dipolar Couplings in Methanol
Residual dipolar coupling (RDC) constitutes a powerful structural parameter for the determination of constitution, conformation and configuration of organic molecules. Here, we report the first liquid crystalline based orientating medium that is compatible with MeOH, thus enabling RDC acquisitions of a wide range of intermediate to polar organic molecules. The liquid crystals were produced by the self-assembled oligopeptide nanotubes (AAKLVFF), which are stable...
nmrlearner
Journal club
0
08-23-2017 05:25 PM
Magic Angle Spinning NMR Spectroscopy: A Versatile Technique for Structural and Dynamic Analysis of Solid-Phase Systems
From The DNP-NMR Blog:
Magic Angle Spinning NMR Spectroscopy: A Versatile Technique for Structural and Dynamic Analysis of Solid-Phase Systems
This review gives a comprehensive overview of the state-of-the-art of magic-angle spinning (MAS), solid-state NMR spectroscopy, including DNP-NMR spectroscopy.
1. Polenova, T., R. Gupta, and A. Goldbourt, Magic angle spinning NMR spectroscopy: a versatile technique for structural and dynamic analysis of solid-phase systems. Anal Chem, 2015. 87(11): p. 5458-69.
nmrlearner
News from NMR blogs
0
06-29-2015 07:21 PM
[NMR900 blog] New book: NMR Spectroscopy: A Versatile Tool for Environmental Research
New book: NMR Spectroscopy: A Versatile Tool for Environmental Research
Myrna J. Simpson (Editor)
Andre J. Simpson (Editor)
Hardcover: 448 pages
Publisher: Wiley; July 2014
Language: English
ISBN: 978-1118616475
http://ca.wiley.com/WileyCDA/WileyTitle/productCd-1118616472.html
http://www.amazon.com/dp/1118616472
nmrlearner
News from NMR blogs
0
06-30-2014 07:33 AM
[NMR analysis blog] Alignment of NMR spectra – Part IV: Advanced Alignment
Alignment of NMR spectra – Part IV: Advanced Alignment
Previous posts on this series:
Alignment of NMR spectra – Part I: The problem
Alignment of NMR spectra – Part II: Binning / Bucketing
Alignment of NMR spectra – Part III: Global Alignment
As I mentioned in my previous post, simple alignment based on shifting or referencing the whole spectrum is not enough in cases where there are different local chemical shift fluctuations.
Resorting back to the synthetic data set used in the previous posts, let me introduce a semi-automatic method designed specifically to align spectra having...
nmrlearner
News from NMR blogs
0
02-07-2011 07:53 PM
[NMR analysis blog] Alignment of NMR spectra – Part III: Global Alignment
Alignment of NMR spectra – Part III: Global Alignment
Previous posts on this series:
Alignment of NMR spectra – Part I: The problem
Alignment of NMR spectra – Part II: Binning / Bucketing
We have seen that binning helps in minimizing, for example, the effect of pH-induced fluctuations in chemical shift so that, in the field of NMR-based metabonomics studies, ensuring that signals for a given metabolite appear at the same location in all spectra. One evident disadvantage of binning is that it greatly reduces the spectral resolution (e.g. in a 500 MHz instrument, a typical 64 Kb...
Elongated Bacterial Pili as a Versatile Alignment Medium for NMR Spectroscopy
Linear Mode
