BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search
Home Forums Wiki NMR feeds Downloads Register Today's Posts



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
 
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Old 03-14-2013, 10:05 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,777
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Electron Transfer Mechanism of the Rieske Protein from Thermus thermophilus from Solution NMR investigations.

Electron Transfer Mechanism of the Rieske Protein from Thermus thermophilus from Solution NMR investigations.

Related Articles Electron Transfer Mechanism of the Rieske Protein from Thermus thermophilus from Solution NMR investigations.

Biochemistry. 2013 Mar 12;

Authors: Hsueh KL, Tonelli M, Cai K, Westler WM, Markley JL

Abstract
We report NMR data indicating that the Rieske protein from the cytochrome bc complex of Thermus thermophilus (TtRp) undergoes modest redox-state-dependent and ligand-dependent conformational changes. To test models concerning the mechanism by which TtRp transfers between different sites on the complex, we monitored (1)H, (15)N, and (13)C NMR signals as a function of redox state and molar ratio of added ligand. Our studies of full-length TtRp were carried out in the presence of dodecyl phosphocholine micelles to solvate the membrane anchor of the protein and the hydrophobic tail of the ligand (hydroubiquinone). NMR data indicated that hydroubiquinone binds to TtRp and stabilizes an altered protein conformation. We utilized a truncated form of the Rieske protein lacking the membrane anchor (trunc-TtRp) to investigate redox-state-dependent conformational changes. Local chemical shift perturbations suggested possible conformational changes at prolyl residues. Detailed investigations showed that all observable prolyl residues of oxidized trunc-TtRp have trans peptide bond configurations but that two of these peptide bonds (Cys151-Pro152 and Gly169-Pro170 located near the iron-sulfur cluster) become cis in the reduced protein. Changes in the chemical shifts of backbone signals provided evidence for redox-state- and ligand-dependent conformational changes localized near the iron-sulfur cluster. These structural changes may alter interactions between the Rieske protein and the cytochrome b and c sites and provide part of driving force for movement of the Rieske protein between these two sites.


PMID: 23480240 [PubMed - as supplied by publisher]



More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
NMR structure note: N-terminal domain of Thermus thermophilus CdnL
NMR structure note: N-terminal domain of Thermus thermophilus CdnL NMR structure note: N-terminal domain of Thermus thermophilus CdnL Content Type Journal Article Category NMR structure note Pages 1-9 DOI 10.1007/s10858-012-9648-z Authors
nmrlearner Journal club 0 07-13-2012 10:46 PM
Active site dynamics in NADH oxidase from Thermus thermophilus studied by NMR spin relaxation
Active site dynamics in NADH oxidase from Thermus thermophilus studied by NMR spin relaxation Abstract We have characterized the backbone dynamics of NADH oxidase from Thermus thermophilus (NOX) using a recently-developed suite of NMR experiments designed to isolate exchange broadening, together with 15N R 1, R 1Ï? , and {1H}-15N steady-state NOE relaxation measurements performed at 11.7 and 18.8 T. NOX is a 54 kDa homodimeric enzyme that belongs to a family of structurally homologous flavin reductases and nitroreductases with many potential biotechnology applications. Prior studies...
nmrlearner Journal club 0 09-30-2011 08:01 PM
[NMR paper] NMR investigations of allosteric processes in a two-domain Thermus thermophilus Hsp70
NMR investigations of allosteric processes in a two-domain Thermus thermophilus Hsp70 molecular chaperone. Related Articles NMR investigations of allosteric processes in a two-domain Thermus thermophilus Hsp70 molecular chaperone. J Mol Biol. 2005 May 27;349(1):163-83 Authors: Revington M, Zhang Y, Yip GN, Kurochkin AV, Zuiderweg ER Hsp70 chaperones are two-domain proteins that assist in intra-cellular protein (re) folding processes in all species. The protein folding activity of the substrate binding domain of the Hsp70s is regulated by...
nmrlearner Journal club 0 11-25-2010 08:21 PM
[NMR paper] NMR structure of the ribosomal protein L23 from Thermus thermophilus.
NMR structure of the ribosomal protein L23 from Thermus thermophilus. Related Articles NMR structure of the ribosomal protein L23 from Thermus thermophilus. J Biomol NMR. 2003 Jun;26(2):131-7 Authors: Ohman A, Rak A, Dontsova M, Garber MB, Härd T The ribosomal protein L23 is a component of the large ribosomal subunit in which it is located close to the peptide exit tunnel. In this position L23 plays a central role both for protein secretion and folding. We have determined the solution structure of L23 from Thermus thermophilus. Uncomplexed L23...
nmrlearner Journal club 0 11-24-2010 09:01 PM
[NMR paper] 31P-NMR spectroscopy of human and Paracoccus denitrificans electron transfer flavopro
31P-NMR spectroscopy of human and Paracoccus denitrificans electron transfer flavoproteins, and 13C- and 15N-NMR spectroscopy of human electron transfer flavoprotein in the oxidised and reduced states. Related Articles 31P-NMR spectroscopy of human and Paracoccus denitrificans electron transfer flavoproteins, and 13C- and 15N-NMR spectroscopy of human electron transfer flavoprotein in the oxidised and reduced states. Eur J Biochem. 1998 Jul 1;255(1):125-32 Authors: Griffin KJ, Degala GD, Eisenreich W, Müller F, Bacher A, Frerman FE Human and...
nmrlearner Journal club 0 11-17-2010 11:15 PM
[NMR paper] Preliminary NMR studies of Thermus thermophilus ribosomal protein S19 overproduced in
Preliminary NMR studies of Thermus thermophilus ribosomal protein S19 overproduced in Escherichia coli. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Preliminary NMR studies of Thermus thermophilus ribosomal protein S19 overproduced in Escherichia coli. FEBS Lett. 1997 Sep 29;415(2):155-9 Authors: Davydova NL, Rak AV, Gryaznova OI, Liljas A, Jonsson BH, Berglund H, Härd T, Garber MB The gene for the ribosomal protein S19 from Thermus thermophilus was cloned,...
nmrlearner Journal club 0 08-22-2010 05:08 PM
[NMR paper] 13C-NMR studies of transmembrane electron transfer to extracellular ferricyanide in h
13C-NMR studies of transmembrane electron transfer to extracellular ferricyanide in human erythrocytes. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 13C-NMR studies of transmembrane electron transfer to extracellular ferricyanide in human erythrocytes. Eur J Biochem. 1997 Jun 15;246(3):638-45 Authors: Himmelreich U, Kuchel PW Human erythrocytes are known to reduce ferricyanide (hexacyanoferrate) 3- to ferrocyanide 2- in an...
nmrlearner Journal club 0 08-22-2010 03:31 PM
[NMR paper] 13C-NMR studies of transmembrane electron transfer to extracellular ferricyanide in h
13C-NMR studies of transmembrane electron transfer to extracellular ferricyanide in human erythrocytes. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 13C-NMR studies of transmembrane electron transfer to extracellular ferricyanide in human erythrocytes. Eur J Biochem. 1997 Jun 15;246(3):638-45 Authors: Himmelreich U, Kuchel PW Human erythrocytes are known to reduce ferricyanide (hexacyanoferrate) 3- to ferrocyanide 2- in an...
nmrlearner Journal club 0 08-22-2010 03:03 PM



Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 02:52 PM.


Map