NMR paper An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors.

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[NMR paper] An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors.

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02-27-2016, 12:52 AM

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An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors.

An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors.

Related Articles An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors.

J Magn Reson. 2016 Jan 29;265:172-176

Authors: Hisao GS, Harland MA, Brown RA, Berthold DA, Wilson TE, Rienstra CM

Abstract
The study of mass-limited biological samples by magic angle spinning (MAS) solid-state NMR spectroscopy critically relies upon the high-yield transfer of material from a biological preparation into the MAS rotor. This issue is particularly important for maintaining biological activity and hydration of semi-solid samples such as membrane proteins in lipid bilayers, pharmaceutical formulations, microcrystalline proteins and protein fibrils. Here we present protocols and designs for rotor-packing devices specifically suited for packing hydrated samples into Pencil-style 1.6mm, 3.2mm standard, and 3.2mm limited speed MAS rotors. The devices are modular and therefore readily adaptable to other rotor and/or ultracentrifugation tube geometries.

PMID: 26905816 [PubMed - as supplied by publisher]

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