Related ArticlesEfficient Detection of Hydrogen Bonds in Dynamic Regions of RNA by Sensitivity-Optimized NMR Pulse Sequences.
Angew Chem Int Ed Engl. 2013 Aug 14;
Authors: Dallmann A, Simon B, Duszczyk MM, Kooshapur H, Pardi A, Bermel W, Sattler M
Abstract
Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs.
PMID: 23946052 [PubMed - as supplied by publisher]
[NMR paper] Ligand-induced strain in hydrogen bonds of the c-Src SH3 domain detected by NMR.
Ligand-induced strain in hydrogen bonds of the c-Src SH3 domain detected by NMR.
Related Articles Ligand-induced strain in hydrogen bonds of the c-Src SH3 domain detected by NMR.
J Mol Biol. 2000 Dec 8;304(4):497-505
Authors: Cordier F, Wang C, Grzesiek S, Nicholson LK
Changes in the molecular conformation of proteins can result from a variety of perturbations, and can play crucial roles in the regulation of biological activity. A new solution NMR method has been applied to monitor ligand-induced changes in hydrogen bond geometry in the...
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11-19-2010 08:29 PM
[NMR paper] Direct identification of NH...N hydrogen bonds in non-canonical base pairs of RNA by
Direct identification of NH...N hydrogen bonds in non-canonical base pairs of RNA by NMR spectroscopy.
Related Articles Direct identification of NH...N hydrogen bonds in non-canonical base pairs of RNA by NMR spectroscopy.
Nucleic Acids Res. 1999 Aug 1;27(15):3104-10
Authors: Wöhnert J, Dingley AJ, Stoldt M, Görlach M, Grzesiek S, Brown LR
It is shown that the recently developed quantitative J(NN)HNN-COSY experiment can be used for the direct identification of hydrogen bonds in non-canonical base pairs in RNA. Scalar(2h)J(NN)couplings across...
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11-18-2010 08:31 PM
[NMR paper] NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by
NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.
Related Articles NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14147-51
Authors: Pervushin K, Ono A, Fernández C, Szyperski T, Kainosho M, Wüthrich K
This paper describes the NMR observation of 15N---15N and 1H---15N scalar couplings across the hydrogen bonds in Watson-Crick base pairs...
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11-17-2010 11:15 PM
[NMR paper] Solution NMR characterization of hydrogen bonds in a protein by indirect measurement
Solution NMR characterization of hydrogen bonds in a protein by indirect measurement of deuterium quadrupole couplings.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Solution NMR characterization of hydrogen bonds in a protein by indirect measurement of deuterium quadrupole couplings.
J Magn Reson. 1997 Jul;127(1):54-64
Authors: Liwang AC, Bax A
Hydrogen bonds stabilize protein and nucleic acid structure, but little direct spectroscopic data have been available for...
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08-22-2010 03:31 PM
[NMR paper] Solution NMR characterization of hydrogen bonds in a protein by indirect measurement
Solution NMR characterization of hydrogen bonds in a protein by indirect measurement of deuterium quadrupole couplings.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Solution NMR characterization of hydrogen bonds in a protein by indirect measurement of deuterium quadrupole couplings.
J Magn Reson. 1997 Jul;127(1):54-64
Authors: Liwang AC, Bax A
Hydrogen bonds stabilize protein and nucleic acid structure, but little direct spectroscopic data have been available for...
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08-22-2010 03:03 PM
[NMR paper] H NMR probes for inter-segmental hydrogen bonds in myoglobins.
H NMR probes for inter-segmental hydrogen bonds in myoglobins.
Related Articles H NMR probes for inter-segmental hydrogen bonds in myoglobins.
J Biochem. 1996 Jul;120(1):126-32
Authors: Yamamoto Y
NMR signals arising from the HisB5 N delta H and HisEF5 N epsilon H protons in sperm whale skeletal and horse heart myoglobins have been located for the first time in the downfield shifted portion of the spectra. The shifts and hydrogen exchange rates indicate that these His imidazole ring NH protons are involved in the inter-segmental hydrogen bonds...
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08-22-2010 02:27 PM
[NMR paper] Transient hydrogen bonds identified on the surface of the NMR solution structure of H
Transient hydrogen bonds identified on the surface of the NMR solution structure of Hirudin.
Related Articles Transient hydrogen bonds identified on the surface of the NMR solution structure of Hirudin.
Biochemistry. 1994 Aug 9;33(31):9303-10
Authors: Szyperski T, Antuch W, Schick M, Betz A, Stone SR, Wüthrich K
Recombinant desulfatohirudin retains largely the thrombin-inhibitory activity of natural hirudin from Hirudo medicinalis and causes at most minimal immune response in humans. With regard to potential pharmaceutical applications it is...
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[Question from NMRWiki Q&A forum] How do you port pulse sequences to different field strength?
How do you port pulse sequences to different field strength?
Hi, when you set up a pulse sequence at a different magnet field strength - starting with an existing set of parameters - what do you look at?
Does length of shaped pulses always need to be changed?
What pulse lenghts need to be adjusted?
When it is necessary to re-build RF waveforms - for what kinds of pulses?
Efficient Detection of Hydrogen Bonds in Dynamic Regions of RNA by Sensitivity-Optimized NMR Pulse Sequences.
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