NMR paper Efficient chemical protein synthesis using Fmoc-masked N-terminal cysteine in Cys-peptide thioesters

		BioNMR > Educational resources > Journal club
[NMR paper] Efficient chemical protein synthesis using Fmoc-masked N-terminal cysteine in Cys-peptide thioesters

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-26-2020, 09:57 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Efficient chemical protein synthesis using Fmoc-masked N-terminal cysteine in Cys-peptide thioesters

Efficient chemical protein synthesis using Fmoc-masked N-terminal cysteine in Cys-peptide thioesters

Angewandte Chemie International Edition, Accepted Article.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Using Chemical Synthesis To Study and Apply Protein Glycosylation Using Chemical Synthesis To Study and Apply Protein Glycosylation http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b01055/20180114/images/medium/bi-2017-010556_0015.gif Biochemistry DOI: 10.1021/acs.biochem.7b01055 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/KmLgiLrr4Rc More...	nmrlearner	Journal club	0	01-17-2018 05:28 AM
[NMR paper] Palladium in Chemical Protein Synthesis and Modifications Palladium in Chemical Protein Synthesis and Modifications The field of site-specific modification of proteins has drawn significant attentions in recent years owing to its high importance in various research areas such as the development of novel therapeutics and understanding the biochemical and cellular behaviors of proteins. The presence of a large number of reactive functional groups in the protein of interest and in the cellular environment renders the particular modification at a specific site a highly challenging task. However, with the development of sophisticated...	nmrlearner	Journal club	0	04-07-2017 02:01 AM
Synthesis and Kinetic Analysis of Two ConformationallyRestricted Peptide Substrates of Escherichia coli Penicillin-Binding Protein 5 Synthesis and Kinetic Analysis of Two ConformationallyRestricted Peptide Substrates of Escherichia coli Penicillin-Binding Protein 5 http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00576/20160715/images/medium/bi-2016-00576v_0011.gif Biochemistry DOI: 10.1021/acs.biochem.6b00576 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/9GDEjEEdA6U More...	nmrlearner	Journal club	0	07-16-2016 04:54 AM
[NMR paper] An efficient fusion expression system for protein and peptide overexpression in Esche An efficient fusion expression system for protein and peptide overexpression in Escherichia coli and NMR sample preparation. Related Articles An efficient fusion expression system for protein and peptide overexpression in Escherichia coli and NMR sample preparation. Protein Pept Lett. 2003 Apr;10(2):175-81 Authors: Cheng Y, Liu D, Feng Y, Jing G An efficient fusion expression system with a small fusion partner, His6-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that...	nmrlearner	Journal club	0	11-24-2010 09:01 PM
[NMR paper] A simple efficient synthesis of [23,24]-(13)C(2)-labeled bile salts as NMR probes of A simple efficient synthesis of -(13)C(2)-labeled bile salts as NMR probes of protein-ligand interactions. Related Articles A simple efficient synthesis of -(13)C(2)-labeled bile salts as NMR probes of protein-ligand interactions. Bioorg Med Chem Lett. 2002 Feb 11;12(3):433-5 Authors: Tochtrop GP, DeKoster GT, Cistola DP, Covey DF The synthesis of -(13)C(2)-labeled bile salts is achieved through a steroidal side chain degradation and isotopic regeneration strategy. Three common bile acids were degraded to the corresponding C(22 )aldehyde by an...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR paper] Efficient solid-phase synthesis of Vpr from HIV-1 using low quantities of uniformly 1 Efficient solid-phase synthesis of Vpr from HIV-1 using low quantities of uniformly 13C-, 15N-labeled amino acids for NMR structural studies. Related Articles Efficient solid-phase synthesis of Vpr from HIV-1 using low quantities of uniformly 13C-, 15N-labeled amino acids for NMR structural studies. J Pept Res. 1999 Nov;54(5):427-35 Authors: Cornille F, Wecker K, Loffet A, Genet R, Roques B The 96-amino acid protein Vpr functions as a regulator of cellular processes involved in the human immunodeficiency virus, type 1 (HIV-1) life cycle,...	nmrlearner	Journal club	0	11-18-2010 08:31 PM
[NMR paper] Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. Related Articles Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. J Biomol NMR. 1997 Oct;10(3):245-53 Authors: Smith DE, Su JY, Jucker FM The power of heteronuclear NMR spectroscopy to study macromolecules and their complexes has been amply demonstrated over the last decade. The obstacle to routinely applying these techniques to the study of DNA has been the synthesis of 13C,15N-labeled DNA. Here we present a simple and efficient method to generate...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Cysteine pairing in the glycoprotein IIbIIIa antagonist kistrin using NMR, chemical a Cysteine pairing in the glycoprotein IIbIIIa antagonist kistrin using NMR, chemical analysis, and structure calculations. Related Articles Cysteine pairing in the glycoprotein IIbIIIa antagonist kistrin using NMR, chemical analysis, and structure calculations. Biochemistry. 1993 Jan 12;32(1):282-9 Authors: Adler M, Carter P, Lazarus RA, Wagner G The pairing of the cysteines in disulfide bonds was investigated for the 68-residue RGD-containing protein kistrin, a potent antagonist of the integrin GP IIbIIIa and an inhibitor of platelet...	nmrlearner	Journal club	0	08-21-2010 11:53 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off