Related ArticlesEfficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization.
Proc Natl Acad Sci U S A. 2017 May 01;:
Authors: Sergeyev IV, Itin B, Rogawski R, Day LA, McDermott AE
Abstract
An experimental strategy has been developed to increase the efficiency of dynamic nuclear polarization (DNP) in solid-state NMR studies. The method makes assignments simpler, faster, and more reliable via sequential correlations of both side-chain and C? resonances. The approach is particularly suited to complex biomolecules and systems with significant chemical-shift degeneracy. It was designed to overcome the spectral congestion and line broadening that occur due to sample freezing at the cryogenic temperatures required for DNP. Nonuniform sampling (NUS) is incorporated to achieve time-efficient collection of multidimensional data. Additionally, fast (25 kHz) magic-angle spinning (MAS) provides optimal sensitivity and resolution. Data collected in
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Dear Friends,
I am not able to figure out how to determine the side chain assignment of Last C-terminal SERINE residue of my protein. I can determine CA, CB, CO, N,H values from HNCA, CBCANH, HNCACO. Can someone tell which experiment will give me the information of HA, HB2 and HB3
Regards
Arun
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Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization.
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