[NMR paper] The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.
The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.
Related ArticlesThe effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.
Biochem Biophys Rep. 2016 Dec;8:75-80
Authors: Warner L, Gjersing E, Follett SE, Elliott KW, Dzyuba SV, Varga K
Abstract
Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect. However, on the molecular level, probing ionic liquid interactions with proteins, especially in solutions containing high concentration of ionic liquids, has been challenging. In the present work the (13)C, (15)N-enriched GB1 model protein was used to demonstrate applicability of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy to investigate ionic liquid - protein interactions. Effect of an ionic liquid (1-butyl-3-methylimidazolium bromide, [C4-mim]Br) on GB1was studied over a wide range of the ionic liquid concentrations (0.6 to 3.5 M, which corresponds to 10%-60% v/v). Interactions between GB1 and [C4-mim]Br were observed from changes in the chemical shifts of the protein backbone as well as the changes in (15)N ps-ns dynamics and rotational correlation times. Site-specific interactions between the protein and [C4-mim]Br were assigned using 3D methods under HR-MAS conditions. Thus, HR-MAS NMR is a viable tool that could aid in elucidation of the molecular mechanism of ionic liquid - protein interactions.
The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy
The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy
Publication date: Available online 11 August 2016
Source:Biochemistry and Biophysics Reports</br>
Author(s): Lisa Warner, Erica Gjersing, Shelby E. Follett, K. Wade Elliott, Sergei V. Dzyuba, Krisztina Varga</br>
Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect....
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[NMR paper] Effect of organochlorine pesticides exposure on the maize root metabolome assessed using high-resolution magic-angle spinning (1)H NMR spectroscopy.
Effect of organochlorine pesticides exposure on the maize root metabolome assessed using high-resolution magic-angle spinning (1)H NMR spectroscopy.
Effect of organochlorine pesticides exposure on the maize root metabolome assessed using high-resolution magic-angle spinning (1)H NMR spectroscopy.
Environ Pollut. 2016 Apr 28;214:539-548
Authors: Blondel C, Khelalfa F, Reynaud S, Fauvelle F, Raveton M
Abstract
(1)H-HRMAS NMR-based metabolomics was used to better understand the toxic effects on maize root tips of...
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05-02-2016 11:33 AM
[NMR paper] Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
NMR Biomed. 2015 Mar 12;
Authors: Tufts L, Shet Vishnudas K, Fu E, Kurhanewicz J, Ries M, Alliston T, Li X
Abstract
Osteoarthritis (OA) is a common multifactorial and heterogeneous degenerative joint disease, and biochemical changes in cartilage matrix occur during the early stages of OA before...
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03-13-2015 12:31 PM
[NMR paper] High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning.
High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning.
Related Articles High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning.
J Biomol NMR. 2013 Dec 13;
Authors: Ward ME, Wang S, Krishnamurthy S, Hutchins H, Fey M, Brown LS, Ladizhansky V
Abstract
Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the study of membrane proteins in membrane mimetic and native membrane...
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12-18-2013 04:00 PM
HistoneH3 and H4 N-Terminal Tails in NucleosomeArrays at Cellular Concentrations Probed by Magic Angle Spinning NMRSpectroscopy
HistoneH3 and H4 N-Terminal Tails in NucleosomeArrays at Cellular Concentrations Probed by Magic Angle Spinning NMRSpectroscopy
Min Gao, Philippe S. Nadaud, Morgan W. Bernier, Justin A. North, P. Chris Hammel, Michael G. Poirier and Christopher P. Jaroniec
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja407526s/aop/images/medium/ja-2013-07526s_0002.gif
Journal of the American Chemical Society
DOI: 10.1021/ja407526s
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/KRNVFRDIOnY
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[NMR paper] Histone H3 and H4 N-Terminal Tails in Nucleosome Arrays at Cellular Concentrations Probed by Magic Angle Spinning NMR Spectroscopy.
Histone H3 and H4 N-Terminal Tails in Nucleosome Arrays at Cellular Concentrations Probed by Magic Angle Spinning NMR Spectroscopy.
Histone H3 and H4 N-Terminal Tails in Nucleosome Arrays at Cellular Concentrations Probed by Magic Angle Spinning NMR Spectroscopy.
J Am Chem Soc. 2013 Oct 2;
Authors: Gao M, Nadaud PS, Bernier MW, North JA, Hammel PC, Poirier MG, Jaroniec CP
Abstract
Chromatin is a supramolecular assembly of DNA and histone proteins, organized into nucleosome repeat units. The dynamics of chromatin organization...
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10-04-2013 05:23 PM
[NMR paper] The assessment of the quality of the graft in an animal model for lung transplantation using the metabolomics 1H high-resolution magic angle spinning NMR spectroscopy.
The assessment of the quality of the graft in an animal model for lung transplantation using the metabolomics 1H high-resolution magic angle spinning NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles The assessment of the quality of the graft in an animal model for lung transplantation using the metabolomics 1H high-resolution magic angle spinning NMR spectroscopy.
Magn Reson Med. 2012 Oct;68(4):1026-38
Authors: Benahmed MA,...
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[NMR paper] Evidence of secondary structure by high-resolution magic angle spinning NMR spectrosc
Evidence of secondary structure by high-resolution magic angle spinning NMR spectroscopy of a bioactive peptide bound to different solid supports.
Related Articles Evidence of secondary structure by high-resolution magic angle spinning NMR spectroscopy of a bioactive peptide bound to different solid supports.
J Am Chem Soc. 2001 May 9;123(18):4130-8
Authors: Furrer J, Piotto M, Bourdonneau M, Limal D, Guichard G, Elbayed K, Raya J, Briand JP, Bianco A
The structure of the 19-amino acid peptide epitope, corresponding to the 141-159 sequence of...
The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.
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