[NMR paper] Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Biochemistry. 2017 Apr 05;:
Authors: Maria RM, Altei WF, Selistre-de-Araújo HS, Colnago LA
Abstract
Doxorubicin, cisplatin, and tamoxifen are part of many chemotherapeutic regimens. However, there are limited studies investigating the way metabolism in breast cancer is affected by chemotherapy. We studied, through 1H high-resolution magic angle spinning (HR-MAS) NMR spectroscopy, the metabolic profile of human breast cancer MCF-7 control (Con) cells as well as MCF-7 cells treated with tamoxifen (Tamo), cisplatin (Cis), and doxorubicin (Doxo). 1H HR-MAS NMR single pulse spectra evidenced signals from all mobile cell compounds, including fatty acids (membranes), water-soluble proteins, and metabolites. The spectra showed that phosphocholine (i.e., biomarker of breast cancer malignant transformation) signals were stronger in Con than in treated cells. Betaine (i.e., the major osmolyte in cells) was observed at similar concentrations in MCF-7 control and treated cells, but was absent in non-tumor MCF-10A cells. The NMR spectra acquired with Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence were used only in qualitative analyses because the signal areas were attenuated according to their transverse relaxation time (T2). The CPMG method was used to identify soluble metabolites such as organic acids, amino acids, choline and derivatives, taurine, and guanidinoacetate. 1H HR-MAS NMR spectroscopy efficiently demonstrated the effects of tamoxifen, cisplatin, and doxorubicin on the metabolic profile of MCF-7 cells. The fatty acid, phosphocholine, and choline variations observed by single pulse HR-MAS NMR have potential to characterize both responder and non-responder tumors in a molecular level.
PMID: 28379688 [PubMed - as supplied by publisher]
Understanding protein cross talk inside cells could lead to new breast cancer ... - Daily News - Galveston County
Understanding protein cross talk inside cells could lead to new breast cancer ... - Daily News - Galveston County
<img alt="" height="1" width="1" />
Understanding protein cross talk inside cells could lead to new breast cancer ...
Daily News - Galveston County
We are making progress in treatment and better diagnostics with advanced mammogram screening and the more refined NMR breast imaging. Together these are beginning to make a difference. And there are new promising techniques for specifically ...
and more »
Read here
nmrlearner
Online News
0
10-02-2014 06:36 AM
[NMR paper] Depletion of casein kinase I leads to a NAD(P)(+)/NAD(P)H balance-dependent metabolic adaptation as determined by NMR spectroscopy-metabolomic profile in Kluyveromyces lactis.
Depletion of casein kinase I leads to a NAD(P)(+)/NAD(P)H balance-dependent metabolic adaptation as determined by NMR spectroscopy-metabolomic profile in Kluyveromyces lactis.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Depletion of casein kinase I leads to a NAD(P)(+)/NAD(P)H balance-dependent metabolic adaptation as determined by NMR spectroscopy-metabolomic profile in Kluyveromyces lactis.
Biochim Biophys Acta. 2014 Jan;1840(1):556-64
Authors: Gorietti D, Zanni...
nmrlearner
Journal club
0
03-14-2014 07:34 PM
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
Abstract Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics technologies are capable of simultaneously measuring a wide range of metabolites in an untargeted manner, these methods have been relatively under utilized in the study of cancer cell responses to hypoxia. Thus, 1H NMR metabolomics was...
nmrlearner
Journal club
0
03-09-2011 04:19 AM
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
J Biomol NMR. 2011 Mar 4;
Authors: Weljie AM, Bondareva A, Zang P, Jirik FR
Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics...
nmrlearner
Journal club
0
03-05-2011 01:02 PM
Changes in the NMR metabolic profile of human microglial cells exposed to lipopolysaccharide or morphine.
Changes in the NMR metabolic profile of human microglial cells exposed to lipopolysaccharide or morphine.
Changes in the NMR metabolic profile of human microglial cells exposed to lipopolysaccharide or morphine.
J Neuroimmune Pharmacol. 2010 Dec;5(4):574-81
Authors: El Ghazi I, Sheng WS, Hu S, Reilly BG, Lokensgard JR, Rock RB, Peterson PK, Wilcox GL, Armitage IM
Microglial cells play a major role in host defense of the central nervous system. Once activated, several functional properties are up-regulated including migration, phagocytosis, and...
[NMR paper] Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR a
Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.
Related Articles Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.
Anticancer Res. 1996 May-Jun;16(3B):1375-80
Authors: Katz-Brull R, Degani H
The mechanism and kinetics of choline transport and phosphorylation in MCF7 human breast cancer cells was studied by 31P, 13C and 2H NMR, applying the zero trans method. Choline was transported by a...
Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Linear Mode
