NMR paper Effects of cholesterol on membrane molecular dynamics studied by fast field cycling NMR relaxometry.

		BioNMR > Educational resources > Journal club
[NMR paper] Effects of cholesterol on membrane molecular dynamics studied by fast field cycling NMR relaxometry.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-24-2013, 04:53 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Effects of cholesterol on membrane molecular dynamics studied by fast field cycling NMR relaxometry.

Effects of cholesterol on membrane molecular dynamics studied by fast field cycling NMR relaxometry.

Related Articles Effects of cholesterol on membrane molecular dynamics studied by fast field cycling NMR relaxometry.

Phys Chem Chem Phys. 2013 Aug 22;

Authors: Hsieh CJ, Chen YW, Hwang DW

Abstract
Biological membranes are complex structures composed of various lipids and proteins. Different membrane compositions affect viscoelastic and hydrodynamic properties of membranes, which are critical to their functions. Lipid bilayer vesicles inserted by cholesterol not only enhance membrane surface motional behavior but also strengthen vesicle stability. Cholesterol-rich vesicles are similar to cell membranes in structure and composition. Therefore, cholesterol-rich vesicles can represent a typical model for studying membrane dynamics and functions. In this study, nuclear magnetic relaxation dispersion was used to investigate the detailed molecular dynamics of membrane differences between vesicles and cholesterol vesicles in the temperature range of 278-298 K. Vesicles of two different sizes were prepared. The effect of cholesterol mainly affected the order fluctuation of membranes and the diffusional motion of lipid molecules. In addition, phase variations were also observed in liposomes that contained cholesterol from analyses of the distances between lipid molecules.

PMID: 23965762 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
From the Micelle to the Membrane: Molecular Dynamics Simulations of Solution NMR Structures of Membrane Proteins From the Micelle to the Membrane: Molecular Dynamics Simulations of Solution NMR Structures of Membrane Proteins 29 January 2013 Publication year: 2013 Source:Biophysical Journal, Volume 104, Issue 2, Supplement 1</br> </br> </br> </br></br>	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Field-cycling NMR relaxometry of viscous liquids and polymers Field-cycling NMR relaxometry of viscous liquids and polymers May 2012 Publication year: 2012 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 63</br> </br> Graphical abstract	nmrlearner	Journal club	0	12-15-2012 09:51 AM
Field-cycling NMR relaxometry of viscous liquids and polymers Field-cycling NMR relaxometry of viscous liquids and polymers May 2012 Publication year: 2012 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 63</br> </br> Graphical abstract	nmrlearner	Journal club	0	12-01-2012 06:10 PM
Broadband finite-pulse radio-frequency-driven recoupling (fp-RFDR) with (XY8)41 super-cycling for homo-nuclear correlations in very high magnetic fields at fast and ultra-fast MAS frequencies Broadband finite-pulse radio-frequency-driven recoupling (fp-RFDR) with (XY8)41 super-cycling for homo-nuclear correlations in very high magnetic fields at fast and ultra-fast MAS frequencies Publication year: 2012 Source:Journal of Magnetic Resonance</br> Ming Shen, Bingwen Hu, Oliver Lafon, Julien Trébosc, Qun Chen, Jean-Paul Amoureux</br> We demonstrate that inter-residue 13C-13C proximities (of about 380 pm) in uniformly 13C-labeled proteins can be probed by applying robust first-order recoupling during several milliseconds in single-quantum single-quantum...	nmrlearner	Journal club	0	07-28-2012 01:35 AM
Field-cycling NMR relaxometry of viscous liquids and polymers Field-cycling NMR relaxometry of viscous liquids and polymers Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> D. Kruk, A. Herrmann, E.A. Rössler</br> Graphical Abstract http://ars.sciencedirect.com/content/image/1-s2.0-S0079656511000586-fx1.jpg Graphical abstract Highlights	nmrlearner	Journal club	0	03-09-2012 09:16 AM
Field-cycling NMR relaxometry of viscous liquids and polymers Field-cycling NMR relaxometry of viscous liquids and polymers Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 27 August 2011</br> D., Kruk , A., Herrmann , E.A., Rössler</br> Graphical abstract Graphical abstract:Highlights:? NMR relaxometry compared with DQ NMR, dielectric spectroscopy and light scattering ? Applying susceptibility representation and frequency-temperature superposition ? Liquids: Intra- & intermolecular relaxation give rotational & translational correlation times ? Polymers:...	nmrlearner	Journal club	0	08-29-2011 06:41 AM
[NMR paper] High-resolution 31p field cycling NMR as a probe of phospholipid dynamics. High-resolution 31p field cycling NMR as a probe of phospholipid dynamics. Related Articles High-resolution 31p field cycling NMR as a probe of phospholipid dynamics. J Am Chem Soc. 2004 Oct 27;126(42):13765-77 Authors: Roberts MF, Redfield AG We have used high-resolution field-cycling 31P NMR spectroscopy to measure spin-lattice relaxation rates (R1 = 1/T1) of multicomponent phospholipid vesicle and micelle samples over a large field range, from 0.1 to 11.7 T. The shape of the curve for R1 as a function of field and a model-free analysis were...	nmrlearner	Journal club	0	11-24-2010 10:01 PM
[NMR tweet] Radiology publications: Desktop fast-field cycling nuclear magnetic resonance relaxom Radiology publications: Desktop fast-field cycling nuclear magnetic resonance relaxometer. ... http://bit.ly/dmjaCl Source: Twitter	nmrlearner	Twitter NMR	0	08-14-2010 02:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off