The evaluation of joint disease using synovial fluid is an emerging field of metabolic profiling. The analysis is challenged by multiple macromolecules which can obscure the small molecule chemistry. The use of protein precipitation and extraction has been evaluated previously, but not in synovial fluid. We systematically review the published NMR spectroscopy methods of synovial fluid analysis and investigated the efficacy of three different protein precipitation techniques: methanol,...
[NMR paper] 1H-NMR metabolomic profile of healthy and osteoarthritic canine synovial fluid before and after UC-II supplementation
1H-NMR metabolomic profile of healthy and osteoarthritic canine synovial fluid before and after UC-II supplementation
The aim of the study was to compare the metabolomic synovial fluid (SF) profile of dogs affected by spontaneous osteoarthritis (OA) and supplemented with undenatured type II collagen (UC-II), with that of healthy control dogs. Client-owned dogs were enrolled in the study and randomized in two different groups, based on the presence/absence of OA (OA group and OA-free group). All dogs were clinically evaluated and underwent SF sampling for ¹H-Nuclear Magnetic Resonance...
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11-21-2022 10:34 PM
[NMR paper] Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy
Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy
Liquid-liquid phase separation (LLPS) of protein solutions is increasingly recognised as an important phenomenon in cell biology and biotechnology. However, opalescence and concentration fluctuations render LLPS difficult to study, particularly when characterising the kinetics of the phase transition and layer separation. Here, we demonstrate the use of a probe molecule trifluoroethanol (TFE) to characterise the kinetics of protein LLPS by NMR spectroscopy. The chemical shift and...
[NMR paper] Optimisation of Synovial Fluid Collection and Processing for NMR Metabolomics and LC-MS/MS Proteomics.
Optimisation of Synovial Fluid Collection and Processing for NMR Metabolomics and LC-MS/MS Proteomics.
Related Articles Optimisation of Synovial Fluid Collection and Processing for NMR Metabolomics and LC-MS/MS Proteomics.
J Proteome Res. 2020 Mar 31;:
Authors: Anderson JR, Phelan MM, Rubio-Martinez LM, Fitzgerald MM, Jones SW, Clegg PD, Peffers MJ
Abstract
Synovial fluid (SF) is of great interest for the investigation of orthopaedic pathologies as it is in close proximity to various tissues which are primarily altered during...
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04-02-2020 06:25 PM
[ASAP] Pressure-Sensitive and Osmolyte-Modulated Liquid–Liquid Phase Separation of Eye-Lens ?-Crystallins
Pressure-Sensitive and Osmolyte-Modulated Liquid–Liquid Phase Separation of Eye-Lens ?-Crystallins
Süleyman Cinar, Hasan Cinar, Hue Sun Chan, Roland Winter
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b13636/20190423/images/medium/ja-2018-13636x_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.8b13636
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/0k8PRwEgdm4
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04-24-2019 09:00 AM
[NMR paper] Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Mol Pharm. 2017 Jun 14;:
Authors: Kheddo P, Bramham JE, Dearman RJ, Uddin S, van der Walle CF, Golovanov AP
Abstract
Liquid-liquid phase separation (LLPS) of monoclonal antibody (mAb) formulations involves spontaneous separation into dense...
The effect of liquid-liquid extraction on metabolite detection and analysis using NMR spectroscopy in human synovial fluid
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