NMR paper Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of p

		BioNMR > Educational resources > Journal club
[NMR paper] Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of p

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 04:03 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of p

Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of protein-water interactions.

Related Articles Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of protein-water interactions.

J Magn Reson. 1999 Feb;136(2):214-8

Authors: Melacini G, Kaptein R, Boelens R

An experimental approach for the editing of exchange-relayed NOEs in water-selective NOE experiments is presented. The proposed pulse sequence is based on the application during the NOE mixing time of continuous wave irradiation, which saturates resonances of relaying labile protons in slow chemical exchange with water. The technique can efficiently reduce the contributions of exchange-relayed NOE peaks that often crowd the water-selective NOE spectra and hide direct intermolecular NOEs between water and protein protons. The present approach opens new opportunities for the characterization of hydration by NMR, even in the proximity of polar labile groups.

PMID: 9986764 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR thesis] I. Quantum-mechanical chemical exchange. II. NMR of semiconductors I. Quantum-mechanical chemical exchange. II. NMR of semiconductors Kurur, Narayanan Damodaran (1992) I. Quantum-mechanical chemical exchange. II. NMR of semiconductors. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:09022011-090934651 More...	nmrlearner	NMR theses	0	09-02-2011 07:31 PM
Challenges in numerical simulations of solid-state NMR experiments: Spin exchange pulse sequences. Challenges in numerical simulations of solid-state NMR experiments: Spin exchange pulse sequences. Challenges in numerical simulations of solid-state NMR experiments: Spin exchange pulse sequences. Solid State Nucl Magn Reson. 2011 Feb 1; Authors: Vosegaard T While simulations are essential for interpretation of solid-state NMR experiments, large spin systems involved in e.g. spin-diffusion experiments and/or dynamic effects like chemical exchange pose great challenges for the numerical simulations, where we typically want to include effects of...	nmrlearner	Journal club	0	02-19-2011 06:02 PM
TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins Abstract A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of 15N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232â??13237, 2007), by preserving the TROSY effect during the mixing...	nmrlearner	Journal club	0	01-09-2011 12:46 PM
[NMR paper] High-sensitivity observation of dipolar exchange and NOEs between exchangeable proton High-sensitivity observation of dipolar exchange and NOEs between exchangeable protons in proteins by 3D solid-state NMR spectroscopy. Related Articles High-sensitivity observation of dipolar exchange and NOEs between exchangeable protons in proteins by 3D solid-state NMR spectroscopy. J Am Chem Soc. 2003 Nov 26;125(47):14222-3 Authors: Paulson EK, Morcombe CR, Gaponenko V, Dancheck B, Byrd RA, Zilm KW A highly sensitive new 1H-detected 3D solid-state NMR method is described for characterizing 1H-1H spin exchange in nanocrystalline samples of...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Observation of slow dynamic exchange processes in Ras protein crystals by 31P solid s Observation of slow dynamic exchange processes in Ras protein crystals by 31P solid state NMR spectroscopy. Related Articles Observation of slow dynamic exchange processes in Ras protein crystals by 31P solid state NMR spectroscopy. J Mol Biol. 2002 Nov 8;323(5):899-907 Authors: Stumber M, Geyer M, Graf R, Kalbitzer HR, Scheffzek K, Haeberlen U The folding, structure and biological function of many proteins are inherently dynamic properties of the protein molecule. Often, the respective molecular processes are preserved upon protein...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] New (15)N NMR exchange experiments for the unambiguous assignment of (1)H(N)/(15)N re New (15)N NMR exchange experiments for the unambiguous assignment of (1)H(N)/(15)N resonances of proteins in complexes in slow chemical exchange with free form. Related Articles New (15)N NMR exchange experiments for the unambiguous assignment of (1)H(N)/(15)N resonances of proteins in complexes in slow chemical exchange with free form. J Magn Reson. 2000 Feb;142(2):276-9 Authors: Vialle-Printems C, van Heijenoort C, Guittet E The potentialities of a 2D proton-detected heteronuclear exchange experiment to assign the nitrogen and amide proton...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
[Question from NMRWiki Q&A forum] Relaxation editing vr paramagnetic relaxation enhancement experiments - 13C CP-MAS NM Relaxation editing vr paramagnetic relaxation enhancement experiments - 13C CP-MAS NMR I am a beginner in NMR spectroscopy and I would like to learn more about relaxation editing experiments vs PRE. A colleague of mine is doing the 13C CP-MAS NMR experim. and he using cellulose II powder, regenerated cellulose and milled reg cellulose. We are interested in C4 resonance of cellulose II, good resolved resonance, to better understand the supramolecular structure of cellulose II. As experiments: long relaxation experiments - PRE with aqueous CuSO4 solution of certain concentration, does the...	nmrlearner	News from other NMR forums	0	10-15-2010 05:16 PM
Suite of Six NMR Relaxation Dispersion Experiments to Study Multiple-Site Exchange in Proteins http://pubs.acs.org/isubscribe/journals/jacsat/127/i44/figures/ja054550en00001.gif Multiple-Site Exchange in Proteins Studied with a Suite of Six NMR Relaxation Dispersion Experiments: An Application to the Folding of a Fyn SH3 Domain Mutant Dmitry M. Korzhnev, Philipp Neudecker, Anthony Mittermaier, Vladislav Yu. Orekhov, and Lewis E. Kay* Contribution from the Departments of Medical Genetics, Biochemistry, and Chemistry, The University of Toronto, Toronto, Ontario M5S 1A8, Canada, and Swedish NMR Center at Göteborg University, Box 465, 405 30 Göteborg, Sweden J. Am. Chem....	nmrlearner	Journal club	0	01-12-2006 08:33 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off