NMR paper E6AP AZUL interaction with UBQLN1/2 in cells, condensates, and an AlphaFold-NMR integrated structure

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[NMR paper] E6AP AZUL interaction with UBQLN1/2 in cells, condensates, and an AlphaFold-NMR integrated structure

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NMR processing:

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NOEs:

Structure from NMR restraints:

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GeNMR

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Amber

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Promega- Proline

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CSI (via RCI server)

TALOS

MICS caps, β-turns

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HADDOCK

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B-factor

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CH3shift- Methyl

ArShift- Aromatic

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Proshift

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Shifty

Camcoil

Poulsen_rc_CS

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02-27-2023, 04:40 PM

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E6AP AZUL interaction with UBQLN1/2 in cells, condensates, and an AlphaFold-NMR integrated structure

E6AP AZUL interaction with UBQLN1/2 in cells, condensates, and an AlphaFold-NMR integrated structure

The E3 ligase E6AP/UBE3A has a dedicated binding site in the 26S proteasome provided by the RAZUL domain of substrate receptor hRpn10/S5a/PSMD4. Guided by RAZUL sequence similarity, we test and demonstrate here that the E6AP AZUL binds transiently to the UBA of proteasomal shuttle factor UBQLN1/2. Despite a weak binding affinity, E6AP AZUL is recruited to UBQLN2 biomolecular condensates in vitro and E6AP interacts with UBQLN1/2 in cellulo. Steady-state and transfer nuclear Overhauser effect...

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