â??CON-CONâ?? assignment strategy for highly flexible intrinsically disordered proteins
â??CON-CONâ?? assignment strategy for highly flexible intrinsically disordered proteins
Abstract
Intrinsically disordered proteins (IDPs) are a class of highly flexible proteins whose characterization by NMR spectroscopy is complicated by severe spectral overlaps. The development of experiments designed to facilitate the sequence-specific assignment procedure is thus very important to improve the tools for the characterization of IDPs and thus to be able to focus on IDPs of increasing size and complexity. Here, we present and describe the...
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10-21-2014 11:31 PM
A General Method for Constructing Atomic-ResolutionRNA Ensembles using NMR Residual Dipolar Couplings: The Basis forInterhelical Motions Revealed
A General Method for Constructing Atomic-ResolutionRNA Ensembles using NMR Residual Dipolar Couplings: The Basis forInterhelical Motions Revealed
Loi?c Salmon, Gavin Bascom, Ioan Andricioaei and Hashim M. Al-Hashimi
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja400920w/aop/images/medium/ja-2013-00920w_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja400920w
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/hvS4n8AO9ys
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03-29-2013 07:07 AM
[NMR paper] A General Method for Constructing Atomic-Resolution RNA Ensembles using NMR Residual Dipolar Couplings: The Basis for Inter-helical Motions Revealed.
A General Method for Constructing Atomic-Resolution RNA Ensembles using NMR Residual Dipolar Couplings: The Basis for Inter-helical Motions Revealed.
Related Articles A General Method for Constructing Atomic-Resolution RNA Ensembles using NMR Residual Dipolar Couplings: The Basis for Inter-helical Motions Revealed.
J Am Chem Soc. 2013 Mar 8;
Authors: Salmon L, Bascom GD, Andricioaei I, Al-Hashimi HM
Abstract
The ability to modulate alignment and measure multiple independent sets of NMR residual dipolar couplings (RDCs) has made it possible...
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03-12-2013 07:09 PM
Very large residual dipolar couplings from deuterated ubiquitin
Very large residual dipolar couplings from deuterated ubiquitin
Abstract Main-chain 1HNâ??15N residual dipolar couplings (RDCs) ranging from approximately â??200 to 200 Hz have been measured for ubiquitin under strong alignment conditions in Pf1 phage. This represents a ten-fold increase in the degree of alignment over the typical weakly aligned samples. The measurements are made possible by extensive proton-dilution of the sample, achieved by deuteration of the protein with partial back-substitution of labile protons from 25 % H2O / 75 % D2O buffer. The spectral quality is further...
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07-30-2012 07:42 AM
Residual dipolar couplings: are multiple independent alignments always possible?
Residual dipolar couplings: are multiple independent alignments always possible?
Abstract RDCs for the 14 kDa protein hen egg-white lysozyme (HEWL) have been measured in eight different alignment media. The elongated shape and strongly positively charged surface of HEWL appear to limit the protein to four main alignment orientations. Furthermore, low levels of alignment and the proteinâ??s interaction with some alignment media increases the experimental error. Together with heterogeneity across the alignment media arising from constraints on temperature, pH and ionic strength for some...
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12-26-2010 04:43 AM
[NMR paper] Refined NMR structure of alpha-sarcin by 15N-1H residual dipolar couplings.
Refined NMR structure of alpha-sarcin by 15N-1H residual dipolar couplings.
Related Articles Refined NMR structure of alpha-sarcin by 15N-1H residual dipolar couplings.
Eur Biophys J. 2005 Nov;34(8):1057-65
Authors: García-Mayoral MF, Pantoja-Uceda D, Santoro J, Martínez del Pozo A, Gavilanes JG, Rico M, Bruix M
(15)N-(1)H residual dipolar couplings (RDC) have been used as additional restraints to refine the solution structure of the ribotoxin alpha-sarcin. The RDC values were obtained by partial alignment of alpha-sarcin in the binary mixture...
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11-25-2010 08:21 PM
[NMR paper] Residual dipolar couplings in NMR structure analysis.
Residual dipolar couplings in NMR structure analysis.
Related Articles Residual dipolar couplings in NMR structure analysis.
Annu Rev Biophys Biomol Struct. 2004;33:387-413
Authors: Lipsitz RS, Tjandra N
Residual dipolar couplings (RDCs) have recently emerged as a new tool in nuclear magnetic resonance (NMR) with which to study macromolecular structure and function in a solution environment. RDCs are complementary to the more conventional use of NOEs to provide structural information. While NOEs are local-distance restraints, RDCs provide...
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11-24-2010 09:25 PM
[NMR paper] Residual dipolar couplings: synergy between NMR and structural genomics.
Residual dipolar couplings: synergy between NMR and structural genomics.
Related Articles Residual dipolar couplings: synergy between NMR and structural genomics.
J Biomol NMR. 2002 Jan;22(1):1-8
Authors: Al-Hashimi HM, Patel DJ
Structural genomics is on a quest for the structure and function of a significant fraction of gene products. Current efforts are focusing on structure determination of single-domain proteins, which can readily be targeted by X-ray crystallography, NMR spectroscopy and computational homology modeling. However,...