Related ArticlesDynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses.
Nat Commun. 2017 Feb 22;8:14523
Authors: Toyama Y, Kano H, Mase Y, Yokogawa M, Osawa M, Shimada I
Abstract
Heterotrimeric guanine-nucleotide-binding proteins (G proteins) serve as molecular switches in signalling pathways, by coupling the activation of cell surface receptors to intracellular responses. Mutations in the G protein ?-subunit (G?) that accelerate guanosine diphosphate (GDP) dissociation cause hyperactivation of the downstream effector proteins, leading to oncogenesis. However, the structural mechanism of the accelerated GDP dissociation has remained unclear. Here, we use magnetic field-dependent nuclear magnetic resonance relaxation analyses to investigate the structural and dynamic properties of GDP bound G? on a microsecond timescale. We show that G? rapidly exchanges between a ground-state conformation, which tightly binds to GDP and an excited conformation with reduced GDP affinity. The oncogenic D150N mutation accelerates GDP dissociation by shifting the equilibrium towards the excited conformation.
Identificationof Dynamic Modes in an IntrinsicallyDisordered Protein Using Temperature-Dependent NMR Relaxation
Identificationof Dynamic Modes in an IntrinsicallyDisordered Protein Using Temperature-Dependent NMR Relaxation
Anton Abyzov, Nicola Salvi, Robert Schneider, Damien Maurin, Rob W.H. Ruigrok, Malene Ringkjøbing Jensen and Martin Blackledge
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.6b02424/20160506/images/medium/ja-2016-02424a_0010.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.6b02424
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http://feeds.feedburner.com/~r/acs/jacsat/~4/MZI5nf_B2ds
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[NMR paper] Identification of Dynamic Modes in an Intrinsically Disordered Protein using Temperature-dependent NMR Relaxation.
Identification of Dynamic Modes in an Intrinsically Disordered Protein using Temperature-dependent NMR Relaxation.
Related Articles Identification of Dynamic Modes in an Intrinsically Disordered Protein using Temperature-dependent NMR Relaxation.
J Am Chem Soc. 2016 Apr 26;
Authors: Abyzov A, Salvi N, Schneider R, Maurin D, Ruigrok RW, Jensen MR, Blackledge M
Abstract
[NMR paper] Detecting a New Source for Photochemically Induced Dynamic Nuclear Polarization in the LOV2 Domain of Phototropin by Magnetic-Field Dependent (13)C-NMR Spectroscopy.
Detecting a New Source for Photochemically Induced Dynamic Nuclear Polarization in the LOV2 Domain of Phototropin by Magnetic-Field Dependent (13)C-NMR Spectroscopy.
Related Articles Detecting a New Source for Photochemically Induced Dynamic Nuclear Polarization in the LOV2 Domain of Phototropin by Magnetic-Field Dependent (13)C-NMR Spectroscopy.
J Phys Chem B. 2014 Sep 10;
Authors: Kothe G, Lukaschek M, Link G, Kacprzak S, Illarionov B, Fischer M, Eisenreich W, Bacher A, Weber S
Abstract
Phototropin is a flavin mononucleotide...
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MD andNMR Analyses of Choline and TMA Binding toDuplex DNA: On the Origins of Aberrant Sequence-Dependent Stabilityby Alkyl Cations in Aqueous and Water-Free Solvents
MD andNMR Analyses of Choline and TMA Binding toDuplex DNA: On the Origins of Aberrant Sequence-Dependent Stabilityby Alkyl Cations in Aqueous and Water-Free Solvents
Guillem Portella, Markus W. Germann, Nicholas V. Hud and Modesto Orozco
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja410698u/aop/images/medium/ja-2013-10698u_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/ja410698u
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http://feeds.feedburner.com/~r/acs/jacsat/~4/Hr1kPtKr_y8
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[NMR paper] Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-induced Rat Pleurisy.
Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-induced Rat Pleurisy.
Related Articles Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-induced Rat Pleurisy.
J Proteome Res. 2013 Oct 17;
Authors: Li H, An Y, Zhang L, Lei H, Zhang L, Wang Y, Tang H
Abstract
Inflammation is closely associated with pathogenesis of various metabolic disorders, cardiovascular diseases and cancers. To understand the systems responses to localized inflammation, we...
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10-18-2013 06:23 PM
Low-field one-dimensional and direction-dependent relaxation imaging of bovine articular cartilage
Low-field one-dimensional and direction-dependent relaxation imaging of bovine articular cartilage
Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 10 September 2011</br>
Erik*Rössler, Carlos*Mattea, Ayret*Mollova, Siegfried*Stapf</br>
The structure of articular cartilage is separated into three layers of differently oriented collagen fibers, which is accompanied by a gradient of increasing glycosaminoglycan (GAG) and decreasing water concentration from the top layer towards the bone interface. The combined effect of these structural variations results in...
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[NMR paper] Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and
Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and line-shape analysis.
Related Articles Backbone motions in a crystalline protein from field-dependent 2H-NMR relaxation and line-shape analysis.
Biopolymers. 2000 Jan;53(1):9-18
Authors: Mack JW, Usha MG, Long J, Griffin RG, Wittebort RJ
We have used 2H-nmr to study backbone dynamics of the 2H-labeled, slowly exchanging amide sites of fully hydrated, crystalline hen egg white lysozyme. Order parameters are determined from the residual quadrupole coupling and...
Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses.
Linear Mode
