The M121H azurin mutant in solution presents various species in equilibrium that can be detected and studied by 1H NMR of the Cu(II) and Co(II) paramagnetic metalloderivatives. In both cases up to three species are observed in slow exchange, the proportions of which are different for the two metalloderivatives. Above pH 5 the major species displays a tetrahedral coordination in which the His121 can be observed as a coordinated residue. Its metal site corresponds to a new type of site that is defined as a type 1.5 site. The second and third species resemble the wild type (type 1) azurin and, above pH 4.5, they are present only at a low concentration. At low pH a protonation process increases the proportion of both type 1 species at the expense of the type 1.5 species. This process, characterized by a pKa = 4.3, is assigned to the protonation of His121. At high pH the NMR spectrum of the Co(II)-M121H azurin experiences an additional transition, which is not observed in the case of the Cu(II) protein. The dynamic properties of the M121H metal site appear to be related to changes in the coordination geometry and the strength of the axial interaction between the Ndelta1 (His121) and the metal.
[NMR paper] Investigating the dynamic properties of the transmembrane segment of phospholamban in
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(2)H and (15)N solid-state NMR spectroscopic techniques were used to investigate...
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[NMR paper] Gated electron transfers and electron pathways in azurin: a NMR dynamic study at mult
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[NMR paper] Metal-ligand interplay in blue copper proteins studied by 1H NMR spectroscopy: Cu(II)
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J Am Chem Soc. 2002 Nov 20;124(46):13698-708
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[NMR paper] Structure of the metal-water complex in Ras x GDP studied by high-field EPR spectrosc
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Biochemistry. 2001 Feb 20;40(7):1884-9
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The small GTPase Ras plays a key role as a molecular switch in the intercellular signal transduction. On Mg(2+) --> Mn(2+) substituted samples, the first ligand sphere of...
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[NMR paper] Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P N
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J Biochem. 1994 Feb;115(2):270-8
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The dynamic properties of nucleic acids in five different types of intact supramolecular systems, namely, chicken erythrocyte chromatin, the wild type and a deletion mutant of the lambda phage, lipid-containing phage PM2, and...
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[NMR paper] Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P N
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J Biochem. 1994 Feb;115(2):270-8
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Two-dimensional proton-detected heteronuclear nuclear magnetic resonance spectroscopy has been used to measure 13C and 15N spin-relaxation rate constants for several proteins. Generalized order parameters and effective internal correlation times have been...
The dynamic properties of the M121H azurin metal site as studied by NMR of the parama
Linear Mode
